Abstract：
:Melanoma-associated germline mutations affecting the tumor suppressor and cyclin-dependent kinase (CDK) inhibitor, CDKN2A/p16INK4a, have been identified in over 100 melanoma-prone families worldwide. To predict the melanoma risk for carriers of specific mutations, mutant p16INK4a can be tested in biochemical and cellular assays. In most cases, p16INK4a mutations with predicted disease relation, due to segregation with melanoma, are functionally impaired in such assays. The N-terminal 24 base pair duplication of CDKN2A, however, encodes a p16INK4a variant previously shown to have wild-type function, despite segregating with melanoma in at least 5 melanoma families. To clarify whether the duplication mutation has a cell cycle regulatory defect or behaves like wild-type p16INK4a, we reanalyzed the cell cycle-inhibitory activity of this mutation. Stable cell clones of the p16-null WMM1175 melanoma cell line inducible for ectopic p16INK4a were used in this study. In these cells, p16INK4a expression can be controlled at physiologic levels. Our results show that in comparison to wild-type p16INK4a, the duplication mutant induced weaker S-phase inhibition and cells expressing this mutant form of p16INK4a retained colony formation ability. We also show that the cell cycle-regulatory defect of the p16INK4a duplication mutant was associated with decreased inhibition of pRb phosphorylation even though it retained significant binding to CDK4.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Becker TM,Ayub AL,Kefford RF,Mann GJ,Rizos Hdoi
10.1002/ijc.21265keywords:
subject
Has Abstractpub_date
2005-11-20 00:00:00pages
569-73issue
4eissn
0020-7136issn
1097-0215journal_volume
117pub_type
杂志文章abstract：:Conversion of solid sarcomas and carcinomas into ascites tumors depends on the in vivo selection of phenotypically altered tumor cell variants that can grow in the dissociated form. Once selected, they retain this property even after prolonged s.c. growth as solid tumors. From an s.c.-passaged subline of an ascites-co...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910450324
更新日期：1990-03-15 00:00:00
abstract：:Diabetes refers to a group of metabolic diseases characterized by impaired insulin signalling and high blood glucose. A growing body of epidemiological evidence links diabetes to several types of cancer but the underlying molecular mechanisms are poorly understood. The signalling cascade connecting insulin and FOXO pr...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.30840
更新日期：2017-12-15 00:00:00
abstract：:HER2 is an erbB/HER type 1 tyrosine kinase receptor that is frequently over-expressed in malignant epithelial tumours. Herceptin, a humanised mouse monoclonal antibody to HER2, is proven therapeutically in the management of metastatic breast cancer, significantly prolonging survival when combined with cytotoxic chemot...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.1214
更新日期：2001-05-15 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) is a major inducer of angiogenesis in tumors. Basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) have both been shown to interact with VEGF. The involvement of VEGF, bFGF, and EGF in melanoma angiogenesis was investigated here. Four human melanoma cell lin...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19980610)76:6<836::aid-ijc
更新日期：1998-06-10 00:00:00
abstract：:We have previously identified a region containing 16 CpGs within the MGMT CpG islands which is critical for the transcriptional control of MGMT (Malley, Acta Neuropathol 2011). To investigate the patterns and incidence of MGMT methylation in astrocytic and oligodendroglial tumors, we quantitatively assessed methylatio...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.26499
更新日期：2012-09-01 00:00:00
abstract：:Renewed interest in the use of therapeutic enzymes combined with an improved knowledge of cancer cell metabolism, has led to the translation of several arginine depletion strategies into early phase clinical trials. Arginine auxotrophic tumors are reliant on extracellular arginine, due to the downregulation of arginos...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.30051
更新日期：2016-08-01 00:00:00
abstract：:A wide variety of primary and metastatic human neoplasms express somatostatin receptors (SS-Rs). We evaluated the SS-R status of malignant lymphomas that had been surgically removed from 31 patients by use of in vitro SS-R autoradiography with the SS analog 125I-[Tyr3]-octreotide as radioligand. Of 11 low-grade-malign...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910500613
更新日期：1992-04-01 00:00:00
abstract：:Fecal occult blood test (FOBT) screening for colorectal cancer (CRC) is implemented in several countries. Approximately half of all FOBT-positive persons have screen-detected adenomas. Despite removal of these, patients with large/multiple adenomas have increased risk of later developing new advanced adenomas and CRC....
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32850
更新日期：2020-08-15 00:00:00
abstract：:Claudin7 (cld7) is a cancer-initiating cell (CIC) marker in gastrointestinal tumors, a cld7-knockdown (kd) being accompanied by loss of tumor progression. Tumor exosomes (TEX) restoring CIC activities, we explored the contribution of cld7. This became particularly interesting, as tight junction (TJ)- and glycolipid-en...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32312
更新日期：2019-10-15 00:00:00
abstract：:The expression of the nuclear SV40-induced T antigen was measured by microfluorimetry on individual, asynchronously growing SV40-transformed cells which had been stained with hamster T-antiserum by the indirect immunofluorescence method. The same individual cells were first measured for T antigen and then for DNA by F...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910150403
更新日期：1975-04-15 00:00:00
abstract：:Children are especially sensitive to ionizing radiation and chemical carcinogens, and limiting their cancer risk is of great public concern. Calorie restriction (CR) is a potent intervention for suppressing cancer. However, CR is generally not appropriate for children. This study, therefore, examined to see if adult-o...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.28751
更新日期：2014-09-01 00:00:00
abstract：:In light of recent, strong inverse findings between lifetime red wine consumption and prostate cancer among younger men, we revisited our previous cohort analysis to more thoroughly investigate red wine consumption and prostate cancer in the Health Professionals Follow-up Study (HPFS). In 1986, HPFS participants repor...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22498
更新日期：2007-04-01 00:00:00
abstract：:The association between parity and hepatocellular carcinoma (HCC) was studied using a data-base generated by linking 2 Swedish nation-wide registries; the Cancer Registry and the Fertility Registry. Among women born between 1925 and 1960, 133 patients with HCC recorded in the Cancer Registry between 1958 and 1984 were...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910550508
更新日期：1993-11-11 00:00:00
abstract：:The large geographic variations in the incidence of gastric cancer (GC) are likely due to differential environmental exposures, in particular to Helicobacter pylori (H. pylori) infection. We aimed to investigate the impact of H. pylori on the epigenome in normal gastric mucosa and methylation changes associated with c...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.31381
更新日期：2018-08-01 00:00:00
abstract：:Chimeric bcr/abl fusion proteins are thought to be the molecular 'pathogen' of chronic myelogenous leukaemia (CML). Expression levels of the respective fusion RNAs reflect disease progression as well as remission upon therapeutic intervention in CML patients. However, there is no quick and reliable method that would a...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(20000601)86:5<741::aid-ijc
更新日期：2000-06-01 00:00:00
abstract：:To assess the excess risk of HPV-associated cancer (HPVaC) in two at-risk groups-women with a previous diagnosis of high grade cervical intraepithelial neoplasia (CIN3) and both men and women treated for non-cervical pre-invasive anogenital disease. All CIN3 cases diagnosed in 1989-2015 in Scotland were extracted from...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32126
更新日期：2019-07-15 00:00:00
abstract：:In this study we investigated the effect of Tax on nucleotide excision repair (NER) in human T-cell lines by using the host cell repair analysis of UVC-irradiated reporter plasmid. This analysis revealed a p53-dependent NER activity in wild type (w.t.) p53-containing T-cells and p53-independent NER in w.t. p53-lacking...
journal_title：International journal of cancer
pub_type： 杂志文章,收录出版
doi：10.1002/ijc.23091
更新日期：2008-01-15 00:00:00
abstract：:The androgen receptor (AR) gene is a transcription factor responsible for mediating the physiological effects of androgens. Evidence suggests that androgens and the androgen receptor are involved in uterine cell proliferation. A polymorphic CAG repeat in exon 1 of the AR gene encodes a polyglutamine tract that is inve...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.21436
更新日期：2006-03-01 00:00:00
abstract：:CD16 surface antigens represent activatory molecules in CD3-16+ NK cells. In order to target NK cells against relatively NK-resistant ovarian carcinomas, we used an anti-CD16 monoclonal antibody (MAb) (VD4), together with an anti-ovarian carcinoma-associated antigen (MOV19), to construct biMAbs. To this end, hybrid hy...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910480213
更新日期：1991-05-10 00:00:00
abstract：:Limited data are available on the carcinogenicity of smokeless tobacco products in organs other than the mouth. Snus is a smokeless tobacco product widely used in Norway. We studied 10,136 Norwegian men enrolled since 1966 in a prospective cohort study, 31.7% of whom were exposed to snus. The relative risk of pancreat...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.20811
更新日期：2005-05-10 00:00:00
abstract：:Meningioma, the most frequent tumor in the central nervous system, has few recognized risk factors. We explored the role of allergies in a population-based case-control consortium study of meningioma in five geographic areas. We also studied serum levels of a marker of atopic allergy (IgE) in a subset of study partici...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.25858
更新日期：2011-10-15 00:00:00
abstract：:Socioeconomic status (SES) is a determinant of outcome in various types of cancer. The aim of this study is to analyze the impact of the SES in Hodgkin's lymphoma (HL). From 2001 to 2005, 194 consecutive patients were prospectively followed in 5 institutions. Patients answered a questionnaire with a set of items used ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22417
更新日期：2007-02-15 00:00:00
abstract：:We have analysed expression of the first 7 members of the family of heparin-binding fibroblast growth factor (FGFs) and their 4 high-affinity receptors (FGFRs) in human pancreatic carcinoma cell lines, both at the mRNA and protein levels. In cell lines expressing FGFRs, 2 typical patterns were observed: (i) expression...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910590515
更新日期：1994-12-01 00:00:00
abstract：:The association between certain human papillomaviruses (HPV) and cervical intraepithelial neoplasia (CIN) is well documented, but there is uncertainty about the strength of association and the role of co-factors is unclear. This population-based case-control study in Norwegian women 20-44 years of age included 103 cas...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910610306
更新日期：1995-05-04 00:00:00
abstract：:To assess the effect of different hospital types or surgical volume on the survival of ovarian cancer patients, a nationwide and population-based analysis was carried out in Finland. The study included all 3,851 ovarian cancer patients operated from 1983-94. The patients were classified according to the hospital of th...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.10754
更新日期：2002-12-10 00:00:00
abstract：:Telomerase activity is involved in the maintenance of telomere length and is thought to be required for cellular immortality and oncogenesis. Three major subunits composing telomerase, human telomerase RNA (hTR), telomerase-associated protein (TPI) and human telomerase catalytic subunit (hTERT), have been identified. ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19990315)80:6<804::aid-ijc
更新日期：1999-03-15 00:00:00
abstract：:Murine macrophages from different anatomical sites were compared for their ability to become tumoricidal and to secrete interleukin-1 (IL-1) and tumor necrosis factor (TNF) following stimulation in vitro by several biological response modifiers (BRM). Peritoneal macrophages (PM), alveolar macrophages (AM), and tumor-i...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910480513
更新日期：1991-07-09 00:00:00
abstract：:Replacement of normal urothelium by pre-cancerous epithelium may explain the high recurrence rate of human bladder cancer. An in vitro model was designed in order to study the mechanisms of expansion of transformed urothelial cells at the expense of normal urothelium. For this purpose, mouse bladder explants were allo...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910540520
更新日期：1993-07-09 00:00:00
abstract：:The APTIMA HPV Assay (AHPV) allows detection of 14 high-risk human papillomavirus (HPV) RNA types in cervical specimens. Until present, the assay has been compared to HPV DNA tests only in triage settings. Herein, we compare AHPV with a DNA assay (Hybrid Capture 2; HC2) and liquid-based cytology (LBC; using PreservCyt...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.25726
更新日期：2011-08-01 00:00:00
abstract：:Commercial cow milk contains considerable amounts of estrogens. Our study assessed the effect of commercial low-fat milk on the development of 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary tumors in rats. Eighty 6-week-old female Sprague-Dawley rats received a single oral dose of 5 mg DMBA. Twenty-four hours l...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.20172
更新日期：2004-07-01 00:00:00
