Abstract:
:The nonmetabolizable glucose analogue [(3)H]-2-deoxy-D-glucose ((3)H-2DG) was used to study glucose transport in cultured rat podocytes. Intracellular accumulation of (3)H-2DG was linear up to 20 min and was inhibited by cytochalasin B (80% inhibition) and by phlorizin (20% inhibition). Pretreatment with insulin stimulated the (3)H-2DG uptake 1.5-fold. A Hill analysis of the rate of glucose transport yielded a V(max) value of approximately 10 mM and S(0.5)of 7.8 mM. The value h = 1.0 for a Hill coefficient confirmed that glucose uptake exhibited a Michaelis-Menten kinetics. Transporters GLUT2 and GLUT4 were expressed in over 90% podocytes. Of the GLUT2- and GLUT4-expressing cells, approximately one-fourth expressed the membrane-bound fraction. We conclude that cultured rat podocytes possess a differentiated glucose transport system consisting chiefly of facilitative GLUT2 and GLUT4 transporters. It seems likely that a sodium-dependent glucose cotransporter may also be present in these cells.
journal_name
Kidney Blood Press Resjournal_title
Kidney & blood pressure researchauthors
Lewko B,Bryl E,Witkowski JM,Latawiec E,Gołos M,Endlich N,Hähnel B,Koksch C,Angielski S,Kriz W,Stepinski Jdoi
10.1159/000080889keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
1-7issue
1eissn
1420-4096issn
1423-0143pii
80889journal_volume
28pub_type
杂志文章abstract:BACKGROUND/AIMS:Renal tubulointerstitium plays an important role in the development and progression of diabetic nephropathy. METHODS:With the present study, we aimed at evaluating the levels of neutrophil gelatinase-associated lipocalin (NGAL), a tubular stress protein, in serum (sNGAL) and urine (uNGAL) from a cohort...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000209379
更新日期:2009-01-01 00:00:00
abstract:INTRODUCTION:There is still no evidence whether human peritoneal mesothelial cells (HPMC) from patients with end-stage renal failure are altered in cell viability or show a different pattern of the release of proinflammatory cytokines. Also the serum of patients with uremia may contain substances stimulating the cytoki...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000066347
更新日期:2002-01-01 00:00:00
abstract::The high mortality rate seen in dialysis patients is related to long-standing high blood pressure and the presence of other traditional as well as non-traditional risk factors for cardiovascular disease. Hypertension is associated with increased risk for left ventricular hypertrophy, coronary artery disease, congestiv...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,评审
doi:10.1159/000070987
更新日期:2003-01-01 00:00:00
abstract::Since reports of the first data on cytokines in renal diseases about 10 years ago, the field has seen an explosive growth over the last decade. It is now realized that cytokines can be synthesized by many different types of cells, including glomerular cells, upon adequate stimulation and exert a wide range of actions ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,评审
doi:10.1159/000174088
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND AND AIM:Cinacalcet effectively reduces calcium in patients with persistent hyperparathyroidism (HPT) after kidney transplantation. We aimed to assess the association of cinacalcet with a decrease in renal function based on a meta-analysis of observational studies in kidney transplant patients with persistent...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,meta分析
doi:10.1159/000323902
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:There is limited evidence on the etiology and outcomes of renal infarction. A provoking factor is identified only in one- to two-thirds of patients. METHODS:This is a retrospective observational study. The clinical characteristics and outcomes of patients with acute renal infarction were studied; the sample...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000503425
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND/AIMS:Cardiorenal syndrome type 1 (CRS1) is a syndrome characterized by a rapid worsening of cardiac function leading to acute kidney injury (AKI). The aims of this study were to investigate the risk factors and the prognosis of CRS1 in elderly patients. METHODS:A total of 312 elderly patients (≥60 years old...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000447936
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND/AIM:Serum gamma-glutamyltransferase (GGT), a biomarker of oxidative stress, is associated with an increased risk of diabetes and hypertension. However, it is not known whether higher serum GGT is independently associated with chronic kidney disease (CKD). Therefore, we examined the association between serum ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000274463
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Urine osmolality indicates the ability of the kidney to concentrate the urine and reflects the antidiuretic action of vasopressin. However, results about the association between urine osmolality and adverse renal outcomes in chronic kidney disease (CKD) are conflicting. We investigated the association betwee...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000502291
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND/AIMS:Apparent treatment resistant hypertension (aTRH) is highly prevalent in patients with type 2 diabetes (T2D) and chronic kidney disease (CKD). The impact of aTRH and achievement of recommended blood pressure (BP) values on the rate of glomerular filtration rate (eGFR) loss in CKD patients is poorly known...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000488255
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Treating non-diabetic proteinuric patients with advanced renal disease with an angiotensin-converting enzyme (ACE) inhibitor is still subject to discussion. This study aims to determine the cost-effectiveness of ACE inhibitor therapy in this patient population in the Netherlands. METHODS:We compared two str...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000350142
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND/AIM:Our aim was to determine whether altered human β-defensin (HBD), pro-inflammatory cytokines including interleukin (IL)-6 and tumor necrotic factor (TNF)-α could increase the risk of developing and exacerbation of chronic kidney disease (CKD), especially for patients with diabetic nephropathy (DN). METHO...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000355740
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND/AIMS:Lung dysfunction associates with increased mortality but the impact of chronic kidney disease (CKD) is less clear. We evaluated lung function and its association with mortality among individuals with normal to severely reduced glomerular filtration rate (GFR). METHODS:404 individuals representing GFR c...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000488699
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Metabolic acidosis may be diagnosed as chronic (cMA) if it persists for at least 5 days, although an exact definition has not been provided by any guidelines yet. The most common cause is CKD; numerous less-known diseases can also account for cMA. SUMMARY:In recent years, CKD-associated cMA has been propose...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,评审
doi:10.1159/000510829
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND/AIMS:This study aimed to investigate the potential beneficial anti-proteinuric effect of an add-on aldosterone blockade and the impact of the aldosterone escape phenomenon. METHODS:We retrospectively analyzed data of 304 patients with persistent proteinuria, who were administered spironolactone (25 mg/day) ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000368470
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND/AIMS:Cardiovascular morbidity and mortality are high in patients with chronic kidney disease. We evaluated the influence of small differences in preoperative kidney function on mortality and complications following cardiac surgery. METHODS:This is an observational study that included adult patients undergoi...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000335950
更新日期:2012-01-01 00:00:00
abstract:AIMS:To investigate whether the regulation of aquaporin (AQP) channels is altered by inhibition of type 2 11beta-hydroxysteroid dehydrogenase (11betaHSD2). METHODS:Male Sprague-Dawley rats were treated with glycyrrhizic acid (GA, 2 g/l drinking water) for 7 days. The expression of AQP2 and AQP3 was determined in the k...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000098435
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:The biological properties of oxidized and non-oxidized PTH are substantially different. Oxidized PTH (oxPTH) loses its PTH receptor-stimulating properties, whereas non-oxidized PTH (n-oxPTH) is a full agonist of the receptor. This was described in more than 20 well published studies in the 1970(s) and 80(s)....
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000350149
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND/AIMS:The deposition of β2-microglobulin induced by reactive inflammation causing carpal tunnel syndrome (CTS) is one of the complications of dialysis-related amyloidosis in maintenance hemodialysis (MHD) patients. Air pollution levels, especially particulate matter with an aerodynamic diameter of <2.5 mm (PM...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000484422
更新日期:2017-01-01 00:00:00
abstract::A 70-year-old female patient with advanced Shy-Drager syndrome exhibited severe orthostatic hypotension, low serum catecholamine levels, and autonomic dysfunction. She was bedridden despite oral medication with fludrocortisone, etilefrin, dihydroergotamine, L-dopa, yohimbine, and amezinium methyl sulfate. Only intrave...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000025846
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND/AIMS:This experimental study was designed to investigate protective and therapeutic effects of Dexpanthenol (Dxp), an alcoholic analogue of pantothenic acid, on kidney damage induced by ischemia-reperfusion (I/R) in rats. METHODS:Forty rats were randomly divided into a control group and 4 I/R groups (1 h is...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000343411
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:In uremic patients coronary surgery and the entire perioperative management is demanding. METHODS:We analyzed retrospectively data from all patients requiring chronic hemodialysis who under went coronary artery bypass grafting (CABG) between January 1 2001 and December 31 2004 at the Deutsches Herzzentrum B...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000090180
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND/AIMS:We examined the factors associated with blood pressure variability in a multi-ethnicity prospective study in China to gain more evidence to guide the prevention and management of hypertension through risk factor intervention. METHODS:A total of 318 consecutive adult subjects aged 29-94 years with suspe...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000477424
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Post-transplant diabetes mellitus (PTDM) is a common and serious metabolic complication. Genetic polymorphisms of angiotensin-converting enzyme (ACE) and angiotensinogen (AGT) genes have been reported to be related to diabetes mellitus and insulin sensitivity; however, the role of these genes in the developm...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000343404
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Magnoline is an active ingredient of magnolia fargesii with anti-inflammatory and anti-platelet effects. The objective is to explore the renoprotection of magnoline in diabetic rats and its effects on P-selectin. METHODS:Thirty-six rats were randomized into 4 groups-normal control group (C), d...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000350146
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND/AIMS:Chronic renal allograft dysfunction (CRAD) is a leading cause of long-term renal allograft loss. Oxidative stress may account for the nonspecific interstitial fibrosis and tubular atrophy that occur in CRAD. An antioxidant intervention via Nrf2 signaling pathway activation might be a promising therapy f...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000487501
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND/AIMS:Kidney function is critical for homocysteine (Hcy) clearance, and plasma Hcy levels are frequently increased in patients with renal failure. Microalbuminuria (MAU) is an important marker of early renal damage caused by hypertension. At present, there is insufficient evidence on the relationship between ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000486013
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND/AIMS:To gain insight into the effect of arginine vasopressin (AVP) on renal hemodynamics in hypertensive rats, we investigated the vasoconstrictive response to AVP on total renal blood flow (RBF) and total and zonal glomerular filtration rate (GFR) in young and old spontaneously hypertensive rats (SHR). A hy...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000054225
更新日期:2001-01-01 00:00:00
abstract::The aim of this study was to investigate whether the renoprotective effect of angiotensin-converting enzyme inhibitors (ACEIs) following 5/6 renal mass reduction is due in part to the potentiation of kinins. Three groups of rats with 5/6 renal mass reduction were studied during the 14 weeks following surgery. One grou...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000025890
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND/AIMS:Ischemia/reperfusion (I/R) is the most common cause of acute renal injury. I/R-induced oxidative stress is involved in the development of acute renal injury, which can be reversed by supplementation with L-arginine, a precursor of nitric oxide (NO). This study was conducted to evaluate alterations in th...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000480362
更新日期:2017-01-01 00:00:00