Abstract:
:Manganese superoxide dismutase (MnSOD) levels have been found to be low in human pancreatic cancer [Pancreas 26, (2003), 23] and human pancreatic cancer cell lines [Cancer Res. 63, (2003), 1297] when compared to normal human pancreas. We hypothesized that stable overexpression of pancreatic cancer cells with MnSOD cDNA would alter the malignant phenotype. MIA PaCa-2 cells were stably transfected with a pcDNA3 plasmid containing sense human MnSOD cDNA or containing no MnSOD insert by using the lipofectAMINE method. G418-resistant colonies were isolated, grown and maintained. Overexpression of MnSOD was confirmed in two selected clones with a 2-4-fold increase in MnSOD immunoreactive protein. Compared with the parental and neo control cells, the MnSOD-overexpressing clones had decreased growth rates, growth in soft agar and plating efficiency in vitro, while in vivo, the MnSOD-overexpressing clones had slower growth in nude mice. These results suggest that MnSOD may be a tumor suppressor gene in human pancreatic cancer.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Ough M,Lewis A,Zhang Y,Hinkhouse MM,Ritchie JM,Oberley LW,Cullen JJdoi
10.1080/10715760400017376keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
1223-33issue
11eissn
1071-5762issn
1029-2470pii
RH5G98AD2VC6D5E8journal_volume
38pub_type
杂志文章abstract::Silibinin, a major active constituent of silymarin, is clinically used as a hepatoprotectant, and in recent years, it has been used for the treatment of cancer in China. Because the mechanism of silibinin action on cancer cells was still unclear, we investigated the contribution of silibinin to the induction of apopto...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.715369
更新日期:2012-11-01 00:00:00
abstract::The beneficial effects of hyperoxia have been noted in treatment of several diseases and pathological states. However, the excessive production of ROS under hyperoxic conditions can directly damage cellular macromolecules if the imbalance in antioxidant status exists. Cytochrome P450 (Cyp) 4a14 has an important role i...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715760903390820
更新日期:2010-02-01 00:00:00
abstract::Polystyrene supported fluorinated cyclic nitrone spin-traps: Resin-2-HFDMPO (2-hydroxymethyl-2-methyl-5-(trifluoromethyl)-3,4-dihydro-2H-pyrrole-1-oxide) and Resin-2-PFDMPO (2-(3-hydroxypropyl)-2-methyl-5-(trifluoromethyl)-3,4-dihydro-2H-pyrrole 1-oxide) containing a trifluoromethyl pyrroline-N-oxide core were develop...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1683171
更新日期:2019-12-01 00:00:00
abstract::It has been reported that haem oxygenase-1 (Hmox1) induction attenuates neointimal thickening. Here we further investigated the potential mechanisms regulating this important pathological process. We revealed that histone deacetylase 2 (HDAC2) was induced following Hmox1 induction under 1% oxygen treatment and this in...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1524578
更新日期:2018-10-01 00:00:00
abstract::Flavonoids are components of fruit and vegetables that may be beneficial in the prevention of disease such as cancer and cardiovascular diseases. Their beneficial effects will be dependent upon their uptake and disposition in tissues and cells. The metabolism and pharmacokinetics of flavonoids has been an area of acti...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760410001727858
更新日期:2004-08-01 00:00:00
abstract::The increasing knowledge on the participation of free radicals in many diverse clinical and pathological conditions, has consequently expanded the search for new and versatile antioxidants aimed at combating oxidative stress. Our interest in this field concerns aromatic indolinonic aminoxyls (nitroxides) which efficie...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900301621
更新日期:1999-08-01 00:00:00
abstract::There is clear evidence implicating oxidative stress in the pathology of many neurodegenerative diseases. Reactive oxygen species (ROS) are the primary mediators of oxidative stress, and hydrogen peroxide, a key ROS, is generated during aggregation of the amyloid proteins associated with some of these diseases. Hydrog...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600632545
更新日期:2006-07-01 00:00:00
abstract::Oxidative stress is associated with a range of inflammatory lung diseases including asthma, adult respiratory distress syndrome, idiopathic pulmonary fibrosis, pneumonia, lung transplantation, chronic obstructive pulmonary disease, cystic fibrosis, bronchiectasis and lung cancer. Increased concentrations of reactive o...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760310001623331
更新日期:2003-12-01 00:00:00
abstract::Myeoloperoxidase catalyses the formation of hypochlorous acid (HOCl) via reaction of H(2)O(2) with Cl(-) ion. Although HOCl is known to play a major role in the human immune system by killing bacteria and other invading pathogens, excessive generation of this oxidant is known to cause damage to tissue. Recently, it wa...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802098834
更新日期:2008-05-01 00:00:00
abstract::Effects of vitamins B, C, E, K and P, as well as coenzymes Q, on formation of final products of radiation-induced free-radical transformations of ethanol, ethylene glycol, alpha-methylglycoside and glucose in aqueous solutions were studied. Based on the obtained results, it can be concluded that there are substances a...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500147479
更新日期:2005-07-01 00:00:00
abstract::Non-small cell lung cancer (NSCLC) is the most common form of lung cancer. Radioresistance is a significant obstacle in NSCLC radiotherapy. Long non-coding RNA LINC00473 has been found to impact the radiotherapy in several malignant tumours. This study aimed to investigate the underlying role and mechanism of LINC0047...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2020.1841900
更新日期:2020-10-01 00:00:00
abstract::The aim of the study was to analyse the effect of Val 16Ala polymorphism in SOD2 gene on oxidative stress parameters and lipid profile of the blood during a three-month wrestling training. The study included 53 Polish young wrestlers. Blood samples were collected at the beginning of the programme and following three m...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1327716
更新日期:2017-05-01 00:00:00
abstract::Neuroblastoma (NB) is one of the most frequent extracranial solid tumors in children. It accounts for 8-10% of all childhood cancer deaths, and there is a need for development of new drugs for its treatment. Curcumin (diferuloylmethane), a major active component of turmeric (Curcuma longa), has been shown to exert ant...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.960410
更新日期:2014-12-01 00:00:00
abstract::Nine male volunteers were given 36 g of cocoa powder (containing 2610 mg of polyphenols) per day with sugar and 6 volunteers received an equivalent amount of sugar for 2 weeks. Conjugated diene production in LDL induced by 2-2' azobis(4-methoxy-2,4-dimethylvaleronitrile) (V-70) and copper ion were evaluated. The lag t...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300091
更新日期:2001-01-01 00:00:00
abstract::Proteins are subject to various posttranslational modifications, some of them being undesired from the point of view of metabolic efficiency. Prevention of such modifications is expected to provide new means of therapy of diseases and decelerate the process of aging. In this review, modifications of proteins by reacti...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2014.953494
更新日期:2014-11-01 00:00:00
abstract::Lipid peroxidation (LPO) product accumulation in human tissues is a major cause of tissular and cellular dysfunction that plays a major role in ageing and most age-related and oxidative stress-related diseases. The current evidence for the implication of LPO in pathological processes is discussed in this review. New d...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2010.498478
更新日期:2010-10-01 00:00:00
abstract::Gastric inflammation is a highly complex biochemical protective response to cellular/tissue injury. When this process occurs in an uncontrolled manner, the result is excessive cellular/tissue damage that results chronic inflammation and destruction of normal tissue. Current evidence suggests that Helicobacter pylori (...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760000301301
更新日期:2000-12-01 00:00:00
abstract::To examine the involvement of p38 mitogen-activated protein kinase (p38 MAPK) and extracellular signal-regulated kinase (ERK) in the oxidative stress-induced increase of permeability in endothelial cells, the effects of a p38 MAPK inhibitor (SB203580) and ERK inhibitor (PD90859) on the H2O2-induced increase of permeab...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100301531
更新日期:2001-11-01 00:00:00
abstract::Oxidative stress has been consistently linked to ageing-related neurodegenerative diseases. Neurodegenerative diseases are characterized by progressive dysfunction and death of neurons. Oxidative stress is associated with dysfunction of the mitochondria and endoplasmic reticulum, inducing apoptosis and protein misfold...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2011.574290
更新日期:2011-08-01 00:00:00
abstract::Diosgenin possesses anti-inflammatory and anticancer properties. Activated neutrophils produce high concentrations of the superoxide anion which is involved in the pathophysiology of inflammation-related diseases and cancer. In the present study, the inhibitory effect and possible mechanisms of diosgenin on superoxide...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.966705
更新日期:2014-12-01 00:00:00
abstract::The potential protective effect of the dietary antioxidant curcumin (120 mg/Kg/day for 6 days) against the renal injury induced by maleate was evaluated. Tubular proteinuria and oxidative stress were induced by a single injection of maleate (400 mg/kg) in rats. Maleate-induced renal injury included increase in renal v...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.954109
更新日期:2014-11-01 00:00:00
abstract::Gamma-glutamylcysteine synthetase (GCS; also referred to as glutamate-cysteine ligase, GLCL) catalyzes the rate-limiting reaction in glutathione (GSH) biosynthesis. The GCS holoenzyme is composed of a catalytic and regulatory subunit, each encoded by a unique gene. In addition to some conditions which specifically upr...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760000300291
更新日期:2000-04-01 00:00:00
abstract::Peroxynitrite anion is a reactive and short-lived species and its formation in vivo has been implicated in several human diseases. In view of the potential usefulness of compounds that can protect against peroxynitrite or their reactive intermediates, a study focused on flavonoid compounds was carried out. Since the r...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400003384
更新日期:2004-09-01 00:00:00
abstract::This experiment was performed to clarify the role of protein kinase C (PKC) delta in NADPH oxidase-dependent O(2-) production and actin polymerization followed by phagocytosis in neutrophils. Bovine neutrophils and human neutrophil-like differentiated HL-60 (dHL-60) cells were stimulated with serum-opsonized zymosan (...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500539121
更新日期:2006-04-01 00:00:00
abstract::Retinal mitochondria become dysfunctional in diabetes and the production of superoxide radicals is increased; over-expression of MnSOD abrogates mitochondrial dysfunction and prevents the development of diabetic retinopathy. The mitochondrial DNA (mtDNA) is particularly prone to oxidative damage. The aim of this study...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715760903494168
更新日期:2010-03-01 00:00:00
abstract::A zinc(II) phthalocyanine compound, tetra-alpha-(2,2,4-tirmethyl-3-pentoxy) Phthalocyanine Zinc (ZnPc(OR)(4)) was synthesized in this paper and this zinc (II) phthalocyanine compound was used as the photosensitizer in the photoinduced chemiluminescence (PCL) of lucigenin in N,N-dimethylformamide (DMF). The photoexcite...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701836839
更新日期:2008-02-01 00:00:00
abstract::We have reported previously that the apparent rate of peroxynitrite (ONOO(-) ) decay, as followed from its absorbance at 302 nm, decreases in the presence of hydrogen peroxide, mannitol and ethanol (Alvarez et al., 1995, Chem. Res. Toxicol. 8:859-864; Alvarez et al., 1998, Free Radic. Biol. Med. 24:1331-1337). Recentl...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300411
更新日期:2001-05-01 00:00:00
abstract::Lectin-like oxidized low-density lipoprotein (LOX-1) has been identified in endothelial cells as the main receptor of oxidized low-density lipoprotein (OxLDL). LOX-1 is upregulated in the presence of pathological conditions including atherosclerosis, hypertension, and diabetes because it acts as a mediator of "endothe...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2014.929122
更新日期:2014-08-01 00:00:00
abstract::Araucaria angustifolia (Bert.) O. Kuntze is a species critically endangered of extinction and its development and propagation is strongly affected by abiotic stress. We have previously shown the activation of uncoupling protein in A. angustifolia embryogenic stem cells subjected to cold stress. Now, we have furthered ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1548767
更新日期:2019-01-01 00:00:00
abstract::Hemopexin, a heme-binding serum glycoprotein, is thought to play an important role in the prevention of oxidative damage that may be catalysed by free heme. Through the use of EPR techniques, the generation of free radicals from organic hydroperoxides by heme and heme-hemopexin complexes, and the concomitant formation...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509065277
更新日期:1995-12-01 00:00:00