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Evidence for megakaryocyte engraftment following reduced-intensity conditioning.

Abstract：

:Assessment of donor chimerism is becoming increasingly important in patients undergoing reduced-intensity conditioning (RIC) allogeneic bone marrow transplants, due to the possibility of mixed chimeras. This regimen has been used successfully for patients with leukemia and genetic disorders with donor chimerism occurring in the myeloid, lymphoid, and/or erythroid lineages. Less toxic RIC expands the potential application of stem cell transplants to patients with nonmalignant disorders of hematopoiesis, such as the severe form of Glanzmann thrombasthenia, who previously were not considered suitable candidates based on risk-benefit analysis. To assess megakaryocyte/platelet chimerism after stem cell transplantation conducted with RIC, we used restriction fragment length polymorphism (RFLP) and sequence analyses of the HPA-3 polymorphism in the megakaryocyte/platelet-specific glycoprotein alphaIIb. In this study we show that at 23 weeks post-RIC, a leukemia patient acquired the HPA-3 donor phenotype at the DNA and platelet RNA levels.

journal_name

Exp Hematol

journal_title

Experimental hematology

authors

French DL,Fruchtman S,Mitchell WB,Coller BS,Peretz H

doi

10.1016/j.exphem.2004.05.028

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

877-80

issue

9

eissn

0301-472X

issn

1873-2399

pii

S0301-472X(04)00198-5

journal_volume

32

pub_type

杂志文章

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