Abstract:
:Previous studies suggested that nontypeable Haemophilus influenzae (NTHI) can form biofilms during human and chinchilla middle ear infections. Microscopic analysis of a 5-day biofilm of NTHI strain 2019 grown in a continuous-flow chamber revealed that the biofilm had a diffuse matrix interlaced with multiple water channels. Our studies showed that biofilm production was significantly decreased when a chemically defined medium lacking N-acetylneuraminic acid (sialic acid) was used. Based on these observations, we examined mutations in seven NTHI strain 2019 genes involved in carbohydrate and lipooligosaccharide biosynthesis. NTHI strain 2019 with mutations in the genes encoding CMP-N-acetylneuraminic acid synthetase (siaB), one of the three NTHI sialyltransferases (siaA), and the undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase homolog (wecA) produced significantly smaller amounts of biofilm. NTHI strain 2019 with mutations in genes encoding phosphoglucomutase (pgm), UDP-galactose-4-epimerase, and two other NTHI sialyltransferases (lic3A and lsgB) produced biofilms that were equivalent to or larger than the biofilms produced by the parent strain. The biofilm formed by the NTHI strain 2019pgm mutant was studied with Maackia amurensis fluorescein isothiocyanate (FITC)-conjugated and Sambucus nigra tetramethyl rhodamine isocyanate (TRITC)-conjugated lectins. S. nigra TRITC-conjugated lectin bound to this biofilm, while M. amurensis FITC-conjugated lectin did not. S. nigra TRITC-conjugated lectin binding was inhibited by incubation with alpha2,6-neuraminyllactose and by pretreatment of the biofilm with Vibrio cholerae neuraminidase. Matrix-assisted laser desorption ionization-time of flight mass spectometry analysis of lipooligosaccharides isolated from a biofilm, the planktonic phase, and plate-grown organisms showed that the levels of most sialylated glycoforms were two- to fourfold greater when the lipooligosaccharide was derived from planktonic or biofilm organisms. Our data indicate that NTHI strain 2019 produces a biofilm containing alpha2,6-linked sialic acid and that the sialic acid content of the lipooligosaccharides increases concomitant with the transition of organisms to a biofilm form.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Greiner LL,Watanabe H,Phillips NJ,Shao J,Morgan A,Zaleski A,Gibson BW,Apicella MAdoi
10.1128/IAI.72.7.4249-4260.2004keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
4249-60issue
7eissn
0019-9567issn
1098-5522pii
72/7/4249journal_volume
72pub_type
杂志文章abstract::The innate immune response to Pneumocystis infection is not well understood. In this study, normal C57BL/6 mouse alveolar macrophages were found to respond to Pneumocystis murina organisms through Toll-like receptor 2 (TLR2), leading to the nuclear translocation of NF-kappaB and the production of proinflammatory cytok...
journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7453-7460.2001
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journal_title:Infection and immunity
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doi:10.1128/IAI.32.1.111-117.1981
更新日期:1981-04-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.9.2.419-429.1974
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journal_title:Infection and immunity
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doi:10.1128/IAI.9.5.815-820.1974
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.56.12.3297-3300.1988
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01274-09
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journal_title:Infection and immunity
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doi:10.1128/IAI.25.1.208-212.1979
更新日期:1979-07-01 00:00:00
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doi:10.1128/IAI.00009-07
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1440-1443.2001
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2401-2403.1990
更新日期:1990-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4312-4318.2000
更新日期:2000-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5972-5979.1998
更新日期:1998-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.444-452.1977
更新日期:1977-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2655-2659.1998
更新日期:1998-06-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.27.3.808-816.1980
更新日期:1980-03-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.69.11.6776-6784.2001
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.3209-3212.1995
更新日期:1995-08-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.2.3.326-339.1970
更新日期:1970-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.3.489-496.1974
更新日期:1974-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.297-301.1980
更新日期:1980-02-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.00879-19
更新日期:2020-04-20 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.71-78.1982
更新日期:1982-01-01 00:00:00