Abstract：
:Immunocytochemical detection of isolated tumor cells in peripheral blood and bone marrow is currently the most established method for monitoring early dissemination in epithelial cancer. In this study we used an immunomagnetic selection technique to develop an enrichment model for disseminated tumor cells in blood. Buffy coat cells spiked with varying numbers of BT-474 carcinoma cells were permeabilized and fixed, following which carcinoma cells were magnetically labelled with an anti-cytokeratin 8 mAb. Labelled cells were enriched by the use of magnetic columns. The eluted cytokeratin 8+ tumor cells were detected by flow cytometry and immunocytochemistry. Spiked samples were split and processed freshly in the immunomagnetic enrichment assay, as well as cryopreserved and processed in the assay after thawing. Enumeration of BT-474 cells demonstrated a detection limit of one BT-474 cell in 1.0 x 10(7) leukocytes in both fresh and cryopreserved-thawed samples. The pair wise comparison showed a significantly higher recovery of spiked BT-474 cells from freshly processed samples than from cryopreserved and thawed samples (57% vs 21%). Viability tests suggested that this outcome might be due to a greater susceptibility of BT-474 cells than buffy coat cells to the used cryopreservation and thawing technique. Altogether our findings show that the performance of the immunomagnetic enrichment assay on fresh samples is satisfactory with a recovery rate of almost 60% and a sensitivity of 10(-7). However, performance of the assay on cryopreserved and thawed cells needs to be improved.
journal_name
J Biol Regul Homeost Agentsjournal_title
Journal of biological regulators and homeostatic agentsauthors
Brooimans RA,de Leeuw N,Bontenbal M,Gratama JWkeywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
84-91issue
1-2eissn
0393-974Xissn
1724-6083journal_volume
19pub_type
杂志文章abstract：:Congenital melanocytic nevi are relatively rare lesions (1-6%). Their size and location can cause cosmetic and functional disorders of varying degrees of severity, but in addition they can also undergo transformation to malignant melanoma. Different types of treatments have been described as being effective for these ...
journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
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更新日期：2016-04-01 00:00:00
abstract：:Mast cells (MCs) are mediators of allergy and inflammation and participate in the growth of cancer cells. MCs can promote both neoangiogenesis and tumor growth. They increase in the stroma of certain tumors where they can be recruited by tumor-derived chemoattractants, such as monocyte chemotactic protein-1 (MCP-1), R...
journal_title：Journal of biological regulators and homeostatic agents
pub_type： 社论
doi：
更新日期：2016-10-01 00:00:00
abstract：:A high heterogeneity is found in the HIV-1 genome in vivo, not only between individuals, but also within a single individual. Different types of genetic heterogeneity of HIV-1 can be analyzed: the extension and the evolution of the viral quasispecies in blood, the variation between the virus obtained from different bo...
journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:Articular cartilage lesions represent a challenging problem for orthopaedic surgeons. The purpose of this study was to evaluate the effect of a new pulsed Nd:YAG High Intensity Laser Therapy on the regeneration of cartilage tissue in patients with traumatic lesions. Clinical, histological and immunohistochemical evalu...
journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
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更新日期：2012-10-01 00:00:00
abstract：:The use of minimally invasive surgical techniques undoubtedly represents a huge advantage for both the clinician and the patient. This case report shows the possibility of making an implant-prosthetic rehabilitation in an upper jaw in a patient who presented dental elements 1.3 and 2.3 in bone inclusion. The use of co...
journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
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更新日期：2017-04-01 00:00:00
abstract：: ...
journal_title：Journal of biological regulators and homeostatic agents
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更新日期：2019-01-01 00:00:00
abstract：:Minoxidil is the only US FDA-approved topical drug for the treatment of female and male pattern hair loss. Previously, it was demonstrated that topical minoxidil is metabolized to its active metabolite, minoxidil sulfate, by sulfotransferase enzymes located in the outer root sheath of hair follicles. The expression of...
journal_title：Journal of biological regulators and homeostatic agents
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更新日期：2018-07-01 00:00:00
abstract：:Bisphosphonates are antiresorptive pharmacological agents used in the treatment of osteoporosis. Recently, osteonecrosis of the jaw has been recognized as a potential side effect in patients on long-term bisphosphonate therapy. This condition, popularly called bisphosphonate-related osteonecrosis of the jaw (BRONJ), h...
journal_title：Journal of biological regulators and homeostatic agents
pub_type： 临床试验,杂志文章
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更新日期：2017-07-01 00:00:00
abstract：:Cerebral small vessel disease (CSVD) is a leading cause of progressive decline of cognition and a major risk factor for stroke. Thymoquinone (TQ) is the major biological component of Nigella sativa (N. sativa) and its extracts. We explored the possible protective effect of TQ against CSVD in strokeprone spontaneously ...
journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：2018-03-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 信件
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更新日期：2020-03-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 社论,评审
doi：
更新日期：2009-07-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 信件
doi：
更新日期：2019-05-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 信件
doi：
更新日期：2018-11-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：2006-07-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 信件
doi：
更新日期：2008-10-01 00:00:00
abstract：:To investigate the role played by chemokines in the natural history of human immunodeficiency virus (HIV) infection, we measured the plasma levels of RANTES. MIP-1 alpha and MIP-1 beta in a cohort of patients with primary HIV-1 infection (PHI) followed longitudinally. The cohort included 17 patients with well-document...
journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Despite a wide range of available antidepressants, the effect of the treatment is often suboptimal and there is a need for more effective and better tolerated drugs. Unlike other antidepressants, agomelatine represents a new approach to depression with an innovative mechanism of action. It is an agonist of melatoniner...
journal_title：Journal of biological regulators and homeostatic agents
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：2011-01-01 00:00:00
abstract：:Upper respiratory infections are widespread in clinical practice. Antibiotics are frequently used in the management of patients with airways infection. However, antibiotics can induce intestinal and respiratory dysbiosis that, in turn, worsens respiratory symptoms. Moreover, respiratory infections per se can cause dys...
journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章,评审
doi：
更新日期：2020-11-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 信件
doi：
更新日期：2014-10-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 信件,多中心研究,随机对照试验
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更新日期：2018-03-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 信件,随机对照试验
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更新日期：2016-10-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 社论
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章,随机对照试验
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更新日期：2016-01-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 杂志文章
doi：
更新日期：2020-01-01 00:00:00
