Abstract:
:The size of the genome in the opportunistic fungus Candida albicans is 15.6 Mb. Whole-genome shotgun sequencing was carried out at Stanford University where the sequences were assembled into 412 contigs. C. albicans is a diploid basically, and analysis of the sequence is complicated due to repeated sequences and to sequence polymorphism between homologous chromosomes. Chromosome 7 is 1 Mb in size and the best characterized of the 8 chromosomes in C. albicans. We assigned 16 of the contigs, ranging in length from 7309 to 267,590 bp, to chromosome 7 and determined sequences of 16 regions. These regions included four gaps, a misassembled sequence, and two major repeat sequences (MRS) of >16 kb. The length of the continuous sequence attained was 949,626 bp and provided complete coverage of chromosome 7 except for telomeric regions. Sequence analysis was carried out and predicted 404 genes, 11 of which included at least one intron. A 7-kb indel, which might be caused by a retrotransposon, was identified as the largest difference between the homologous chromosomes. Synteny analysis revealed that the degree of synteny between C. albicans and Saccharomyces cerevisiae is too weak to use for completion of the genomic sequence in C. albicans.
journal_name
Geneticsjournal_title
Geneticsauthors
Chibana H,Oka N,Nakayama H,Aoyama T,Magee BB,Magee PT,Mikami Ydoi
10.1534/genetics.104.034652keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
1525-37issue
4eissn
0016-6731issn
1943-2631pii
genetics.104.034652journal_volume
170pub_type
杂志文章相关文献
GENETICS文献大全abstract::l(3)S12 is a vital locus whose function is required in embryos, early larvae, late pupae and oogenesis. We have identified a cold-sensitive allele, l(3)S12(3), and characterized conditional misexpression of the gene associated with this mutation as well as with several euchromatic insertions of l(3)S12+ transposons. S...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::Transposable elements are often considered as selfish DNA sequences able to invade the genome of their host species. Their evolutive dynamics are complex, due to the interaction between their intrinsic amplification capacity, selection at the host level, transposition regulation, and genetic drift. Here, we propose mo...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.031211
更新日期:2005-02-01 00:00:00
abstract::Drosophila melanogaster males deficient for the crystal (cry) locus of the Y chromosome that carry between 15 and 60 copies of the X-linked Stellate (Ste) gene are semisterile, have elevated levels of nondisjunction, produce distorted sperm genotype ratios (meiotic drive), and evince hyperactive transcription of Ste i...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-08-01 00:00:00
abstract::Gene silencing by heterochromatin is a well-known phenomenon that, in Drosophila, is called position effect variegation (PEV). The long-held hypothesis that this gene silencing is associated with an altered chromatin structure received direct support only recently. Another gene-silencing phenomenon in Drosophila, alth...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::An analysis of the effects of spontaneous mutations affecting age-specific mortality was conducted using 29 lines of Drosophila melanogaster that had accumulated spontaneous mutations for 19 generations. Divergence among the lines was used to estimate the mutational variance for weekly mortality rates and the covarian...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::R1 and R2 elements are non-LTR retrotransposons that insert specifically into the 28S rRNA genes of arthropods. The process of concerted evolution of the rDNA locus should give rise to rapid turnover of these mobile elements compared to elements that insert at sites throughout a genome. To estimate the rate of R1 and ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract::We consider resequencing studies of associated loci and the problem of prioritizing sequence variants for functional follow-up. Working within the multivariate linear regression framework helps us to account for the joint effects of multiple genes; and adopting a Bayesian approach leads to posterior probabilities that...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.184572
更新日期:2016-02-01 00:00:00
abstract::Cryptococcus neoformans is a fungal pathogen that causes meningitis in immunocompromised hosts. The organism has a known sexual cycle, and strains of the MATalpha mating type are more virulent than isogenic MATa strains in mice, and they are more common in the environment and infected hosts. A C. neoformans homolog of...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Grain yield is a major goal for the improvement of durum wheat, particularly in drought-prone areas. In this study, the genetic basis of grain yield (GY), heading date (HD), and plant height (PH) was investigated in a durum wheat population of 249 recombinant inbred lines evaluated in 16 environments (10 rainfed and 6...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.077297
更新日期:2008-01-01 00:00:00
abstract::As part of our investigation of disease resistance in lettuce, we generated mutants that have lost resistance to Bremia lactucae, the casual fungus of downy mildew. Using a rapid and reliable screen, we identified 16 distinct mutants of Latuca sativa that have lost activity of one of four different downy mildew resist...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract::Accumulation of RNA was measured in adult males of two genotypes: car bb/Ybb- and car bb/YbbSuVar-5. The two genotypes have similar amounts of rDNA, which is reduced in comparison to wild type (CLARK, STRAUSBAUGH and KIEFER 1977). Although genotypically bobbed, car bb/YbbSuVar-5 flies have a wild-type phenotype; car b...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-11-01 00:00:00
abstract::Many of the transcription factors and target genes that pattern the developing adult remain unknown. In the present study, we find that an ortholog of the poorly understood transcription factor, glucose transporter (GLUT4) enhancer factor (Glut4EF, GEF) [also known as the Huntington's disease gene regulatory region-bi...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.078030
更新日期:2008-02-01 00:00:00
abstract::Previously we have shown that the nucleolus organizer region (NOR) of Neurospora crassa changes size frequently during the premeiotic portion of the sexual phase. Here, we have investigated whether these changes in size originate only in specific regions of the NOR, or are distributed throughout the NOR. In two specia...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::Phosphatidylinositol catabolism in Saccharomyces cerevisiae cells cultured in media containing inositol results in the release of glycerophosphoinositol (GroPIns) into the medium. As the extracellular concentration of inositol decreases with growth, the released GroPIns is transported back into the cell. Exploiting th...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Two-dimensional paper chromatography was performed on methanol extracts of leaves of hexaploid bread wheat, Triticum aestivum L. em. Thell. cultivar Chinese Spring, and of the available nullisomic-tetrasomic compensating lines, the tetrasomic lines and the ditelocentric lines. The chromatograms had 27 spots identified...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1983-02-01 00:00:00
abstract::Genomic sequences provide powerful new tools in genetic analysis, making it possible to combine classical genetics with genomics to characterize the genes in a particular chromosome region. These approaches have been applied successfully to the euchromatin, but analysis of the heterochromatin has lagged somewhat behin...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.2.807
更新日期:2004-02-01 00:00:00
abstract::Obligate pathogenic and endosymbiotic bacteria typically experience gene loss due to functional redundancy, asexuality, and genetic drift. We hypothesize that reduced genomes increase their functional complexity through protein multitasking, in which many genes adopt new roles to counteract gene loss. Comparisons of i...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.145656
更新日期:2013-01-01 00:00:00
abstract::The small ovary gene (sov) is required for the development of the Drosophila ovary. Six EMS-induced recessive alleles have been identified. Hypomorphic alleles are female sterile and have no effect on male fertility, whereas more severe mutations result in lethality. The female-sterile alleles produce a range of mutan...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
abstract::The pathogenic life cycle of the rice blast fungus Magnaporthe oryzae involves a series of morphogenetic changes, essential for its ability to cause disease. The smo mutation was identified > 25 years ago, and affects the shape and development of diverse cell types in M. oryzae, including conidia, appressoria, and asc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301490
更新日期:2019-01-01 00:00:00
abstract::To understand the role of Los1p in pre-tRNA splicing, we sought los1 multicopy suppressors. We found SOL1 that suppresses both point and null LOS1 mutations. Since, when fused to the Ga14p DNA-binding domain, Los1p activates transcription, we tested whether Los1p regulates SOL1. We found that las1 mutants have deplete...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::Understanding variation in allele frequencies across populations is a central goal of population genetics. Classical models for the distribution of allele frequencies, using forward simulation, coalescent theory, or the diffusion approximation, have been applied extensively for demographic inference, medical study des...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.200493
更新日期:2017-07-01 00:00:00
abstract::Clinal variation is common for enzymes in the glycolytic pathway for Drosophila melanogaster and is generally accepted as an adaptive response to different climates. Although the enzyme phosphoglucomutase (PGM) possesses several allozyme polymorphisms, it is unique in that it had been reported to show no clinal variat...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::Three sex-transforming genes (ix, tra-D, and dsx) in D. melanogaster were examined with respect to possible interactions with the NSR strain SR-spirochetes. The SR-spirochetes exerted a lethal effect on XY but not on XX individuals, regardless of whether they were phenotypically males, intersexes, or females. These re...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::The contributions of direct and inverted repeats to deletion formation were studied by characterizing Ampr revertants of plasmids with a series of insertion mutations at a specific site in the pBR322 ampicillin resistance (amp) gene. The inserts at this site are palindromic, variable in length, and bracketed by 9- or ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
abstract::We propose a new method for calculating probabilities for pedigree genetic data that incorporates crossover interference using the chi-square models. Applications include relationship inference, genetic map construction, and linkage analysis. The method is based on importance sampling of unobserved inheritance pattern...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-08-01 00:00:00
abstract::Crossovers (COs) generated through meiotic recombination are important for the correct segregation of homologous chromosomes during meiosis. Several models describing the molecular mechanism of meiotic recombination have been proposed. These models differ in the arrangement of heteroduplex DNA (hDNA) in recombination ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.070367
更新日期:2007-05-01 00:00:00
abstract::Sequences of Bronze2 (Bz2), a maize gene which is required for the synthesis of the purple pigment anthocyanin, have been cloned by combining the techniques of transposon tagging and differential hybridization. First, a mutable bz2 allele (bz2-mu1) was recovered from a Mutator line. The mutation was assumed to result ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::The nuclear envelope in Saccharomyces cerevisiae harbors two essential macromolecular protein assemblies: the nuclear pore complexes (NPCs) that enable nucleocytoplasmic transport, and the spindle pole bodies (SPBs) that mediate chromosome segregation. Previously, based on metazoan and budding yeast studies, we report...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.141465
更新日期:2012-10-01 00:00:00
abstract::We examine the efficiency of different genotyping and phenotyping strategies in inbred line crosses from an information perspective. This provides a mathematical framework for the statistical aspects of QTL experimental design, while guiding our intuition. Our central result is a simple formula that quantifies the fra...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.038612
更新日期:2005-05-01 00:00:00
abstract::The Collaborative Cross (CC) was designed to facilitate rapid gene mapping and consists of hundreds of recombinant inbred lines descended from eight diverse inbred founder strains. A decade in production, it can now be applied to mapping projects. Here, we provide a proof of principle for rapid identification of major...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.163014
更新日期:2014-09-01 00:00:00