Abstract:
:Calcium-independent nitric oxide synthase (NOS) activity has been reported in ischemic brains and usually attributed to the inducible isoform, iNOS. Because calcium-independent mechanisms have recently been shown to regulate the constitutive calcium-dependent NOS, we proposed to confirm the presence of iNOS activity in our model of transient focal cerebral ischemia in rats. Our initial results showed that, in our model, ischemia induced an important increase in brain calcium concentration. Consequently, the determination of calcium-independent NOS activity required a higher concentration of calcium chelator than classically used in the NOS assay. In these conditions, calcium-independent NOS activity was not observed after ischemia. Moreover, our ischemia was associated with neither iNOS protein expression, measured by Western blotting, nor increased NO production, evaluated by its metabolites (nitrate/nitrite). Our results demonstrate that iNOS activity may be overestimated due to increased brain calcium concentration in ischemic conditions and also that iNOS is not systematically induced after cerebral ischemia.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Lerouet D,Jafarian-Tehrani M,Louin G,Palmier B,Bonnefont-Rousselot D,Plotkine M,Margaill Idoi
10.1016/j.expneurol.2005.05.001keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
218-28issue
1eissn
0014-4886issn
1090-2430pii
S0014-4886(05)00170-6journal_volume
195pub_type
杂志文章abstract::Many neurological and psychiatric disorders exhibit gender disparities, and sex differences in the brain likely explain some of these effects. Recent work in rodents points to a role for epigenetics in the development or maintenance of neural sex differences, although genome-wide studies have so far been lacking. Here...
journal_title:Experimental neurology
pub_type: 杂志文章,评审
doi:10.1016/j.expneurol.2014.08.006
更新日期:2015-06-01 00:00:00
abstract::The effects of superior cervical ganglion stimulation and the local application of norepinephrine or its antagonist propranolol were studied in 18 pineal cells. In 61% of the pineal cells, stimulation elicited excitation; the same cells also responded by excitation to norepinephrine ejection. When stimulation and nore...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90149-9
更新日期:1985-12-01 00:00:00
abstract::Previously injured dorsal roots were electrically stimulated to determine if regenerating sensory axons can form physiologically active synaptic contacts with neurons within fetal spinal cord tissue transplants. Dorsal rootlets, sectioned at their spinal cord entry zone, were apposed to intraspinal transplants of feta...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1996.0101
更新日期:1996-06-01 00:00:00
abstract::Parkinson's disease (PD) is associated with complex compensatory changes in the functional and neurochemical anatomy of the basal ganglia resulting from striatal dopamine deficiency. Available evidence suggests that glutamatergic corticostriatal pathway becomes overactive following nigrostriatal dopaminergic denervati...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2003.12.012
更新日期:2004-05-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a progressive and fatal neurodegenerative disease characterized by motor neuron death. Since most ALS patients succumb to ventilatory failure from loss of respiratory motor neurons, any effective ALS treatment must preserve and/or restore breathing capacity. In rats over-expressi...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2015.08.011
更新日期:2015-11-01 00:00:00
abstract::One approach to repair of transected nerves is to attempt extrinsic guidance of axons across the gaps. We inserted the proximal and distal stumps of severed mouse sciatic nerves into opposite ends of biodegradable polyester tubes. The nerves and ensheathing tubes were examined after postoperative survival times of as ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90162-1
更新日期:1985-12-01 00:00:00
abstract::Stress and glucocorticoids exacerbate pain via undefined mechanisms. Macrophage migration inhibitory factor (MIF) is a constitutively expressed protein that is secreted to maintain immune function when glucocorticoids are elevated by trauma or stress. Here we show that MIF is essential for the development of neuropath...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2012.04.018
更新日期:2012-08-01 00:00:00
abstract::Neuronal activity in the locus ceruleus of awake, behaving stump-tailed monkeys increased consistently and vigorously after presentation of mildly aversive and threatening stimuli. These preliminary data increase the range of stimuli reported to elicit locus ceruleus activity in awake monkeys to include nonpainful thr...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(84)90217-6
更新日期:1984-06-01 00:00:00
abstract::Axonal lesions to the optic nerve (ON) induce c-Jun expression in retinal ganglion cells (RGCs) of the rat in vivo. Detailed investigations using retrograde tracers, and double labeling studies for c-Jun and regeneration-associated factors, such as the growth-associated protein GAP-43, have suggested that this upregul...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1997.6585
更新日期:1997-09-01 00:00:00
abstract::Muscular dystrophies (MDs) consist of a genetically heterogeneous group of disorders, recessive or dominant, characterized by progressive skeletal muscle weakening. To date, no effective treatment is available. Experimental strategies pursuing muscle regeneration through the transplantation of stem cell preparations h...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2009.08.002
更新日期:2009-11-01 00:00:00
abstract::Rat axial muscle previously has not been studied histochemically. We were interested in determining the fiber composition and fiber distribution in rat lateral longissimus (LL), the large epaxial dorsiflexor muscle active during sexual posturing in the female rat and to determine if estrogen replacement in ovariectomi...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(83)90229-7
更新日期:1983-02-01 00:00:00
abstract::The possible pain inhibitory effects of periaqueductal gray (PAG) stimulation were investigated in cats anesthetized with Nembutal and immobilized with Flaxedil. Unitary responses evoked by electrical stimulation of the upper canine dentine and by cutaneous facial noxious and nonnoxious stimuli were recorded extracell...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(83)90155-3
更新日期:1983-07-01 00:00:00
abstract::Ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) have been shown both in vitro and in vivo to support the survival and differentiation of developing chick or rat motoneurons. To explore the potential use of these and other neurotrophic factors as therapeutic agents in human motoneuron di...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1994.1198
更新日期:1994-12-01 00:00:00
abstract::Infants born very preterm are at high risk for neurological deficits including cerebral palsy. In this study we assessed the neuroprotective effects of umbilical cord blood cells (UCBCs) and optimal administration timing in a fetal sheep model of preterm brain injury. 50 million allogeneic UCBCs were intravenously adm...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2016.06.017
更新日期:2016-09-01 00:00:00
abstract::A graded contusion spinal cord injury (SCI) was created in the adult rat spinal cord using the Infinite Horizons (IH) impactor to study the correlation between injury severity and anatomical, behavioral, and electrophysiological outcomes. Adult Fisher rats were equally divided into five groups and received contusion i...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2004.08.026
更新日期:2005-02-01 00:00:00
abstract::A "neuroplastic" hypothesis proposes that changes in neuronal structural plasticity may underlie the aetiology of depression and the action of antidepressants. The medial prefrontal cortex (mPFC) is affected by this disorder and shows an intense expression of the polysialylated form of the neural cell adhesion molecul...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2008.07.018
更新日期:2008-11-01 00:00:00
abstract::Cervical injuries are the most common form of SCI. In this study, we used a neuromodulatory approach to promote skilled movement recovery and repair of the corticospinal tract (CST) after a moderately severe C4 midline contusion in adult rats. We used bilateral epidural intermittent theta burst (iTBS) electrical stimu...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2017.08.004
更新日期:2017-11-01 00:00:00
abstract::The beta-Amyloid peptide (A beta) is hypothesized to mediate the neurodegeneration seen in Alzheimer's disease. Recently, we proposed a new hypothesis to explain the toxicity of A beta based on the free-radical generating capacity of A beta. We have recently demonstrated using electron paramagnetic resonance (EPR) spe...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(95)90041-1
更新日期:1995-02-01 00:00:00
abstract::Injection of lysosomal enzyme into cisternal or ventricular cerebrospinal fluid (CSF) has been carried out in 11 lysosomal storage disorder models, with each study demonstrating reductions in primary substrate and secondary neuropathological changes, and several reports of improved neurological function. Whilst acute ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2014.09.008
更新日期:2015-01-01 00:00:00
abstract::Hemiparkinson-hemiatrophy syndrome (HP-HA) is associated with skeletal hemiatrophy and the later development of parkinsonism. It is generally assumed that this phenotype is due to the combination of dysfunction of the basal ganglia (e.g., substantia nigra compacta and/or other related structures), causing parkinsonism...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2002.8005
更新日期:2002-10-01 00:00:00
abstract::Systemic administration of kainic acid (12 mg/kg, i.p.), causing the established epileptic syndrome and the associated specific pattern of limbic nerve cell loss, led to substantial time-dependent increases in the brain metabolism of the endogenous excitotoxin, quinolinic acid. These changes preferentially affected bi...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(88)90140-9
更新日期:1988-01-01 00:00:00
abstract::In seven families from a Siberian founder population with autosomal dominant spinocerebellar ataxia (SCA) genetic analysis of the polymorphisms flanking the SCA1 locus on chromosome 6p showed allelic association with disease inheritance. While the association was absolute in the case of microsatellite D6S274, an allel...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1994.1070
更新日期:1994-04-01 00:00:00
abstract::After optic nerve transection beta cells of cat retinal ganglion cells (RGCs) suffer from rapid cell death from 3 to 7 days, whereas alpha cells gradual cell death until 14 days. Here we report electrophysiological properties of Y- (morphological alpha) and X- (morphological beta) cells at 5 and 14 days after axotomy ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2002.7958
更新日期:2002-09-01 00:00:00
abstract::The effects of methylmercury (MeHg) on cytoplasmic microtubules in cultured neuroblastoma cells, glioma cells, and fibroblasts were compared. Neuroblastoma cells appeared to be more sensitive to disruption of microtubules by MeHg than the glioma cells or fibroblasts; cellular concentrations of mercury after MeHg were ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(84)90147-x
更新日期:1984-08-01 00:00:00
abstract::Brain-derived neurotrophic factor (BDNF) plays an essential role in sensory neuronal activation in response to visceral inflammation. Here we report that BDNF up-regulation in the primary afferent neurons in the dorsal root ganglia (DRG) in a rat model of colitis is mediated by the activation of endogenous extracellul...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2012.08.007
更新日期:2012-12-01 00:00:00
abstract::The recently discovered endocannabinoid system (ECS), which includes endocannabinoids and the proteins that metabolize and bind them, has been implicated in multiple regulatory functions both in health and disease. Several studies have suggested that ECS is centrally and peripherally involved in the processing of pain...
journal_title:Experimental neurology
pub_type: 杂志文章,评审
doi:10.1016/j.expneurol.2010.03.029
更新日期:2010-07-01 00:00:00
abstract::The therapeutic effect of glial progenitor transplantation in diseases of dysmyelination is currently attributed to the formation of new myelin. Using magnetic resonance imaging (MRI), we show that the therapeutic outcome in dysmyelinated shiverer mice is dependent on the extent of cell migration but not the presence ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2017.02.005
更新日期:2017-05-01 00:00:00
abstract::With the use of four independent cell markers and Brd-U birthdating we have charted the earliest stages of neuronal differentiation and migration in the developing rat central nervous system, including the cortex, spinal cord, and retina. One of the markers, the monoclonal antibody 2G12, labeled a large subpopulation ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1995.1031
更新日期:1995-07-01 00:00:00
abstract::Huntington's disease (HD) is a hereditary, progressive and ultimately fatal neurodegenerative disorder. Excitotoxicity and reduced availability of neurotrophic factors (NTFs) likely play roles in HD pathogenesis. Recently we developed a protocol that induces adult human bone marrow derived mesenchymal stem cells (MSCs...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2011.12.045
更新日期:2012-04-01 00:00:00
abstract::Axons of retinal ganglion cells (RGCs) of adult cats were transected and regenerated by the transplantation of peripheral nerves. Two months after transplantation dendritic arbors of RGCs with regenerated axons (r-RGCs) were visualized with intracellular injections of horseradish peroxidase (HRP). The HRP-filled r-RGC...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1996.6345
更新日期:1997-01-01 00:00:00