Abstract：
:Metabolic conversion of homocysteine (Hcy) to a chemically reactive metabolite, Hcy-thiolactone, catalyzed by methionyl-tRNA synthetase is the first step in a pathway that is suggested to contribute to Hcy toxicity in humans. The accumulation of Hcy-thiolactone is detrimental because of its intrinsic ability to modify proteins by forming N-Hcy-protein adducts, in which a carboxyl group of Hcy is N-linked to the epsilon-amino group of a protein lysine residue. N-linked Hcy occurs in each protein examined and constitutes a significant pool of Hcy in the blood. N-Hcy proteins are likely to be recognized as neo-self antigens and induce an autoimmune response. Indeed, we found that autoantibodies specific for an Nepsilon-Hcy-Lys epitope on N-Hcy-proteins occur in humans. Serum levels of anti-N-Hcy-protein autoantibodies are directly correlated with plasma tHcy, but not with plasma cysteine or methionine levels. In a group of male patients with stroke, levels of anti-N-Hcy-protein autoantibodies and tHcy were significantly higher than in a group of healthy subjects. In a group of male patients with angiographically documented coronary artery disease, seropositivity for anti-N-Hcy-protein autoantibodies occurred five-fold more frequently than in controls and was an independent predictor of coronary artery disease. These findings show that the formation of N-Hcy-proteins has important physiological consequences and support a hypothesis that N-Hcy-protein is a neo-self antigen that contributes to immune activation, an important modulator of atherogenesis.
journal_name
Clin Chem Lab Medjournal_title
Clinical chemistry and laboratory medicineauthors
Jakubowski Hdoi
10.1515/CCLM.2005.177keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
1011-4issue
10eissn
1434-6621issn
1437-4331journal_volume
43pub_type
杂志文章,评审abstract：:Historical research on the concept of normality, its roots and its development show that this concept has its sources in very different areas of scientific and medical thinking. Of great importance were: (i) a new theory of disease arising early in the 19th century supposing a continuous change from the healthy to the...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 历史文章,杂志文章
doi：10.1515/CCLM.1998.013
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND:The plasma total and low-density lipoprotein-cholesterol (LDL-C) levels that are used as diagnostic criteria for familial hypercholesterolaemia (FH) probands in the general population are too stringent for use in relatives, given the higher prior probability of a first-degree relative being FH (50% vs. 1/500...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2008.135
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:The arginine derivatives asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) interfere with endothelial nitric oxide synthesis. Plasma ADMA and SDMA have been shown to be risk factors for cardiovascular disease and/or kidney function deterioration in a variety of patient populations. ME...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2007.277
更新日期：2007-01-01 00:00:00
abstract：BACKGROUND:Lactase persistence is an inherited autosomal dominant trait that confers the ability to digest lactose after weaning. Lactose persistence is caused by single nucleotide variants in a regulatory element for the lactase gene (LCT). In Europeans, lactase persistence is associated with the nucleotide variant LC...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/cclm-2014-0123
更新日期：2014-09-01 00:00:00
abstract：:It is well-established that more than 8% of patients examined for vitamin B12 deficiency unexpectedly have increased plasma levels of the vitamin, but so far there are no guidelines for the clinical interpretation of such findings. In this review, we summarise known associations between high plasma cobalamin and disea...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章,评审
doi：10.1515/cclm-2012-0545
更新日期：2013-03-01 00:00:00
abstract：:Although prostate-specific antigen (PSA), or human kallikrein 3, is the most valuable tool available for the diagnosis and management of prostate cancer, as currently used it is insufficiently sensitive and specific for early detection or staging of the malignancy. Many new concepts have been introduced in order to op...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章,评审
doi：10.1515/CCLM.1998.120
更新日期：1998-09-01 00:00:00
abstract：BACKGROUND:The Enhanced Liver Fibrosis (ELF) score combining serum hyaluronan, N-terminal peptide of type III procollagen and tissue inhibitor of metalloproteinase-1, was reported as relevant in predicting liver fibrosis in chronic liver disease and proposed as an alternative to liver biopsy. METHODS:We evaluated the ...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章,多中心研究
doi：10.1515/cclm-2011-0858
更新日期：2012-01-13 00:00:00
abstract：:The recent revision of ISO15189 has further strengthened its position as the standard for accreditation for medical laboratories. Both for laboratories and their customers it is important that the scope of such accreditation is clear. Therefore the European co-operation for accreditation (EA) demands that the national...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章,实务指引
doi：10.1515/cclm-2015-0257
更新日期：2015-07-01 00:00:00
abstract：:Quantification of serum proteins is useful in the diagnosis and clinical management of many disorders. With the introduction of automated analyzers and standardized reference materials, one of the last barriers to more widespread utilization of these measurements is the lack of availability of reliable and transferabl...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2001.180
更新日期：2001-11-01 00:00:00
abstract：:Objectives Our recent survey of 44 mass spectrometry laboratories across 17 countries identified variation in internal standard (IS) choice for the measurement of serum/plasma 17α-hydroxyprogesterone (17OHP) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The choice of IS may contribute to inter-method v...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/cclm-2020-0318
更新日期：2020-09-25 00:00:00
abstract：BACKGROUND:Angiotensin II is produced primarily by angiotensin I-converting enzyme (ACE) within atherosclerotic lesions and ACE level in plaques correlates with the severity of vessel wall damage. Therefore, we investigated the possible association of ACE gene insertion/deletion (I/D) polymorphism and the severity of a...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2008.101
更新日期：2008-01-01 00:00:00
abstract：:Small dense low-density lipoproteins (LDLs) have been associated with coronary heart disease (CHD) and type 2 diabetes in previous studies. However, the significance of small dense LDLs as a coronary risk factor for subjects with type 2 diabetes remains unclear. We measured mean LDL particle diameter by gradient gel e...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2005.076
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:Detection of systemic sclerosis-associated autoantibodies (SSc-Ab) is mostly restricted to anti-centromere and anti-topoisomerase-I. However, anti-RNA-polymerase-III and anti-PM/Scl are also important diagnostic markers for the disease supporting their incorporation in the laboratory repertoire. The aim of t...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/cclm-2013-0211
更新日期：2013-11-01 00:00:00
abstract：UNLABELLED:The pathogenesis of abdominal aortic aneurysms (AAA) is a complex process in which atherosclerosis and inflammation play a leading role. Cytokines are important mediators of both processes. The aim of our study was to determine whether plasma levels of cytokines which are most involved in AAA pathogenesis ca...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2000.178
更新日期：2000-11-01 00:00:00
abstract：:The application of turbidimetric homogeneous immunoassays made the determination of several plasma components widely available. The sensitivity and accuracy of these assays are appropriate enough for routine laboratory use; however, in the case of many pathologically high concentration samples, prozone effect (high do...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.1999.076
更新日期：1999-04-01 00:00:00
abstract：BACKGROUND:Sickle cell disease is a major health burden in India. The aim of the study was to compare the diagnostic utility of two different approaches on automated high performance liquid chromatography (HPLC) for newborn screening for sickle cell disorders and other haemoglobinopathies in India. METHODS:Newborn bab...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/cclm-2014-0452
更新日期：2014-12-01 00:00:00
abstract：:Until recently, the "human genome" programs were mainly directed towards the development of maps to identify disease genes. The genetic map comprises about 8000 highly informative second generation markers of the microsatellite type. The density of markers is now sufficient to localize a gene for a monogenic disease w...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.1998.086
更新日期：1998-08-01 00:00:00
abstract：BACKGROUND:Metabolic syndrome is closely related to several disturbances in lipid and lipoprotein metabolism. The aim of this study was to determine the association between apolipoprotein E (apoE) genotypes and the risk of metabolic syndrome and/or coronary heart disease complications. METHODS:The study included 279 s...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2007.258
更新日期：2007-01-01 00:00:00
abstract：BACKGROUND:Blood biomarkers are subject to pre-analytical variability. In many cases, the stability of important new tissue biomarkers during freeze cycles and storage has not been studied sufficiently. METHODS:To test the stability of matrix metalloproteinases-7 (MMP-7) and their tissue inhibitors (TIMP-1), vascular ...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2011.049
更新日期：2011-02-01 00:00:00
abstract：:Background The present study was undertaken to evaluate the clinical impact of a thyroglobulin (Tg) minirecovery test (Tg-mRec) in a large series of differentiated thyroid carcinoma (DTC) patients treated and monitored homogeneously in a tertiary referral center. Methods Included were 1120 serum samples from 798 DTC p...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/cclm-2018-1390
更新日期：2019-07-26 00:00:00
abstract：:Acute intestinal ischemic injury (i3) is a life-threatening condition with disastrous prognosis, which is currently difficult to diagnose at the early stages of the disease; a rapid diagnosis is mandatory to avoid irreversible ischemia, extensive bowel resection, sepsis and death. The overlapping protein expression of...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章,评审
doi：10.1515/cclm-2017-0291
更新日期：2018-02-23 00:00:00
abstract：BACKGROUND:External quality assessment/proficiency test (EQA/PT) organizers play an important role in monitoring the performance of HbA1c measurements. With increasing quality of the assays, HbA1c is increasingly used for diagnosis of diabetes and the demands on EQA/PT organizers themselves are rising constantly. EQA o...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/cclm-2016-0123
更新日期：2016-11-01 00:00:00
abstract：BACKGROUND:Pneumatic tube transport of blood samples reduces turnaround times and labour. However, the preanalytical effects on new clinical chemistry parameters and instruments are not fully known. The aim of this study was to evaluate the effect of pneumatic tube transport on haematology and coagulation parameters, i...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2008.288
更新日期：2008-01-01 00:00:00
abstract：:Increased expression of sialyl Lewis X or A antigens on metastatic cancer cells leads to their selectin-mediated extravasation. Profound fucosylation of the serum microenvironment may be a factor that interrupts adhesion and influences the formation of metastases. In this study we quantitatively analyzed fucosylation ...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2005.066
更新日期：2005-01-01 00:00:00
abstract：:In view of the role of pharmacotherapy in medicine, on the one hand, and the powerful technical possibilities that are now available on the other hand, therapeutic drug monitoring is a surprisingly neglected area of laboratory medicine. In this viewpoint article, an "omics approach" to pharmacovigilance and drug monit...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/cclm-2020-0339
更新日期：2020-05-05 00:00:00
abstract：BACKGROUND:Accurate reference intervals (RIs) based on a healthy pediatric population are essential for pediatric test result interpretation. The CALIPER project has recruited a large healthy cohort and completed a series of a priori studies to address gaps in pediatric RIs. As immunoassays from different manufacturers...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/cclm-2017-0349
更新日期：2018-01-26 00:00:00
abstract：BACKGROUND:Faecal immunochemical tests (FIT) are becoming widely used in colorectal cancer screening. Estimation of faecal haemoglobin concentration in a large group prompted an observational study on gender and age. METHODS:A single estimate of faecal haemoglobin concentration was made using quantitative automated im...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2011.815
更新日期：2011-12-07 00:00:00
abstract：:Background Intravenous lipid emulsion (ILE) is used to treat drug poisonings. The resultant hyperlipemia may affect laboratory tests but the consequences are poorly characterized. In a clinical trial we therefore investigated the effects of ILE on laboratory tests analyzed on common analytical platforms (Roche® cobas ...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章,随机对照试验
doi：10.1515/cclm-2018-0430
更新日期：2018-11-27 00:00:00
abstract：BACKGROUND:The stability limit of an analyte in a biological sample can be defined as the time required until a measured property acquires a bias higher than a defined specification. Many studies assessing stability and presenting recommendations of stability limits are available, but differences among them are frequen...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章,评审
doi：10.1515/cclm-2017-1189
更新日期：2018-10-25 00:00:00
abstract：:Since serum and plasma D-dimer concentrations correlate very well, we evaluated the comparability of other haemostasis activation markers in plasma and serum. Prothrombin fragment F1+2, fibrin monomer and D-dimer concentrations were measured with commercially available immunoassays in serum and plasma. Serum to plasma...
journal_title：Clinical chemistry and laboratory medicine
pub_type： 杂志文章
doi：10.1515/CCLM.2001.101
更新日期：2001-07-01 00:00:00
