Abstract:
:Cell replacement therapy is of great interest as a long-term treatment of neurodegenerative diseases such as Parkinson's disease (PD). We have previously shown that Sertoli cells (SC) provide neurotrophic support to transplants of dopaminergic fetal neurons and NT2N neurons, derived from the human clonal precursors cell line NTera2/D1 (NT2), which differentiate into dopaminergic NT2N neurons when exposed to retinoic acid. We have created SC-NT2 cell tissue constructs cultured in the high aspect ratio vessel (HARV) rotating wall bioreactor. Sertoli cells, NT2, and SC plus NT2 cells combined in starting ratios of 1:1, 1:2, 1:4 and 1:8 were cultured in the HARV in DMEM with 10% fetal bovine serum and 1% growth factor reduced Matrigel for 3 days, without retinoic acid. Conventional, non-HARV, cultures grown in the same culture medium were used as controls. The presence of tyrosine hydroxylase (TH) was assessed in all culture conditions. Sertoli-neuron-aggregated-cell (SNAC) tissue constructs grown at starting ratios of 1:1 to 1:4 contained a significant amount of TH after 3 days of culture in the HARV. No TH was detected in SC HARV cultures, or SC, NT2 or SC-NT2 conventional co-cultures. Quantitative stereology of immunolabled 1:4 SNAC revealed that approximately 9% of NT2 cells differentiate into TH-positive (TH+) NT2N neurons after 3 days of culture in the HARV, without retinoic acid. SNAC tissue constructs also released dopamine (DA) when stimulated with KCl, suggesting that TH-positive NT2N neurons in the SNAC adopted a functional dopaminergic phenotype. SNAC tissue constructs may be an important source of dopaminergic neurons for neuronal transplantation.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Saporta S,Willing AE,Shamekh R,Bickford P,Paredes D,Cameron DFdoi
10.1016/j.brainresbull.2004.09.003keywords:
subject
Has Abstractpub_date
2004-12-15 00:00:00pages
347-56issue
4eissn
0361-9230issn
1873-2747pii
S0361-9230(04)00223-0journal_volume
64pub_type
杂志文章abstract::Rats were unilaterally deprived of odor stimulation by surgically sealing one naris 1, 30 or 60 days after birth. Thirty days later the laminar volumes of the olfactory bulb ipsilateral to the occluded naris were compared with those ipsilateral to the normal side. Deprivation from days 1-30 resulted in approximately 2...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(83)90121-1
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journal_title:Brain research bulletin
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journal_title:Brain research bulletin
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Brain research bulletin
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journal_title:Brain research bulletin
pub_type: 杂志文章
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journal_title:Brain research bulletin
pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
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pub_type: 杂志文章
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journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(89)90212-8
更新日期:1989-10-01 00:00:00
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journal_title:Brain research bulletin
pub_type: 杂志文章,评审
doi:10.1016/j.brainresbull.2016.06.016
更新日期:2016-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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journal_title:Brain research bulletin
pub_type: 杂志文章,评审
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更新日期:2011-03-10 00:00:00
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journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/s0361-9230(96)00199-2
更新日期:1996-01-01 00:00:00
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journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/j.brainresbull.2007.07.012
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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journal_title:Brain research bulletin
pub_type: 杂志文章
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更新日期:2008-06-15 00:00:00
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journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(91)90275-o
更新日期:1991-07-01 00:00:00
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journal_title:Brain research bulletin
pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2021-01-10 00:00:00
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journal_title:Brain research bulletin
pub_type: 杂志文章
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更新日期:2008-07-30 00:00:00
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pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00
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journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/j.brainresbull.2004.01.011
更新日期:2004-04-30 00:00:00
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journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(84)90133-3
更新日期:1984-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.brainresbull.2011.05.009
更新日期:2011-07-15 00:00:00