Abstract：
:Histone deacetylase inhibitors (HDACi), which have emerged as a new class of anticancer agents, act by modulating expression of genes controlling apoptosis or cell proliferation. Here, we compared the effect of HDACi on transcriptional activation by estrogen or glucocorticoid receptors (ER and GR, respectively), two members of the steroid receptor family with cell growth regulatory properties. Like other transcription factors, steroid receptors modulate histone acetylation on target promoters. Using episomal reporter vectors containing minimal promoters to avoid promoter-specific effects, we observed that long-term (24-h) incubation with HDACi strongly stimulated GR-dependent but markedly repressed ER-dependent signaling in ER+/GR+ human endometrial carcinoma Ishikawa cells. These effects were reproduced on endogenous target genes and required incubation periods with HDACi substantially longer than necessary to increase global histone acetylation. Repression of estrogen signaling was due to direct inhibition of transcription from multiple ERalpha promoters and correlated with decreased histone acetylation of these promoters. In contrast, the strong HDACi stimulation of GR-dependent gene regulation was not accounted for by increased GR expression, but it was mimicked by overexpression of the histone acetyltransferase complex component transcriptional intermediary factor 2. Together, our results demonstrate striking and opposite effects of HDACi on ER and GR signaling that involve regulatory events independent of histone hyperacetylation on receptor target promoters.
journal_name
Mol Pharmacoljournal_title
Molecular pharmacologyauthors
Rocha W,Sanchez R,Deschênes J,Auger A,Hébert E,White JH,Mader Sdoi
10.1124/mol.105.014514keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
1852-62issue
6eissn
0026-895Xissn
1521-0111pii
mol.105.014514journal_volume
68pub_type
杂志文章abstract：:SR33557 belongs to a new class of molecules (indolizinsulfones) that act on the same receptor complex that has been characterized for other classical calcium channel effectors. The main binding properties of SR33557 to rabbit skeletal muscle are as follows. (i) Unlabeled SR33557 completely inhibits the specific bindin...
journal_title：Molecular pharmacology
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journal_title：Molecular pharmacology
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journal_title：Molecular pharmacology
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doi：
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journal_title：Molecular pharmacology
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journal_title：Molecular pharmacology
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doi：
更新日期：1991-07-01 00:00:00
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journal_title：Molecular pharmacology
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journal_title：Molecular pharmacology
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doi：
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journal_title：Molecular pharmacology
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doi：
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更新日期：1998-10-01 00:00:00
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journal_title：Molecular pharmacology
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更新日期：1996-11-01 00:00:00
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更新日期：1987-12-01 00:00:00
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journal_title：Molecular pharmacology
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doi：
更新日期：1991-04-01 00:00:00
