Abstract:
:Although histone deacetylase (HDAC) inhibitors are emerging as a promising new treatment strategy in malignancy, how they exert their effect on human non-small cell lung cancer cells is as yet unclear. This study was undertaken to investigate the underlying mechanism of an HDAC inhibitor, Trichostatin A (TSA), -induced apoptosis in a human lung carcinoma cell line A549. The effects of this compound were also tested on cyclooxygenase (COX) activity. Treatment of A549 cells to TSA resulted in the inhibition of viability and the induction of apoptosis in a concentration-dependent manner, which could be proved by trypan blue counts, DAPI staining, agarose gel electrophoresis and flow cytometry analysis. Apoptosis of A549 cells by TSA was associated with a down-regulation of anti-apoptotic Bcl-2 protein and an up-regulation of pro-apoptotic Bax protein. TSA treatment induced the proteolytic activation of caspase-3 and caspase-9, and a concomitant degradation of poly(ADP-ribose)-polymerase protein. Furthermore, TSA decreased the levels of COX-2 mRNA and protein expression without significant changes in the levels of COX-1, which was correlated with an inhibition in prostaglandin E2 synthesis. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of TSA.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Choi YHkeywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
473-9issue
2eissn
1019-6439issn
1791-2423journal_volume
27pub_type
杂志文章abstract::Structural maintenance of chromosome 1 alpha (SMC1A) gene has been reported to be related to tumor development in some types of human cancers. However, the misregulation of SMC1A and its functions in castration-resistant prostate cancer (CRPC) have not been well understood. In the present study, we found that SMC1A wa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3697
更新日期:2016-11-01 00:00:00
abstract::Breast cancer (BC) is the most frequently malignancy in women. Therefore, establishment of an animal model for the development of preventative measures and effective treatment for tumors is required. A novel heterogeneous spontaneous mammary tumor animal model of Kunming mice was generated. The purpose of this study w...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2657
更新日期:2014-12-01 00:00:00
abstract::Regulatory T cells (CD4+CD25+FoxP3+, Treg) have been shown to play a major role in suppression of the immune response to malignant gliomas. In this study, we investigated the kinetics of Treg infiltration in metastatic brain tumor models, including melanoma, breast and colon cancers. Our data indicate that both CD4+ a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000282
更新日期:2009-06-01 00:00:00
abstract::Liquid crystals are the state of matter existing between liquid and crystalline phases, and recently there has been increasing interest in their biological effects. Following our recently reported work, we investigated the cell suppressive effects of liquid crystal-related compounds (LCRCs), which are precursors of li...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1804
更新日期:2013-04-01 00:00:00
abstract::Resistance to radiotherapy and chemotherapy currently represents one of the major reasons for therapeutic failure in nasopharyngeal carcinoma (NPC). However, the mechanisms underlying resistance to chemotherapy in NPC remain unclear. In this study, cell counting assay, cell cycle assay and senescence associated β-gala...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4462
更新日期:2018-09-01 00:00:00
abstract::Patients with pancreatic cancer frequently demonstrate symptoms such as weight-loss and muscle wasting and have clinical evidence of a systemic inflammatory response. Such effects may be mediated by pro-inflammatory cytokines derived from tumor cells. The production of interleukin-6 and -8 by pancreatic cancer cell li...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.21.4.881
更新日期:2002-10-01 00:00:00
abstract::Invasiveness and metastatic potential are the two most important properties defining malignancy. The adeno-virus E1A (Ad-E1A) gene has a dual effect as a proliferative gene and as a tumor-suppressor gene, decreasing tumor growth and the metastatic potential of malignant cells. In order to study genes related with the ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::A total of forty-one tumor infiltrating T cell lines (TIL) were cloned, in the presence of interleukin-2, from nine breast tumor and five melanoma specimens with limiting dilution in a microculture system. Nineteen (46%) of the lines/clones reacted to autologous tumor targets. The T cell receptor (TcR) V beta gene usa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.1.147
更新日期:1995-07-01 00:00:00
abstract::VEGF, Hedgehog, FGF, Notch, and WNT signaling pathways network together for vascular remodeling during embryogenesis, tissue regeneration, and carcinogenesis. VEGFA (VEGF), VEGFB, VEGFC, VEGFD (FIGF) and PGF (PlGF) are VEGF family ligands for receptor tyrosine kinases, including VEGFR1 (FLT1), VEGFR2 (KDR) and VEGFR3 ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract::Cancer cells genetically modified to secrete immunoregulatory cytokines offer great promise for human cancer treatment as tumor vaccines. However, in preclinical animal studies, large established cancer burdens have appeared difficult to eradicate with such vaccines. For example, lethally-irradiated GM-CSF-secreting C...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.12.1.161
更新日期:1998-01-01 00:00:00
abstract::The Stockholm Central Railway Station in Sweden was investigated for public radiofrequency (RF) radiation exposure. The exposimeter EME Spy 200 was used to collect the RF exposure data across the railway station. The exposimeter covers 20 different radiofrequency bands from 88 to 5,850 MHz. In total 1,669 data points ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3657
更新日期:2016-10-01 00:00:00
abstract::Cytogenetic analyses of peripheral blood cells of breast cancer patients unveiled in one case a grossly enlarged G-band in one copy of chromosome 16 (16qh+), which scored also strongly positive in C-banding. Fluorescence in situ hybridization with both a total chromosome 16 library and with probe pHuR195 detecting loc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.2.347
更新日期:1994-02-01 00:00:00
abstract::Gene amplification is an important phenomenon in the pathogenetics of malignancy. A method is defined which allows the determination of gene amplification from any sample from which RNA can be obtained, and requires no a priori knowledge of the cytogenetic or genomic state of the tumor. This method allows the determin...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::Golgi phosphoprotein 2 (GP73) is a type II Golgi protein, which was found on examination of the fucosylated proteome as a potential tumor marker for hepatocellular carcinoma (HCC). The serum levels of both total and fucosylated GP73 were increased in the sera of patients with HCC. Fucosylation is one of the most impor...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1005
更新日期:2011-07-01 00:00:00
abstract::The effects of different substrate stiffness were investigated on epithelial-mesenchymal transition (EMT) of cervical cancer cell lines and the role of miR-106b and its target protein DAB2 therein. Cervical cancer cell lines HeLa and SiHa were cultured on artificial substrates with different stiffness prepared using d...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3978
更新日期:2017-06-01 00:00:00
abstract::The impacts of post‑operative abdominal infectious complications increase hematogenous distant metastasis and result in poor long‑term survival after curative resection. Even if curative resection can be performed, the presence of circulating tumor cells is affected. The liver, the most common site of metastases, is a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5102
更新日期:2020-10-01 00:00:00
abstract::Neuroblastoma, originated from neural crest cells, is the most common extracranial solid tumor in childhood. In the present study, we evaluated in vitro the oncolytic effect of live-attenuated poliovirus on human neuroblastoma cell lines, and in vivo its therapeutic efficacy in human neuroblastoma-bearing athymic mice...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Tetramethypyrazine (TMP), one of the active compounds extracted from the traditional Chinese medicinal herb (Chuanxiong), has been verified as an anticancer compound against several types of cancer. However, understanding of the molecular mechanisms have not been fully elucidated. In the present study, the anticancer ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3670
更新日期:2016-10-01 00:00:00
abstract::No population-based study has been conducted on the relationship between chemotherapy and the risk of cognitive impairments in patients with colorectal cancer. This study aimed to determine this association in a large population-based cohort of patients. We studied 72,374 men and women who were diagnosed with stages I...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1882
更新日期:2013-06-01 00:00:00
abstract::Estrogens act through interactions with estrogen receptors (ERs) to play diverse roles in various pathophysiological conditions. A number of synthetic selective estrogen receptor modulators (SERMs), such as tamoxifen and raloxifene, have been developed and used to treat ER-related diseases, including breast cancer and...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3002
更新日期:2015-07-01 00:00:00
abstract::Autocrine fibroblast growth factor (FGF) signaling was genetically manipulated in human gliomas by an adenovirus-mediated strategy. Antisense inhibition of endogenous basic FGF (bFGF) expression showed inhibition of the proliferation of human glioma cells (U251MG, NMC-G1) without promoting apoptosis. The reduction in ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::We recently demonstrated lymphoma development in transgenic mice deficient in retinoic acid receptor alpha (RARalpha). High incidence of lymphoma development in this transgenic mouse model system was similar to lymphoma development in p53 knockout mice. In an effort to understand the molecular basis of lymphomagenesis...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.16.3.561
更新日期:2000-03-01 00:00:00
abstract::CD26 is a surface glycoprotein with intrinsic dipeptidyl peptidase IV (DPPIV) enzyme activity with multiple biological roles, including being intricately involved in immunoregulation as a T-cell activation molecule and as a regulator of chemokine function. T-cell lymphoid malignancies represent a heterogeneous group o...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2003-03-01 00:00:00
abstract::Breast cancer cells can switch from estrogen receptor α (ER)- to human epidermal growth factor receptor (HER)-driven cell growth upon acquiring antiestrogen resistance. HER ligands are cleaved by metalloproteinases leading to release of active HER ligands, activation of HER receptors and consequently increased cell gr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2434
更新日期:2014-07-01 00:00:00
abstract::Previously, we found that the injection of zoledronic acid (ZOL) into mice bearing tumor induced changes of the vascular structure in the tumor. In this study, we examined whether ZOL treatment could decrease interstitial fluid pressure (IFP) via change of tumor vasculature, and enhance the antitumor efficacy of lipos...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2991
更新日期:2015-07-01 00:00:00
abstract::The efficacy of radioimmunotherapy with radiolabelled monoclonal antibody (Mab) depends on the amount of antibody taken up by the tumour and on its intratumoral distribution. Multicellular spheroids (MTS) of lung cell carcinoma was investigated to study cellular and subcellular distribution of a Mab Po66 labelled with...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.3.609
更新日期:1997-03-01 00:00:00
abstract::p57KIP2, the second class of KIP family protein, is one of the negative regulators of the cell cycle. To elucidate the role of p57KIP2 in colorectal normal mucosa and cancer, we examined the expression of p57KIP2 protein in 110 pairs of colorectal non-tumor and cancer tissues. Immunohistochemical analysis showed that ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.19.1.39
更新日期:2001-07-01 00:00:00
abstract::Most solid tumors display extracellular acidosis, which only partially overlaps with hypoxia and induces distinct adaptive changes leading to aggressive phenotype. Although acidosis is mainly attributable to excessive production of lactic acid, it also involves carbonic anhydrase (CA) IX-mediated conversion of CO(2) t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-10-01 00:00:00
abstract::Bile duct carcinoma patients generally have a poor prognosis. Understanding this cancer at the biological, genetic, molecular, and cellular level in ways relevant to clinical management is essential for developing effective preventive and therapeutic regimens. However, the currently established bile duct cancer cell l...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-02-01 00:00:00
abstract::Lung cancer is the most common and most lethal type of cancer. A sustained proliferative capacity is one of the hallmarks of cancer, and microtubules serve an important role in maintaining a sustained cell cycle. Therefore, understanding the regulation of microtubule proteins in the cell cycle is important for tumor p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4805
更新日期:2019-07-01 00:00:00