In vivo accumulation of different hypericin ion pairs in the urothelium of the rat bladder.

Abstract:

OBJECTIVE:To optimise the diagnostic and phototherapeutic efficacy of hypericin in superficial bladder cancer, by developing a bladder instillation fluid that does not depend on the presence of plasma proteins for an appropriate and reliable urothelial uptake of hypericin. MATERIALS AND METHODS:Sodium hypericinate (in distilled water, in sodium phosphate buffer, or in polyethylene glycol) and several other hypericinate salts (potassium, lysine, TRIS or hexylamine) were instilled with no plasma constituents into the rat bladder. The accumulation of hypericin was assessed with fluorescence microscopy. RESULTS:The diagnostic and phototherapeutic efficacy of hypericin depends on its ability to penetrate the tumour lesions sufficiently to show a fluorescent signal or elicit a photodynamic response. Several instillation fluids meet the purpose, as the urothelial accumulation of hypericin was similar to that obtained with the instillation fluid supplemented with plasma proteins, used in clinical practice. The highest concentrations of hypericin in the urothelium of the rat bladder were obtained with hypericin instillation solutions prepared with distilled water or 20% polyethylene glycol 400 in distilled water. Fluorescence microscopy showed that hypericin was selectively localized in the urothelium. Furthermore, all variables investigated (hydrophilic/lipophilic balance, pH, saline, presence of organic solvent) can dramatically influence the in vivo accumulation of hypericin. CONCLUSION:An appropriate and reliable urothelial uptake of hypericin does not depend on the presence of plasma protein supplements in the bladder instillation fluid.

journal_name

BJU Int

journal_title

BJU international

authors

Huygens A,Kamuhabwa AR,van Cleynenbreugel B,van Poppel H,Roskams T,de Witte PA

doi

10.1111/j.1464-410X.2005.05316.x

keywords:

subject

Has Abstract

pub_date

2005-02-01 00:00:00

pages

436-41

issue

3

eissn

1464-4096

issn

1464-410X

pii

BJU5316

journal_volume

95

pub_type

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