Phosphate increases mitochondrial reactive oxygen species release.

Abstract:

:The effects of inorganic phosphate (Pi), the main intracellular membrane permeable anion capable of altering mitochondrial pH gradients (Delta pH), were measured on mitochondrial H2O2 release. As expected, Pi decreased Delta pH and increased the electric membrane potential (Delta Psi). Mitochondrial H2O2 release was stimulated by Pi and also by its structural analogue arsenate. However, acetate, another membrane-permeable anion, did not stimulate mitochondrial H2O2 release. The stimulatory effect promoted by Pi was prevented by CCCP, which decreases transport of Pi across the inner mitochondrial membrane, indicating that Pi must be in the mitochondrial matrix to stimulate H2O2 release. In conclusion, we found that Pi and arsenate stimulate mitochondrial reactive oxygen release, an effect that may contribute towards oxidative stress under conditions such as ischemia/reperfusion, in which high-energy phosphate bonds are hydrolyzed.

journal_name

Free Radic Res

journal_title

Free radical research

authors

Oliveira GA,Kowaltowski AJ

doi

10.1080/10715760400009258

keywords:

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

1113-8

issue

10

eissn

1071-5762

issn

1029-2470

pii

7X7TW957AAJKLQ47

journal_volume

38

pub_type

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