Abstract:
:During 2003, surveys of sugarcane yellow leaf disease and papaya bunchy top-like disease were carried out on plantations in Havana province, Cuba, to determine the roles of weeds and Auchenorrhyncha insects in the epidemiology of these diseases. More than 250 plant and insect samples were collected and indexed by using a nested PCR for phytoplasma 16S rDNA with the generic primer pairs P1/P7 and R16F2n/R16R2. The PCR products were further characterized by restriction fragment length polymorphism using HaeIII, AluI, Sau3AI, Tru9I, HhaI, HpaII and TaqI endonucleases, giving patterns that distinguished them from those of the other reference phytoplasmas analysed. Phylogenetic analysis of 16S rRNA gene sequences identified the phytoplasmas present in sugarcane (Saccharum officinarum L.), Cynodon dactylon L., Conyza canadensis L. Cronq., Sorghum halepense L. Pers., Macroptilium lathyroides L. Urb., Saccharosydne saccharivora (Westwood) and Cedusa spp., and those in papaya (Carica papaya L.) and Empoasca papayae, as two novel provisional phytoplasma species. We propose that these phytoplasmas should be given Candidatus status, as 'Candidatus Phytoplasma graminis' and 'Candidatus Phytoplasma caricae', respectively.
journal_name
Int J Syst Evol Microbiolauthors
Arocha Y,López M,Piñol B,Fernández M,Picornell B,Almeida R,Palenzuela I,Wilson MR,Jones Pdoi
10.1099/ijs.0.63797-0keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
2451-2463issue
Pt 6eissn
1466-5026issn
1466-5034journal_volume
55pub_type
杂志文章abstract::The placement of 'Pseudomonas butanovora' in the genus Thauera was proposed previously, based on 16S rRNA gene sequence analysis, upon further studies of taxonomical characteristics. In this study, physiological characteristics and DNA-DNA reassociation data are presented and the transfer of 'P. butanovora' to the gen...
journal_title:International journal of systematic and evolutionary microbiology
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doi:10.1099/ijs.0.000638-0
更新日期:2009-07-01 00:00:00
abstract::Two novel facultatively anaerobic bacterial strains, BL-34(T) and BL-35, isolated from groundwater contaminated by a mixture of chlorosolvents were characterized using a polyphasic approach. The two strains exhibited essentially identical taxonomic features except for a vitamin B(12) requirement by strain BL-35 for op...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.64317-0
更新日期:2006-08-01 00:00:00
abstract::A novel Gram-staining-negative, strictly aerobic bacterium, designated BS14(T), was isolated from a marine tidal flat of the South Sea in Korea. Colonies were opaque, white, smooth and circular on marine agar. Cells were moderately halophilic, non-motile rods showing catalase- and oxidase-positive reactions. Growth of...
journal_title:International journal of systematic and evolutionary microbiology
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doi:10.1099/ijs.0.048389-0
更新日期:2013-08-01 00:00:00
abstract::Two strains of lactic acid bacteria, Nu27(T) and Nu29, were isolated from Nukadoko, rice bran paste for Japanese pickles. The isolates were Gram-stain-positive, rod-shaped, catalase-negative, non-motile and facultatively anaerobic lactic acid bacteria. The isolates showed identical 16S rRNA gene sequences. The closest...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
abstract::During a study of yeast diversity in marine waters of the Arabian Gulf surrounding Qatar, 30 strains were isolated that represent a novel species of Kondoa. The isolates were obtained from five locations along the Qatari coast. Species identifications were based on sequence analysis of the internal transcribed spacer ...
journal_title:International journal of systematic and evolutionary microbiology
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doi:10.1099/ijsem.0.003182
更新日期:2019-02-01 00:00:00
abstract::Biochemical and molecular genetic studies were performed on an unknown Gram-positive, catalase-negative, coccus-shaped organism isolated from clinical samples from pigs. On the basis of the results of cellular morphological and biochemical tests, the organism was identified as a streptococcal species. 16S rRNA gene se...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65636-0
更新日期:2009-03-01 00:00:00
abstract::A mesophilic hydrogenotrophic methanogen, strain MHT-1T, was isolated from the leachate of a sea-based site for solid waste disposal (the port of Osaka, Japan). Strain MHT-1T was found to be an irregular coccus and was able to use H2/CO2 and formate as energy sources. Acetate was required for growth. The optimum tempe...
journal_title:International journal of systematic and evolutionary microbiology
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doi:10.1099/00207713-50-5-1723
更新日期:2000-09-01 00:00:00
abstract::A bacterial strain, designated 2DFW10M-5T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea. Cells of the strain were Gram-stain-positive, non-motile and rod-shaped. The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and...
journal_title:International journal of systematic and evolutionary microbiology
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doi:10.1099/ijsem.0.003767
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abstract::A high-G+C-content, Gram-positive bacterium, strain D10(T), was isolated from the root of Oroxylum indicum, a Chinese medicinal plant. Based on 16S rRNA gene sequence analysis, strain D10(T) was a member of the genus Pseudonocardia and was most closely related, albeit loosely, to Pseudonocardia halophobica. Morphologi...
journal_title:International journal of systematic and evolutionary microbiology
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更新日期:2006-09-01 00:00:00
abstract::A starch-hydrolysing and extremely halophilic archaeon (strain BD-3(T)), isolated from Aibi salt lake in Xin-Jiang, China, was characterized phenotypically and genotypically in order to determine its taxonomic status. On the basis of its polar lipid composition, nucleotide sequences of its 16S rRNA genes, genomic DNA ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.64647-0
更新日期:2007-01-01 00:00:00
abstract::A yellow-coloured bacterial strain, designated BP-5(T), was isolated from a water sample of a cold spring located in the Hsinchu area of northern Taiwan. Cells of the strain were Gram-negative, non-spore-forming rods with a polar flagellum. The isolate was able to grow at 15-42 degrees C, 0-1 % NaCl and pH 6-8. The pr...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65755-0
更新日期:2008-10-01 00:00:00
abstract::A Gram-stain-positive, rod-shaped, yellow, non-motile, non-spore-forming, strictly aerobic bacterial strain, designated MW 10(T), was isolated from seawater of the Bay of Bengal, India, and was subjected to a polyphasic taxonomic study. Analysis of the 16S rRNA gene sequence revealed that strain MW 10(T) showed highes...
journal_title:International journal of systematic and evolutionary microbiology
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doi:10.1099/ijs.0.023184-0
更新日期:2011-08-01 00:00:00
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journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.030015-0
更新日期:2011-12-01 00:00:00
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journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-51-5-1633
更新日期:2001-09-01 00:00:00
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journal_title:International journal of systematic and evolutionary microbiology
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更新日期:2013-01-01 00:00:00
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journal_title:International journal of systematic and evolutionary microbiology
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更新日期:2017-11-01 00:00:00
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journal_title:International journal of systematic and evolutionary microbiology
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更新日期:2018-06-01 00:00:00
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journal_title:International journal of systematic and evolutionary microbiology
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更新日期:2021-01-05 00:00:00
abstract::A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterium, designated strain YLT18T, was isolated from mountain cliff soil of Enshi Grand Canyon in China. The major menaquinone was menaquinone 7 (MK-7) and the predominant fatty acids (>5 %) were iso-C15 : 0, C16 : 1ω5c, iso-C17 : 0 3-OH, summed feature 3 (C16 :...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.000566
更新日期:2015-11-01 00:00:00
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journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
abstract::In this study, strain P 461/12T, isolated from decaying plant material after mulching a pasture, was shown to represent a novel species of the genus Chryseobacterium by means of a polyphasic approach. The closest phylogenetic neighbours to the novel strain, as determined by 16S rRNA gene sequence analysis, were Chryse...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
abstract::Peatlands are important sources of CH(4) emissions to the atmosphere and molecular surveys have identified a diverse, but mainly uncultured, euryarchaeal community in them. Characterization of a strain, E1-9c(T), associated with uncultured group E1, from a minerotrophic fen is reported. Cells were regular cocci, usual...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.006890-0
更新日期:2009-05-01 00:00:00
abstract::A formal proposal is made to rename the order Haloanaerobiales as Halanaerobiales, the family Haloanaerobiaceae as Halanaerobiaceae, the genera Haloanaerobium and Haloanaerobacter as Halanaerobium and Halanaerobacter, respectively, and to change the specific epithets of Halanaerobium acetoethylicum, Halanaerobacter la...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-50-6-2229
更新日期:2000-11-01 00:00:00