Abstract:
:Transplantation of hands in humans has become an accepted therapeutic modality. A hand transplant is composed of various tissues of different degree of immunogenicity. In addition, skin contains own resident bacterial flora that may become virulent in the ischemic and rejecting graft. Discrimination between skin rejection and bacterial inflammation is difficult. The aim of our experimental study was to investigate the cellular reaction to skin bacteria and alloantigen in the regional lymph nodes draining skin and analyse the changes in cell phenotypes. The study was carried out on rats inoculated into hind-limb paw either with S. epidermidis or allogeneic peripheral blood mononuclear cells. An increase in lymph node weight and cell concentration was observed after bacterial infection. After stimulation with allogeneic cells, node mass increased significantly but its cell number rose much less. The percentage of lymph node W3/13 (leukocytes, T cells), W3/25 (CD4), OX8 (CD8), OX6 (class II), OX12 (B cells), EDI (CD14), CD31 (lymphocytes), OX7 (stem cells), and OX62 (migrating dendritic) cells did not change in both groups compared to controls. There was a significant rise of the CD54 (ICAM I) subset after bacterial infection and increase in percentage of OX8-cytotoxic and decrease of W3/25 (helper) subset after allogeneic stimulation. Lack of major differences between stimulated and control contralateral nodes may be explained by systemic reaction to the tested antigens affecting also nodes on the non-injected side. Comparison of the reaction of bacteria and alloantigen stimulated nodes revealed an increase in percentage of OX6, OX12, CD31, CD54, OX33 and OX62 after infection with S. epidermidis, whereas allostimulation brought about only rise in T (W3/13) cell population. Neither stimulation caused increase in expression of class II antigens on T cells. The obtained results demonstrate evident differences in type of response to bacteria and alloantigens. To what extent can bacterial stimulation enhance allogeneic reaction is now under study. Our data are helpful in understanding differences in the character and kinetics of reaction to both types of antigens and may taken into consideration when planning therapy in recipients of hand allografts, immunosuppressive drugs or antibiotics.
journal_name
Ann Transplantjournal_title
Annals of transplantationauthors
Cakala M,Olszewski WLkeywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
59-62issue
4eissn
1425-9524issn
2329-0358journal_volume
9pub_type
杂志文章abstract:BACKGROUND:Graft-versus-host disease (GvHD) is the most important complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT) responsible for increased mortality. Recent studies have discussed the use of oral GvHD screening tests in the diagnosis of systemic GvHD. This is the first study of the si...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/aot.883462
更新日期:2012-07-01 00:00:00
abstract::BACKGROUND Inflammatory activity of the artery can be assessed by measuring 18F-fluorodeoxyglucose (18F-FDG) uptake with positron emission tomography computed tomography (PET/CT). Improvement in vascular function after renal transplantation has been reported, but no studies have used 18F-FDG PET/CT to examine the chan...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/AOT.909212
更新日期:2018-06-15 00:00:00
abstract::BACKGROUND Chronic allograft nephropathy (CAN) remains a major problem for long-term graft survival and different pathways participate in its development. CXC chemokine receptor 4 (CXCR4) is significantly upregulated following renal injury and fibrotic response. We investigated the effect of AMD3100, a CXCR4 antagonis...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/AOT.899492
更新日期:2016-11-25 00:00:00
abstract:BACKGROUND:Despite documented positive effect of MachinePerfusion (MP) on long-term kidneys-graft function its wide use is restricted due to higher costs. The aim of this study was to analyze the difference in costs of kidney transplantation in patients who received organ stored in ColdStorage (CS) vs those who receive...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2009-04-01 00:00:00
abstract::Recovery of the patients after transplantation of non-renal organs may be complicated by multi-faceted chronic renal failure (CKD) which is regarded as an independent risk factor of graft dysfunction and mortality. The occurrence of CKD in non-renal transplant recipients depends mainly on a type of transplanted organ,...
journal_title:Annals of transplantation
pub_type: 杂志文章,评审
doi:
更新日期:2005-01-01 00:00:00
abstract::BACKGROUND The prognosis of the patients of acute liver failure (ALF) with onset of hepatic coma is often dismal. ALF is a well-accepted indication for liver transplantation (LT) and has markedly improved the prognosis of these patients. However, its role in ALF patients with onset of hepatic coma has never been eluci...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2018-03-13 00:00:00
abstract:BACKGROUND:Significant geographic disparities exist in access to liver transplantation and consequently the current liver allocation system is being challenged. We sought to describe our unique experience with using organs with long cold ischemia times from the largest donation service area. MATERIAL AND METHODS:From ...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/aot.896190
更新日期:2016-03-08 00:00:00
abstract::Transplantation medicine, like no other medical specialty, is connected with a wide range of very difficult ethical issues. Among those are: availability of the treatment, consent for cadaveric donation, allocation of available organs, new categories of living unrelated donors and economic aspects of this method of tr...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::A short review of legal aspects of organ transplantation. The influence of type of consent and the family attitude on organ donation rate in all European countries. A special attention is given to the legal and ethical aspects of living organ and tissue transplantation. ...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Infection remains the most common complication following renal transplantation (RT). Cytomegalovirus (CMV) is one of the most important pathogens in this patient population and Clostridium difficile-associated colitis (CDAC) has emerged as one of the most frequent gastrointestinal complications. Only few cas...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2010-10-01 00:00:00
abstract::BACKGROUND Organ transplantation is one of the most critical topics in medical ethics that is commonplace in various countries. This study aimed to evaluate the knowledge and the ethical issues surrounding organ transplantation and organ donation among healthcare personnel in Tehran, Iran. MATERIAL AND METHODS In a cr...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/AOT.908615
更新日期:2018-05-04 00:00:00
abstract:OBJECTIVE:Hormonal alterations caused by chronic liver disease are well described. In contrast, the reversibility of peripheral hormonal changes after orthotopic liver transplantation (OLT) has only partially been analyzed since the establishment of OLT as treatment of chronic hepatic failure. In addition it is not fin...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:Data in kidney transplant recipients regarding elimination of calcineurin inhibitor (CNI) therapy from a de novo regimen based on low CNI exposure and an mTOR inhibitor are sparse, and restricted to CNI elimination within the first six months post-transplant. MATERIAL/METHODS:In a 12-month, randomized, mult...
journal_title:Annals of transplantation
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.12659/aot.882637
更新日期:2012-01-01 00:00:00
abstract::BACKGROUND Long-term real-world data are relatively sparse regarding recurrence of chronic hepatitis B virus (HBV) infection after liver transplantation using hepatitis B immunoglobulin (HBIg) and nucleos(t)ide analogue (NUC) prophylaxis. MATERIAL AND METHODS Data from 371 adults transplanted for HBV-related disease a...
journal_title:Annals of transplantation
pub_type: 杂志文章,多中心研究
doi:10.12659/AOT.910176
更新日期:2018-11-13 00:00:00
abstract::BACKGROUND In kidney transplantation, the association of cold ischemia time (CIT), anastomosis time (AT), and delayed graft function (DGF) is particularly detrimental in grafts from marginal donors; however, actual cut-off criteria are still debated. MATERIAL AND METHODS Data from patients >65 years (n=193) and patien...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/aot.896672
更新日期:2016-05-31 00:00:00
abstract:BACKGROUND:Studies have documented deficits of pneumococcal and other vaccinations in kidney and lung transplant patients, but the vaccination status of liver transplant (LT) recipients is unknown. This cross-sectional study investigated the prevalence of immunizations among LT patients at a large university medical ce...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/aot.896436
更新日期:2016-04-07 00:00:00
abstract:BACKGROUND:This retrospective single-center study was undertaken to assess the occurrence of de novo neoplasms in renal transplant recipients according to the immunosuppressive regimen and time after transplantation. MATERIAL/METHODS:Observation encompassed 1028 patients transplanted between the years 1983-2006 and fo...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2011-04-01 00:00:00
abstract::BACKGROUND Laparoscopic donor nephrectomy (LDN) has evolved and has been established as a surgical standard of care for kidney transplantation. MATERIAL AND METHODS This study retrospectively reviews 1132 patients who underwent 4 different laparoscopic living-donor nephrectomies: hand-assisted laparoscopic nephrectomy...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/AOT.918189
更新日期:2020-02-11 00:00:00
abstract::BACKGROUND The purpose of this study was to assess the effect of an organ transplantation scandal on the rate of corneal donations and organ donors at the University Hospital Tübingen. MATERIAL AND METHODS Data from all hospital deaths from January 2012 to December 2013 were analyzed retrospectively. Each deceased pat...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/aot.901385
更新日期:2017-07-11 00:00:00
abstract:BACKGROUND:Breathing is essential for living. When someone is a lung transplantation candidate, it could be imagined that the breathing difficulties that he/she is experiencing has a great impact on his/her health status. MATERIAL/METHODS:We asked all the patients on the lung transplantation waiting list at Masih Dane...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::BACKGROUND Prognostic models for 3-year mortality after kidney transplantation based on pre-transplant donor and recipient variables may avoid futility and thus improve donor organ allocation. MATERIAL AND METHODS There were 1546 consecutive deceased-donor kidney transplants in adults (January 1, 2000 to December 31, ...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/AOT.913217
更新日期:2019-05-17 00:00:00
abstract:BACKGROUND:The number of transplant surgeries across the globe has markedly increased in recent decades and is associated with improvement in outcomes. Factors that could improve the organization of post-transplant care are sought. The aim of our study was to evaluate the changes in post-transplant care in the largest ...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/aot.895981
更新日期:2016-04-05 00:00:00
abstract:BACKGROUND:Xenotransplantation from pig to humans may be associated with the risk of transmission of porcine endogenous retroviruses (PERVs) that are present in the genome of all pigs and that infect human cells in vitro. However, it remains unclear whether PERVs infect transplant recipients in vivo and, if so, whether...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Markers currently used to detect kidney damage are effective in both early (KIM-1, NGAL) and late (MCP-1, MMP, TIMP) stages of renal tubular damage, indicating the progression of chronic kidney disease. Immunosuppressive drugs may damage the transplanted organ through their direct toxic effects and by contri...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/aot.895275
更新日期:2016-03-03 00:00:00
abstract::Irrespectively of universal shortage of donor organs there is a tendency of increasing the number of transplantations from living and deceased donors. Each of these two methods has positive and negative features. The main obstacles using living donors are health hazard, necessity to solve certain donor's social and ps...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:The objective was to evaluate contrast enhanced ultrasound (CEUS) based cholangiography compared to conventional radiography as a reference method in patients after liver transplantation. MATERIAL/METHODS:Contrast agents were administered through T-tubes, which were placed during the operation. Twelve patie...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/aot.883700
更新日期:2012-12-31 00:00:00
abstract:BACKGROUND:Expansion of the donor pool by the use of grafts with extended donor criteria reduces waiting list mortality with an increased risk for graft and patient survival after liver transplantation. This study investigates the ability of the Donor-Risk-Index (DRI), the Extended-Criteria-Donor-Score (ECD-score) and ...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/aot.883452
更新日期:2012-07-01 00:00:00
abstract::BACKGROUND Acute graft-versus-host disease (aGVHD) remains the most common and challenging complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). An important obstacle to the therapeutic effect of aGVHD is the inability to identify risk factors for an individual patient at the onset of symp...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:10.12659/aot.901495
更新日期:2017-02-03 00:00:00
abstract:OBJECTIVE:Extensive stricture of ureter still poses a difficult challenge for urologic surgons, especially when its middle part is affected. We present a reconstructive method of long ureteral strictures with use of xenogenic collagen membranes as a scaffolding to promote new tissue growth in the area of extensive uret...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Anti-CMV prophylaxis is currently a routine management in patients after organ transplantation. One of the clinical symptoms of CMV infection may be lesions in the oral cavity.The aim of this study was to determine the relationship between CMV infection, occurrence of Candida species and presence of oral muc...
journal_title:Annals of transplantation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00