Effect of colchicine on ganglioside composition of rat primary culture neurons.

Abstract:

:Developmental changes in gangliosides in the course of neurite outgrowth were examined in dissociated fetal rat cerebral neurons in culture. About a 2-fold increase in ganglioside levels was seen with the progression of neurite formation for up to 24 h in predominantly neuronal cultures. Ganglioside patterns appeared to be unchanged during the first 24 h, subsequently consisted of higher amounts of GD3 and b-series gangliosides (such as GD1b, GT1b, and GQ1b), and lower amounts of a-series gangliosides (GM1 and GD1a). Although the addition of colchicine to the cell growth medium inhibited neurite outgrowth in developing neurons, little if any differences in ganglioside patterns were found between control and colchicine-treated cells. Ganglioside levels decreased slightly in colchicine-treated cells in agreement with the decrease in cell attachment to culture dishes. Although colchicine treatment 8 h after plating caused complete retraction of formed neurites, the ganglioside level of the cells continued to increase during the following 16-hour incubation. Thus, the data suggest that ganglioside synthesis in differentiating neurons does not primarily accompany the expansion in cell surfaces due to neurite formation, and raises the possibility that a large proportion of gangliosides is retained in intracellular compartments.

journal_name

Dev Neurosci

authors

Ogiso M,Ohta M,Harada Y,Hirano S

doi

10.1159/000111647

keywords:

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

53-60

issue

1

eissn

0378-5866

issn

1421-9859

journal_volume

14

pub_type

杂志文章
  • Ontogeny of insulin binding in different regions of the rat brain.

    abstract::Insulin binding was measured on crude mitochondrial or plasma membranes prepared from different rat brain regions during postnatal development. In the cerebral cortex and brainstem, insulin binding decreased 60-70% between birth and the adult period. In the cerebellum, insulin binding doubled in the first 10 postnatal...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000111863

    authors: Marks JL,Eastman CJ

    更新日期:1990-01-01 00:00:00

  • Lethal giant puzzle of Lgl.

    abstract::Cell polarity is one of the most basic properties of all normal cells and loss of polarity is a hallmark of cancer. While multiple proteins have been implicated in the maintenance of cell polarity, the functionally related neoplastic tumor suppressors Lethal giant larvae (Lgl), Scribble and Disks large comprise a uniq...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章,评审

    doi:10.1159/000090749

    authors: Vasioukhin V

    更新日期:2006-01-01 00:00:00

  • Expression of cell surface markers and myelin proteins in cultured oligodendrocytes from neonatal brain of rat and mouse: a comparative study.

    abstract::Dissociated brain cell cultures are a useful model for investigating development and differentiation of oligodendrocytes in vitro. The current studies compare the developmental patterns of expression for oligodendrocyte lineage/myelin markers in both primary and secondary oligodendrocyte cultures derived from mouse an...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000111276

    authors: Duchala CS,Asotra K,Macklin WB

    更新日期:1995-01-01 00:00:00

  • 'Hot spots' of creatine kinase localization in brain: cerebellum, hippocampus and choroid plexus.

    abstract::Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellum extracts analyzed by two-dimensional gels, using antipeptide antibodies specific for sarcomeric muscle-type mitochondrial CK (Mib-CK) and revealed the presence o...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章,评审

    doi:10.1159/000111452

    authors: Kaldis P,Hemmer W,Zanolla E,Holtzman D,Wallimann T

    更新日期:1996-01-01 00:00:00

  • Is being plastic fantastic? Mechanisms of altered plasticity after developmental traumatic brain injury.

    abstract::Traumatic brain injury (TBI) is predominantly a clinical problem of young persons, resulting in chronic cognitive and behavioral deficits. Specifically, the physiological response to a diffuse biomechanical injury in a maturing brain can clearly alter normal neuroplasticity. To properly evaluate and investigate develo...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章,评审

    doi:10.1159/000094163

    authors: Giza CC,Prins ML

    更新日期:2006-01-01 00:00:00

  • Regionally specific effects of BDNF on oligodendrocytes.

    abstract::To define the effects of neurotrophins on oligodendrocytes, we monitored NGF, BDNF and NT-3 actions on basal forebrain (BF) and cortical populations. NGF, BDNF and NT-3 applied to BF oligodendrocytes elicited increases in expression of myelin basic protein (MBP) and enhanced the numbers of MBP+ cells, without affectin...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000072261

    authors: Du Y,Fischer TZ,Lee LN,Lercher LD,Dreyfus CF

    更新日期:2003-03-01 00:00:00

  • Striatal neuroprotection from neonatal hypoxia-ischemia in piglets by antioxidant treatment with EUK-134 or edaravone.

    abstract::Striatal neurons are highly vulnerable to hypoxia-ischemia (HI) in term newborns. In a piglet model of HI, striatal neurons develop oxidative stress and organelle disruption by 3-6 h of recovery and ischemic cytopathology over 6-24 h of recovery. We tested the hypothesis that early treatment with the antioxidants EUK-...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000327243

    authors: Ni X,Yang ZJ,Carter EL,Martin LJ,Koehler RC

    更新日期:2011-01-01 00:00:00

  • Evidence for regulation of myelin protein synthesis by contact between adjacent Schwann cell plasma membranes.

    abstract::The spontaneously immortalized S16 Schwann cell line expresses higher levels of myelin-associated glycoprotein (MAG) and PO glycoprotein and their messenger RNAs when grown at high density than at low density [Sasagasako et al: J Neurochem 1996;66:1432-1439]. This up-regulation of myelin protein expression at high den...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000017409

    authors: Sasagasako N,Ohno M,Quarles RH

    更新日期:1999-01-01 00:00:00

  • Differential expression of Na,K-ATPase isoforms in oligodendrocytes and astrocytes.

    abstract::Using Western blot and immunocytochemistry to detect protein, and in situ hybridization and Northern blot to detect RNA, we studied the expression of Na,K-ATPase subunit isoforms in cultured astrocytes and oligodendrocytes in vitro, and in glial cells in the brain. We found that astrocytes in culture express alpha 1 a...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000111422

    authors: Fink D,Knapp PE,Mata M

    更新日期:1996-01-01 00:00:00

  • Role of mitochondria in neuronal apoptosis.

    abstract::Apoptosis is a controlled form of cell death that participates in the demise of neuronal cells during development, neurodegenerative disorders and exposure to neurotoxic agents. In recent years, the mitochondria have emerged as being pivotal in controlling apoptosis. They house a number of apoptogenic molecules that a...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章,评审

    doi:10.1159/000017460

    authors: Gorman AM,Ceccatelli S,Orrenius S

    更新日期:2000-09-01 00:00:00

  • Inhibition of nitric oxide synthase fails to disrupt the development of cholinergic fiber patches in the rat superior colliculus.

    abstract::Nitric oxide may serve as a retrograde messenger to refine or stabilize synapses in the developing nervous system. Whether this action is dependent upon glutamate and the N-methyl-D-aspartate receptor is not yet established. We have used the patch-cluster system in the intermediate gray layer (IGL) of the rat superior...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000111215

    authors: Mize RR,Scheiner CA,Salvatore MF,Cork RJ

    更新日期:1997-01-01 00:00:00

  • Antisense studies in PC12 cells suggest a role for H218, a sphingosine 1-phosphate receptor, in growth-factor-induced cell-cell interaction and neurite outgrowth.

    abstract::Our previous studies of H218, a sphingosine 1-phosphate (S1P) receptor and a member of the G-protein-coupled receptor superfamily, suggest that it may participate in mammalian nervous system development. Thus, brain levels of H218 mRNA are higher during early neurogenesis than postnatally. In addition, embryonic H218 ...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000017452

    authors: MacLennan AJ,Devlin BK,Marks L,Gaskin AA,Neitzel KL,Lee N

    更新日期:2000-01-01 00:00:00

  • Characterization of endothelial cell transport in the developing mouse blood-brain barrier.

    abstract::The cerebromicrovasculature of immature mice ranging in age from 1-24 days after birth was studied by electron microscopy. A micromanipulator apparatus enabled us to facilitate direction of a needle cannula and injection into leg veins or hearts with horseradish peroxidase (HRP) or ferritin (anionic and cationic) trac...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000112242

    authors: Lossinsky AS,Vorbrodt AW,Wisniewski HM

    更新日期:1986-01-01 00:00:00

  • Investigation of the changes in neuronal distribution and phosphorylation state of MAP1X during development.

    abstract::Neurite outgrowth is dependent on the presence in neurites of assembled microtubules which consist of polymerized tubulin to which microtubule-associated proteins (MAPs) are bound. This study adds to previous evidence of the similarity between the high molecular weight MAPs, MAP1X, MAP1B and MAP5, and explains the dif...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000111318

    authors: Harrison L,Cheetham ME,Calvert RA

    更新日期:1993-01-01 00:00:00

  • Chronic neurological deficits in mice after perinatal hypoxia and ischemia correlate with hemispheric tissue loss and white matter injury detected by MRI.

    abstract::We investigated the effects of perinatal hypoxia-ischemia (HI) on brain injury and neurological functional outcome at postnatal day (P)30 through P90. HI was induced by exposing P9 mice to 8% O(2) for 55 min using the Vannucci HI model. Following HI, mice were treated with either vehicle control or Na(+)/H(+) exchange...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000328430

    authors: Cengiz P,Uluc K,Kendigelen P,Akture E,Hutchinson E,Song C,Zhang L,Lee J,Budoff GE,Meyerand E,Sun D,Ferrazzano P

    更新日期:2011-01-01 00:00:00

  • Ischemic-Reperfusion Injury Increases Matrix Metalloproteinases and Tissue Metalloproteinase Inhibitors in Fetal Sheep Brain.

    abstract::Hypoxic-ischemic brain injury is a leading cause of neurodevelopmental morbidities in preterm and full-term infants. Blood-brain barrier dysfunction represents an important component of perinatal hypoxic-ischemic brain injury. The extracellular matrix (ECM) is a vital component of the blood-brain barrier. Matrix metal...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000489700

    authors: Chen X,Patra A,Sadowska GB,Stonestreet BS

    更新日期:2018-01-01 00:00:00

  • Self-biting induced by activation of L-type calcium channels in mice: serotonergic influences.

    abstract::The L-type calcium channel activator +/-Bay K 8644 has recently been shown to provoke self-injurious biting in young mice. Since the serotonergic systems have been implicated in the expression of self-injurious behavior in both humans and animals, the present studies tested whether drugs influencing serotonin systems ...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000066747

    authors: Kasim S,Egami K,Jinnah HA

    更新日期:2002-01-01 00:00:00

  • In vivo Monitoring of Cerebral Hemodynamics in the Immature Rat: Effects of Hypoxia-Ischemia and Hypothermia.

    abstract:BACKGROUND:Neonatal hypoxic-ischemic (HI) encephalopathy occurs in 1-4 per 1,000 live term births and can cause devastating neurodevelopmental disabilities. Currently, therapeutic hypothermia (TH) is the only treatment with proven efficacy. Since TH is associated with decreased cerebral metabolism and cerebral blood fl...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000381704

    authors: Buckley EM,Patel SD,Miller BF,Franceschini MA,Vannucci SJ

    更新日期:2015-01-01 00:00:00

  • Perinatal hypoxic-ischemic brain damage: evolution of an animal model.

    abstract::Early research in the Vannucci laboratory prior to 1981 focused largely on brain energy metabolism in the developing rat. At that time, there was no experimental model to study the effects of perinatal hypoxia-ischemia in the rodent, despite the tremendous need to investigate the pathophysiology of perinatal asphyxial...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章,评审

    doi:10.1159/000085978

    authors: Vannucci RC,Vannucci SJ

    更新日期:2005-03-01 00:00:00

  • Brain Barrier Disruption and Region-Specific Neuronal Degeneration during Necrotizing Enterocolitis in Preterm Pigs.

    abstract::Necrotizing enterocolitis (NEC) increases the risk of brain injury and impaired neurodevelopment. Rapid brain maturation prior to birth may explain why preterm brains are particularly vulnerable to serious infections. Using pigs as models, we hypothesized that preterm birth was associated with altered blood-cerebrospi...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000488979

    authors: Brunse A,Abbaspour A,Sangild PT

    更新日期:2018-01-01 00:00:00

  • Shifts in hormonal stress reactivity during adolescence are not associated with changes in glucocorticoid receptor levels in the brain and pituitary of male rats.

    abstract::Preadolescent animals display protracted hormonal stress responses mediated by the hypothalamic-pituitary-adrenal (HPA) axis compared to adults. Though the mechanisms that underlie this shift in stress reactivity are unknown, reduced glucocorticoid-dependent negative feedback on the HPA axis has been posited to contri...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000362873

    authors: Dziedzic N,Ho A,Adabi B,Foilb AR,Romeo RD

    更新日期:2014-01-01 00:00:00

  • Brain creatine kinase and creatine transporter proteins in normal and creatine-treated rabbit pups.

    abstract::Systemic creatine (Cr) supplementation increases brain phosphocreatine (PCr) and prevents hypoxic seizures in 15-day-old rabbits. Between 5 and 30 days of age during normal development, rabbit gray matter mitochondrial creatine kinase (Mi-CK) increases 400% while cytosolic CK (BB-CK) increases 60%. In white matter, bo...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000017473

    authors: Kekelidze T,Khait I,Togliatti A,Holtzman D

    更新日期:2000-09-01 00:00:00

  • Pituicyte fine structure in the developing neural lobe of the rat.

    abstract::The fine structure of pituicytes was investigated between 15 days of fetal life and 120 days postnatum. Prior to the penetration of neurosecretory axons into the neural lobe a uniform population of undifferentiated pituicytes is presented. Coincidental with the first appearance of neurosecretory axons in the neural lo...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000112744

    authors: Dellmann HD,Sikora K

    更新日期:1981-01-01 00:00:00

  • Relation between cerebral blood flow and extracellular glucose in rat striatum during mild hypoxia and hyperoxia.

    abstract::Rats were exposed to mild hyperoxia and hypoxia by the administration of oxygen/air and nitrogen/air mixtures through plastic tubing held close to their snouts for periods of 3 min. Changes in tissue oxygen were monitored at an implanted carbon paste electrode; local cerebral blood flow (rCBF) at an implanted platinum...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000017298

    authors: Lowry JP,Demestre M,Fillenz M

    更新日期:1998-01-01 00:00:00

  • GABA, hypotaurine and taurine transport in brain slices from developing mouse.

    abstract::The kinetics and sodium-dependence of gamma-aminobutyric acid (GABA), hypotaurine and taurine uptake were analyzed in cerebral slices prepared from mice of different postnatal ages. The maximal velocities of transport (V) and the Km constants for the high-affinity uptake all decreased with age, whereas the development...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000112354

    authors: Oja SS,Kontro P

    更新日期:1983-01-01 00:00:00

  • Ciliary neurotrophic factor reduces the proliferation and promotes the differentiation of TH- MYCN transformed sympathoadrenal progenitors.

    abstract::Neuroblastoma is a childhood cancer caused by the transformation of sympathoadrenal progenitors. By following the formation of tumors in homozygous TH-MYCN mice, an established mouse model of neuroblastoma, we were able to capture transformed cells prior to the formation of large, vascularized tumors in order to deter...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000365281

    authors: DeWitt J,Pappas A,Nishi R

    更新日期:2014-01-01 00:00:00

  • Microtubule proteins in PC12 pheochromocytoma cells. Isolation, composition and in vitro phosphorylation.

    abstract::In the presence of nerve growth factor (NGF) PC12 pheochromocytoma cells develop properties of sympathetic neurons and extend long microtubule-containing neurites. PC12 cell microtubule protein was isolated using an assembly and disassembly procedure, either directly from an unlabeled cell extract or by copolymerizati...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000112330

    authors: Richter-Landsberg C,Landreth GE,Shooter EM

    更新日期:1983-01-01 00:00:00

  • Age and etiology as predictors of language outcome following hemispherectomy.

    abstract::We report on the effects of etiology and age on the linguistic outcomes in a large pediatric hemispherectomy population. Four populations were considered separately: cortical dysplasia (multilobar involvement), Rasmussen's encephalitis, infarction as a primary etiology and, fourth, children who failed to develop langu...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000017396

    authors: Curtiss S,de Bode S

    更新日期:1999-11-01 00:00:00

  • Synapse formation is related to the onset of neuron-specific enolase immunoreactivity in the avian auditory and vestibular systems.

    abstract::Neuron-specific enolase (NSE) is a unique form of the glycolytic enzyme, enolase, which, in the brain, is strictly localized in differentiated neurons and neuroendocrine cells. In this report evidence is presented that NSE is first seen in the cell bodies of developing chick auditory and vestibular neurons shortly aft...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000112689

    authors: Whitehead MC,Marangos PJ,Connolly SM,Morest DK

    更新日期:1982-01-01 00:00:00

  • Different Developmental Pattern of Brain Activities in ADHD: A Study of Resting-State fMRI.

    abstract::There are distinct symptoms for attention deficit hyperactivity disorder (ADHD) at different ages. To explore the developmental mechanism of ADHD from childhood to adolescence, patients from different age groups with ADHD drawn from a large dataset should be investigated. In this study, we hypothesized that there are ...

    journal_title:Developmental neuroscience

    pub_type: 杂志文章

    doi:10.1159/000490289

    authors: Tang C,Wei Y,Zhao J,Nie J

    更新日期:2018-01-01 00:00:00