Abstract:
:Heat shock proteins (HSP's) closely interact with 20S proteasome and have been shown to maintain catalytic activity, responsible for the prevention of protein aggregation. A decrease in both proteasome activity and heat shock proteins (HSP's) has been observed with age. We investigated whether life-long calorie restriction (CR), a natural intervention, which prolongs life span, could prevent the age-associated decline in HSP's and restore the proteolytic activity of the 20S proteasome in skeletal muscle. Hence, we investigated HSP's and proteasome activity in the soleus muscle from 12-mo-old (Adult) and 26-28 mo old ad libitum fed (Old), and 26-28 mo old CR (Old-CR; fed 40% of ad libitum for their lifespan) male Fisher 344 rats. Trypsin-like proteasome activity in Old rats was significantly less than both Adult and Old-CR rats. Furthermore, no significant changes where found in chymotrypsin-like proteasome activity due to age or diet. Levels of HSP 72 and 25 were significantly less in Old animals when compared to both Adult and Old-CR rats. In contrast, HSP 90 was elevated in Old rats by 220% compared to adult animals and life-long calorie restriction caused a significant induction (150%) compared to age-matched ad libitum fed animals. Protein carbonyls were significantly elevated in Old when compared to Adult rats, but showed no significant decline due to life long CR. This study shows that HSP's may be largely responsible for the restoration of the trypsin-like activity of the 20S proteasome with age. The large increase in HSP 90 is intriguing and further studies are required to elucidate its role in maintaining 20S proteasome function.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Selsby JT,Judge AR,Yimlamai T,Leeuwenburgh C,Dodd SLdoi
10.1016/j.exger.2004.08.012keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
37-42issue
1-2eissn
0531-5565issn
1873-6815pii
S0531-5565(04)00267-0journal_volume
40pub_type
杂志文章abstract::To study the several elements and causes of ageing, diverse model organisms and methodologies are required. The most frequently used models are Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster and rodents. All have their advantages and disadvantages and allow studying particular aspects of the...
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pub_type: 杂志文章
doi:10.1016/s0531-5565(99)00087-x
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1016/0531-5565(84)90011-1
更新日期:1984-01-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2004.08.016
更新日期:2004-11-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 评论,杂志文章
doi:10.1016/j.exger.2016.08.008
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journal_title:Experimental gerontology
pub_type: 杂志文章
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2007.06.009
更新日期:2008-02-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2004.07.002
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pub_type: 杂志文章
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