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Reverse transcriptase-PCR for the detection of Astrovirus in children with nosocomial acute diarrhoea in Naples, Italy.

Abstract：

OBJECTIVE:This study was made to determine the incidence of nosocomial viral gastroenteritis in all children aged 0-4 years, admitted in the Pediatric Hospital over a 3-year period. METHODS:Astrovirus was detected by reverse transcriptase-PCR; routine diagnostic tests for Rotavirus, Adenovirus, and common bacterial pathogens were carried out on all samples. RESULTS:Of the 460 children with nosocomial diarrhea, 23 harbored Astrovirus (5%). Most cases occurred during the coldest months of the year. Children under 1 year of age were the most susceptible population. CONCLUSION:The collected data confirms the importance of viral etiology in nosocomial gastroenteritis. The reported rate of detection stresses the importance of Astrovirus in pediatric diarrhea. The authors recommend screening for this virus on a routine basis.

journal_name

Med Mal Infect

journal_title

Medecine et maladies infectieuses

authors

Galdiero E,Marinelli A,Pisciotta MG,Pagliara I,Di Monteforte ES,Liguori G

doi

10.1016/j.medmal.2005.03.006

keywords:

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

213-7

issue

4

eissn

0399-077X

issn

1769-6690

pii

S0399-077X(05)00066-1

journal_volume

35

pub_type

杂志文章

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