Abstract:
:Enterocin CRL35 activity is deeply influenced by the membrane viscosity as could be demonstrated performing determinations of the minimal inhibitory concentrations (MIC) at different temperatures and analyzing the membrane viscosity in these cells as well as in resistant bacteria. In all the cases, bacteriocin activity was linked to higher levels of viscosity. This finding was confirmed studying the interaction of enterocin CRL35 with liposomes composed of dimyristoyl phosphatidylcholine: dimyristoyl phosphatidylglycerol (9:1) in both gel and liquid-crystalline phases. It could be establish, from peptide insertion analysis following the tryptophan fluorescence and microviscosity experiments that this peptide is able to interact more efficiently with membranes having a more structured hydrophobic core.
journal_name
Biochimiejournal_title
Biochimieauthors
Minahk CJ,Saavedra L,Sesma F,Morero Rdoi
10.1016/j.biochi.2004.10.013keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
181-6issue
2eissn
0300-9084issn
1638-6183pii
S0300-9084(04)00192-0journal_volume
87pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::The effects of DNA oligonucleotides complementary to potato spindle tuber viroid (PSTVd) on viroid infection were investigated. The oligonucleotides were used to form hybrids with PSTVd in the infection mixture. A 75% reduction of viroid infection was found when an oligonucleotide complementary to nucleotides 79-110 o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90026-o
更新日期:1993-01-01 00:00:00
abstract::The interactions between lactic acid bacteria and their phages are commercially significant. Current research has focused on the elucidation of the mechanisms and genetics of phage resistance. Phage resistance genes have been linked to plasmid DNA for Streptococcus lactis and Streptococcus cremoris, and preliminary st...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(88)90215-5
更新日期:1988-03-01 00:00:00
abstract::L-aromatic aminoacid decarboxylase has been purified more than thousand times from homogenates of rat brain, in several steps : centrifugation, DEAE-cellulose, CM cellulose, hydroxylapatite, DEAE sephadex. Its properties have been studied, most of them on an intermediate fraction of the purification, because of the in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80356-7
更新日期:1975-01-01 00:00:00
abstract::Triple-negative breast cancer (TNBC) is a heterogeneous group of breast cancer with one common feature: distinctly metastatic nature with higher rate of relapse and shorter survival compared with other subtypes of breast cancer. The epithelial to mesenchymal transition (EMT) is highly associated with cancer metastasis...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.03.004
更新日期:2019-07-01 00:00:00
abstract::The regulation of transcription of the pckA gene coding for phosphoenolpyruvate carboxykinase in Escherichia coli was analysed by site-directed mutagenesis of the promoter region and measurement of in vitro transcription initiation. Mutation of the guanine residue at position -14 to either cytosine or thymine was foun...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80052-2
更新日期:1999-03-01 00:00:00
abstract::The Rap family of small GTP-binding proteins is composed by four different members: Rap1A, Rap1B, Rap2A and Rap2B. In this work we report the identification and characterization of a fifth member of this family of small GTPases. This new protein is highly homologous to Rap2A and Rap2B, binds labeled GTP on nitrocellul...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.08.007
更新日期:2006-03-01 00:00:00
abstract::Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine biosynthetic pathway and has been exploited as the target for therapy against proliferative and parasitic diseases. In this study, we report the crystal structures of DHODH from Leishmania major, the species of Leishmania associated wi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.04.003
更新日期:2012-08-01 00:00:00
abstract::Oligonucleotides are multifunctional molecules which can interfere with gene expression by different mechanism such as antisense, RNA interference, ribozymes, etc. For most in vivo diagnostic and therapeutic applications, oligonucleotides need to be delivered to the intracellular compartment of a specific organ, a dif...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2005.04.013
更新日期:2005-09-01 00:00:00
abstract::The vasoactive hormone bradykinin, its N-and C-terminal fragments and some structural analogues were studied by Circular Dichroism. Conformational features of the peptide can be detected by comparative analysis of the various CD spectra recorded as a function of aqueous pH, solvent and temperature. It is shown that th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80316-8
更新日期:1979-01-01 00:00:00
abstract::Canine S100A12 (cS100A12) is a calcium-binding protein of the S100 superfamily of EF-hand proteins, and its expression is restricted to neutrophils and monocytes. Interaction of S100A12 with the receptor for advanced glycation end products (RAGE) has been suggested to play a central role in inflammation. Moreover, S10...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.08.007
更新日期:2010-12-01 00:00:00
abstract::In order to identify conserved potential secondary structures within translational start sites, mRNA sequences derived from different species were studied with programs able to depict such features. The potential secondary structure of 71 bases around the initiator AUG or AUGs in the coding sequences of 290 eukaryotic...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90120-1
更新日期:1994-01-01 00:00:00
abstract::The hepatitis E virus (HEV) is the most common cause of acute hepatitis worldwide. Although HEV is a small, naked RNA virus, HEV particles become associated with lipids in the blood of infected patients and in the supernatant of culture systems. The egress of these particles from cells implies the exocytosis pathway b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.05.003
更新日期:2017-10-01 00:00:00
abstract::Homocysteine (HCY) is a degradation product of the methionine pathway. The B vitamins, in particular vitamin B12 and folate, are the primary nutritional determinant of HCY levels and therefore their deficiencies result in hyperhomocysteinaemia (HHCY). Prevalence of hyperhomocysteinemia (HHCY) and related dietary defic...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2016.04.009
更新日期:2016-07-01 00:00:00
abstract::Excessive tissue iron levels are associated with the increase of oxidative/nitrative stress which contributes to tissue damage that may elevate the risk of diabetes. Therefore, we investigated the effects of iron on diabetes-associated liver injury and whether iron-related tyrosine nitration participated in this proce...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.07.019
更新日期:2012-12-01 00:00:00
abstract::Phosphoenolpyruvate carboxykinase (PEPCK) is the key enzyme in glyceroneogenesis, an important metabolic pathway that functions to restrain the release of non-esterified fatty acids (NEFAs) from adipocytes. The antidiabetic drugs known as thiazolidinediones (TZDs) are thought to achieve some of their benefits by lower...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(01)01343-8
更新日期:2001-10-01 00:00:00
abstract::Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.06.009
更新日期:2012-11-01 00:00:00
abstract::High plasma choline has been associated with the metabolic syndrome and risk of chronic diseases, including cardiovascular disease. However, dietary choline is not correlated with choline plasma concentrations, and there are few studies and contradictory evidence regarding dietary choline and cardiovascular events. In...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.11.001
更新日期:2020-06-01 00:00:00
abstract::Experimental evidence accumulated to date by several research groups indicates that antisense oligodeoxynucleotides targeted against messenger RNA (mRNA) sequences located downstream of the initiation codon fail to inhibit the translation of this mRNA unless the hybrid is cleaved by RNase H. It has previously been sho...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90032-n
更新日期:1993-01-01 00:00:00
abstract::The interactions of DNA with histone H4 and with its fragments N-H4 (1-84) and C-H4 (85-102) have been studied by using electrooptical techniques, viscosity and electron microscopy. Electron microscopy reveals that histone H4 induces a large folding of DNA molecules : this is in agreement with electrooptical measureme...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80398-1
更新日期:1980-01-01 00:00:00
abstract::Ton-dependent colicins and microcins are actively taken up into sensitive cells at the expense of energy which is provided by the proton motive force of the cytoplasmic membrane. The Ton system consisting of the proteins TonB, ExbB and ExbD is required for colicin and microcin import. Colicins as well as the outer mem...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(02)01427-x
更新日期:2002-05-01 00:00:00
abstract::The effect of cycloheximide on rRNA and poly (A) (+) RNA synthesis is studied in radish seedlings. When used at a concentration of 50 microng/ml cycloheximide selectively blocks rRNA synthesis without altering poly (A) (+) RNA synthesis. Processing of the 2.3 X 10(6) dalton pre-rRNA is severely impaired. This observat...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80085-0
更新日期:1977-01-01 00:00:00
abstract::Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(92)90125-x
更新日期:1992-03-01 00:00:00
abstract::The concept of a stem cell subpopulation as understood from normal epithelial tissue or bone marrow function has been extended to our understanding of cancer tissue and is now the target of treatment efforts specifically directed to this subpopulation. In glioblastoma, as well as in other cancers, increased expression...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.11.007
更新日期:2013-04-01 00:00:00
abstract::It is now over 10 years since the discovery of peroxisome proliferator activated receptor gamma (PPAR gamma) and its unique role in adipogenesis. The subsequent identification of PPAR gamma as the target of insulin sensitizing drugs certified this ligand-regulated transcription factor as an exciting link between adipo...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2004.10.021
更新日期:2005-01-01 00:00:00
abstract::Peroxisomes are subcellular organelles which are present in virtually every eukaryotic cell and catalyze a large number of metabolic functions. The importance of peroxisomes for humans is stressed by the existence of a large group of genetic diseases in which either the biogenesis of peroxisomes is impaired or one of ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2013.08.022
更新日期:2014-03-01 00:00:00
abstract::Antibodies to Guo-AF were elicited in rabbits by immunization with bovine serum albumin -Guo-AF conjugate. The antibodies were purified by affinity chromatography. The affinity of the antibodies towards several ligands was studied by radioimmunoassays. The antibodies recognize Gua-AAF and Guo-AF (K(Guo-AF)/K(Guo-AAF) ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80066-6
更新日期:1980-01-01 00:00:00
abstract::The G protein coupled receptors (GPCR) are an important class of proteins that act as signal transducers through the cytoplasmic membrane. Understanding the structure and activation mechanism of these proteins is crucial for understanding many different aspects of cellular signalling. The olfactory receptors correspon...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(98)80019-9
更新日期:1998-02-01 00:00:00
abstract::Sticholysin II (StII) is a pore-forming toxin of biomedical interest that belongs to the actinoporin protein family. Sticholysins are currently under examination as an active immunomodulating component of a vaccinal platform against tumoral cells and as a key element of a nucleic acids delivery system to cell cytosol....
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.02.006
更新日期:2018-05-01 00:00:00
abstract::Experimental Lyme arthritis provides a mouse model for exploring the development of pathology following infection of C3H mice with Borrelia burgdorferi. Infected mice develop a reliable inflammatory arthritis of the ankle joint with severity that typically peaks around two to three weeks post-infection and then underg...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.06.010
更新日期:2017-10-01 00:00:00
abstract::Lactic acid bacteria provide a model system for the in vivo study of mechanisms pertaining to the regulation of sugar transport and metabolism by microorganisms. Recent studies with resting and growing cells of the homofermentative Streptococci and Lactobacilli have yielded evidence for hitherto unsuspected regulatory...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(88)90205-2
更新日期:1988-03-01 00:00:00