Do mitochondria make nitric oxide? no?

Abstract:

:Several papers have claimed that mitochondria contain nitric oxide synthase (NOS) and make nitric oxide (NO*) in amounts sufficient to affect mitochondrial respiration. However, we found that the addition of L-arginine or the NOS inhibitor L-NMMA to intact rat liver mitochondria did not have any effect on the respiratory rate in both State 3 and State 4. We did not detect mitochondrial NO* production by the oxymyoglobin oxidation assay, or electrochemically using an NO* electrode. An apparent NO* production detected by the Griess assay was identified as an artifact. NO* generated by eNOS added to the mitochondria could easily be detected, although succinate-supplemented mitochondria appeared to consume NO*. Our data show that NO* production by normal rat liver mitochondria cannot be detected in our laboratory, even though the levels of production claimed in the literature should easily have been measured by the techniques used. The implications for the putative mitochondrial NOS are discussed.

journal_name

Free Radic Res

journal_title

Free radical research

authors

Tay YM,Lim KS,Sheu FS,Jenner A,Whiteman M,Wong KP,Halliwell B

doi

10.1080/10715760410001694008

keywords:

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

591-9

issue

6

eissn

1071-5762

issn

1029-2470

journal_volume

38

pub_type

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