Abstract:
:We applied a strategy that utilized a combination of systematic gene expression analysis with various tissues and immunological detection with sera from melanoma patients to identify melanoma antigens expressed preferentially in melanoma and melanocytes. We selected 101 genes by comparing cDNA profiles obtained by GeneChip analysis of a highly pigmented melanoma cell line, SKmel23, primary cultured melanocytes, HUVECs cultured endothelial cells, keratinocytes, liver and stomach. After the additional selection with criterion of high registered frequency of each cDNA in melanocyte-related cDNA libraries in the NCBI database, 15 genes including 12 known melanocyte specific genes were identified. One of the remaining 3 genes, FCRL/FREB, encoding a member of the Fc receptor family that was previously reported to express in germinal center B cells, was found to express preferentially in melanocytes and melanoma tissues by RT-PCR and Northern blot analysis. The FCRL/FREB protein was detected in the cytoplasm of melanoma cells by staining with the murine polyclonal antibody and by transfection with GFP-fused FCRL/FREB cDNA. The bacterial recombinant protein was recognized by serum IgG antibody obtained from some patients with melanoma. These results suggest that FCRL/FREB may function in melanocytes and melanoma and may be useful for development of diagnostic methods for various pigment disorders and immunotherapy of melanoma.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Inozume T,Matsuzaki Y,Kurihara S,Fujita T,Yamamoto A,Aburatani H,Shimada S,Kawakami Ydoi
10.1002/ijc.20735keywords:
subject
Has Abstractpub_date
2005-03-20 00:00:00pages
283-90issue
2eissn
0020-7136issn
1097-0215journal_volume
114pub_type
杂志文章abstract::Expression levels of hTERT mRNA were investigated by RT-PCR in tissue specimens of patients with (Group A) and without (Group B) clinically overt bronchial carcinoma, respectively. Bronchial carcinoma (n = 9) and distant normal (n = 9) specimens were analyzed in Group A. The chance of carcinoma seemed to increase with...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11732
更新日期:2004-04-10 00:00:00
abstract::Because renal-cell carcinoma (RCC) is considered relatively resistant to radio- and chemotherapy, RCC patients may benefit from new treatment modalities, e.g. immunotherapy. In vitro and in vivo studies suggest that combinations of cytokines such as interferon gamma or interferon alpha (IFN-gamma, IFN-alpha) and tumor...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910560220
更新日期:1994-01-15 00:00:00
abstract::Benzo(a)pyrene and benz(a) anthrancene which, in contrast to the K-region epoxides benzo(a)pyrene 4,5-oxide and benz(a)anthracene 5,6-oxide, are not mutagenic to Salmonella typhimurium TA 1537 in the absence of mammalian enzyme preparations, were activated by liver microsomes from C3H mice, which had not received any ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910180408
更新日期:1976-10-15 00:00:00
abstract::The calcium-sensing receptor (CaSR) is suggested to mediate the antiproliferative effects of calcium in colon. However, in colorectal cancer (CRC) the expression of the CaSR is silenced and the underlying mechanisms leading to its loss are poorly understood. We investigated whether loss of the CaSR expression in color...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28856
更新日期:2014-11-01 00:00:00
abstract::The natural history of human T-lymphotropic virus type I (HTLV-I) has been shown to differ markedly by geographic area. The differences include contrasting patterns of risk of adult T-cell lymphoma (ATL) and HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP), which may be due in part to differences in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24012
更新日期:2009-02-01 00:00:00
abstract::A panel of conventional and monoclonal antibodies was used to examine the immunohistological characteristics of malignant epithelial cells and infiltrating lymphocytes in frozen sections of nasopharyngeal carcinoma (NPC) biopsies from 10 Tunisian patients. Three main categories of cells were identified. (1) Tumour cel...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910330616
更新日期:1984-06-15 00:00:00
abstract::Recently, pegylated arginine deiminase (ADI; EC 3.5.3.6) has been used to treat the patients with hepatocellular carcinoma or melanoma, in which the level of argininosuccinate synthetase (ASS) activity is low or undetectable. The efficacy of its antitumor activity largely depends on the level of intracellular ASS, whi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22322
更新日期:2007-02-15 00:00:00
abstract::Colorectal cancer remains a leading cause of cancer death worldwide, despite markedly improved response rates to current systemic therapies. Oxaliplatin either alone or incorporated into 5-fluorouracil/leucovorin regimes has resulted in increased survival rates, particularly with regards to metastatic colorectal carci...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22645
更新日期:2007-07-01 00:00:00
abstract::In colorectal cancer (CRC), hepatic arterial infusion (HAI) chemotherapy may convert primarily unresectable CRC liver metastases (CLM) into resectability, although the risk of metastatic recurrence remains high after CLM ablation. We investigated the role of antitumour immunity invoked by first-line oxaliplatin-HAI fo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32847
更新日期:2020-04-01 00:00:00
abstract::Loss of heterozygosity on chromosome arm 16q frequently occurs in human breast carcinomas regardless of the histological grade or type. To reveal whether the status of chromosome 16 corresponds to the histology of breast carcinomas, we examined the signal number of 16cen, 16q breakpoints and 1;16 fusions in the interp...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990820)84:4<381::aid-ijc
更新日期:1999-08-20 00:00:00
abstract::Epidemiological studies of in situ breast cancer are sparse, and the role of reproductive history, an established risk modifier for invasive breast cancer, remains incompletely investigated. To examine possible associations with parity and age at first birth, we undertook a case-control study nested in a nationwide co...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980729)77:3<330::aid-ijc
更新日期:1998-07-29 00:00:00
abstract::Alterations of gene expression in squamous cell carcinoma (SCC) cell lines derived from the larynx and keratinocytes derived from adjacent normal mucosa of the larynx have been studied using the mRNA differential display technique. Lane-to-lane comparison of reverse transcribed mRNA showed a strong repression of a 148...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19991210)83:6<750::aid-ijc
更新日期:1999-12-10 00:00:00
abstract::Genetic and epigenetic alterations during development of pancreatic ductal adenocarcinomas (PDACs) are well known. This study investigates genetic and epigenetic data together with tumor biology to find specific alterations responsible for metastasis formation. Using 16 human PDAC cell lines in a murine orthotopic PDA...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24695
更新日期:2010-01-01 00:00:00
abstract::Immunostimulatory DNA containing unmethylated cytosine-guanine (CpG) motifs have been successfully used as adjuvants to enhance the immunity of vaccines designed to trigger antitumor T-cell responses. We examined the effect of a CpG oligodeoxynucleotide (CpG ODN) for its ability to potentiate the activity of tumor ant...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24009
更新日期:2009-02-15 00:00:00
abstract::An immunopotentiating factor associated with spleen cells of C57BL/6J mice bearing the 3LL tumor is described. Supernatants of cultured spleen cells from tumor-bearing mice (TBM) augmented the generation of both 19S and 7S antibody-producing cells, when injected with sheep erythrocytes into syngeneic C57BL/6J mice. Th...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910220416
更新日期:1978-10-15 00:00:00
abstract::In order to define technical limitations of conventional external beam irradiation for clinically localized prostate cancer, we evaluated the impact of several reduced-field treatment factors, such as reduced-field (RF) irradiated volume, RF technique, photon energy of treatment, and dose on survival endpoints and loc...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-10-20 00:00:00
abstract::The recently described PGM-2 leukemia displays a hierarchical structure with bipotential stem cells, B-lymphocyte and macrophage progenitor cells, and post-mitotic end cells. Because the different cell types can easily be identified in vitro by clonal culture assays and simple staining procedures, this leukemia is a u...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910600515
更新日期:1995-03-03 00:00:00
abstract::An antiserum to SF antigen was shown to be selectively cytotoxic to two human fibroblast lines, while two tumor cell lines (one melanoma and one Hela cell line) were resistant. The selective cytotoxicity was also demonstrable in mixtures of fibroblasts and tumor cells. The cytotoxic effect was abrogated by admixture o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910180317
更新日期:1976-09-15 00:00:00
abstract::Family history of urogenital cancers has been associated with an increased risk of cancer at the same sites. To evaluate the risks of bladder, prostate and renal cell neoplasms for family history of urogenital cancers in first-degree relatives, we analysed data from a case-control study conducted in northern Italy bet...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.23037
更新日期:2007-12-15 00:00:00
abstract::A glycolipid extract was prepared from an SV40-transformed hamster cell line (EH-SV) according to the Folch partition procedure. The glycolipids from the aqueous layer were incorporated in liposomal membranes composed of lecithin/sphingomyelin/cholesterol (1:1:2 by weight). This liposomal preparation was inoculated in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910250616
更新日期:1980-06-15 00:00:00
abstract::The development of cervical carcinoma is closely associated with HPV infection. However, other genetic alterations also play an important role. In this study, we analyzed copy number alterations of several oncogene loci in a panel of 84 cervical tumors. Sixty-five (77%) tumors were HPV DNA-positive, and most were infe...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10627
更新日期:2002-10-10 00:00:00
abstract::To understand specific interactions between stromal cells and epithelial cells in benign prostatic hyperplasia (BPH) and prostatic adenocarcinoma, we developed stromal-cell cultures from normal human prostate (PNX) and BPH (BH101), composed of fibroblasts and myofibroblasts. Their role in epithelial-cell growth was st...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19961009)68:2<207::AID-IJC
更新日期:1996-10-09 00:00:00
abstract::Cytoskeleton and extracellular matrix-related proteins (CECMPs) represent the most common class of cancer mutants, owing to the large size of their coding regions and the randomness of mutagenesis. We used a bioinformatics approach to assess the impact of amino acid (AA) substitutions on the sensitivity of CECMPs to p...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31111
更新日期:2018-03-01 00:00:00
abstract::A population-based case-control study was conducted to evaluate risk of developing multiple myeloma (MM) associated with personal history of autoimmune diseases and occurrence of autoimmune and selected hematologic disorders in first-degree relatives. Data were obtained for all (n = 8,406) MM cases diagnosed in Sweden...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21745
更新日期:2006-06-15 00:00:00
abstract::Apoptosis protease-activating factor 1 (APAF-1), a transcriptional target of p53, is a cytosolic adaptor protein that links the mitochondrial apoptosis pathway to the caspase cascade. Here, we aimed to study the impact of APAF-1 expression levels on cell death induced by anticancer drugs or ionizing irradiation (IR) a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21535
更新日期:2006-05-01 00:00:00
abstract::In previous studies we showed that human sarcoma and melanoma cell lines synthesize and secrete into culture medium a glycoprotein, migrating in urea sodium dodecyl sulfate-polyacrylamide gel electrophoresis at Mr 140,000. It is not detected in cultures of the corresponding normal cells. Conditioned medium of the mela...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910370114
更新日期:1986-01-15 00:00:00
abstract::The relationship between renal-cell cancer (RCC) and occupation was investigated in an international multicenter population-based case-control study. Study centers in Australia, Denmark, Germany, Sweden and the United States interviewed 1732 incident RCC cases and 2309 controls. Significant associations were found wit...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.2910610503
更新日期:1995-05-29 00:00:00
abstract::Diabetes refers to a group of metabolic diseases characterized by impaired insulin signalling and high blood glucose. A growing body of epidemiological evidence links diabetes to several types of cancer but the underlying molecular mechanisms are poorly understood. The signalling cascade connecting insulin and FOXO pr...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.30840
更新日期:2017-12-15 00:00:00
abstract::Seven-day-old mouse embryos were transplanted under the kidney capsule of adult syngeneic recipients which were either X-irradiated, treated with cyclophosphamide, splenectomized or immunized with teratocarcinoma cell lines prior to embryonic transplantation. The yield of malignant tumors was decreased in preirradiate...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910300613
更新日期:1982-12-15 00:00:00
abstract::Salvicine is a diterpenoid quinone derived from a traditional Chinese medication that has been shown to possess potent in vitro and in vivo antitumor effects. This compound, which inhibits the activity of Topoisomerase II, was found to equipotently kill various multidrug-resistant tumor cells and their corresponding p...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20026
更新日期:2004-07-10 00:00:00