Abstract:
:Targeted gene transduction to specific tissues and organs through intravenous injection would be the ultimate preferred method of gene delivery. Here, we report successful targeting in a living animal through intravenous injection of a lentiviral vector pseudotyped with a modified chimeric Sindbis virus envelope (termed m168). m168 pseudotypes have high titer and high targeting specificity and, unlike other retroviral pseudotypes, have low nonspecific infectivity in liver and spleen. A mouse cancer model of metastatic melanoma was used to test intravenous targeting with m168. Human P-glycoprotein was ectopically expressed on the surface of melanoma cells and targeted by the m168 pseudotyped lentiviral vector conjugated with antibody specific for P-glycoprotein. m168 pseudotypes successfully targeted metastatic melanoma cells growing in the lung after systemic administration by tail vein injection. Further development of this targeting technology should result in applications not only for cancers but also for genetic, infectious and immune diseases.
journal_name
Nat Medjournal_title
Nature medicineauthors
Morizono K,Xie Y,Ringpis GE,Johnson M,Nassanian H,Lee B,Wu L,Chen ISdoi
10.1038/nm1192keywords:
subject
Has Abstractpub_date
2005-03-01 00:00:00pages
346-52issue
3eissn
1078-8956issn
1546-170Xpii
nm1192journal_volume
11pub_type
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