Abstract:
:Several papers have claimed that mitochondria contain nitric oxide synthase (NOS) and make nitric oxide (NO*) in amounts sufficient to affect mitochondrial respiration. However, we found that the addition of L-arginine or the NOS inhibitor L-NMMA to intact rat liver mitochondria did not have any effect on the respiratory rate in both State 3 and State 4. We did not detect mitochondrial NO* production by the oxymyoglobin oxidation assay, or electrochemically using an NO* electrode. An apparent NO* production detected by the Griess assay was identified as an artifact. NO* generated by eNOS added to the mitochondria could easily be detected, although succinate-supplemented mitochondria appeared to consume NO*. Our data show that NO* production by normal rat liver mitochondria cannot be detected in our laboratory, even though the levels of production claimed in the literature should easily have been measured by the techniques used. The implications for the putative mitochondrial NOS are discussed.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Tay YM,Lim KS,Sheu FS,Jenner A,Whiteman M,Wong KP,Halliwell Bdoi
10.1080/10715760410001694008keywords:
subject
Has Abstractpub_date
2004-06-01 00:00:00pages
591-9issue
6eissn
1071-5762issn
1029-2470journal_volume
38pub_type
杂志文章abstract::It is well known that reactive oxygen species (ROS) attack several living tissues and increase the risk of development and progression of serious diseases. In neuronal level, ROS induce cell death in concentration-dependent fashion. However, little is known about the mechanisms of neuronal changes by ROS prior to indu...
journal_title:Free radical research
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journal_title:Free radical research
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journal_title:Free radical research
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journal_title:Free radical research
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journal_title:Free radical research
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journal_title:Free radical research
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abstract::In human organism, the gaseous radical molecule nitric oxide (NO) is produced in various cells from L-arginine by the catalytic action of NO synthases (NOS). The metabolic fate of NO includes oxidation to nitrate by oxyhaemoglobin in red blood cells and autoxidation in haemoglobin-free media to nitrite. Nitrate and ni...
journal_title:Free radical research
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更新日期:2005-08-01 00:00:00
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journal_title:Free radical research
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更新日期:2009-10-01 00:00:00
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journal_title:Free radical research
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doi:10.3109/10715762.2016.1152362
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journal_title:Free radical research
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doi:10.1080/10715762.2018.1524578
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pub_type: 杂志文章
doi:10.3109/10715769609149069
更新日期:1996-12-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001638010
更新日期:2004-05-01 00:00:00
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更新日期:2014-05-01 00:00:00
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journal_title:Free radical research
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