Abstract:
:We evaluated the reproductive potential of frozen/thawed testicular spermatozoa of azoospermic men with left varicocele. The role of testicular tissue telomerase assay (TTA) in the prediction of the presence of testicular spermatozoa pre- and post-varicocelectomy was investigated, as well. Therapeutic testicular biopsy and TTA were performed in 82 nonobstructed azoospermic (NOA) men with varicoceles. Testicular spermatozoa were found in 33 men and processed for cryopreservation. Oocytes were later recovered from the spouses of the latter azoospermic men with varicoceles and injected with frozen/thawed testicular spermatozoa. Among the 49 men who were negative for testicular spermatozoa, 22 men underwent subsequently subinguinal microsurgical varicocelectomy. A total of 198 mature oocytes were successfully injected and 101 were normally fertilized and subsequently cleaved. Transfer of these 101 embryos in 26 women resulted in nine full-term pregnancies. Thirteen healthy babies were delivered. A cut-off value of TTA of 39 TPG U microg(-1) protein had an overall diagnostic accuracy equal to 90.2% to predict the presence of testicular spermatozoa pre-varicocelectomy. Within the group of men who were negative for testicular spermatozoa a cut-off value of TTA equal to 28 TPG U microg(-1) protein (pre-varicocelectomy) had a 84.2 % diagnostic accuracy to recognize the men who would become positive for either ejaculated or testicular spermatozoa post-varicocelectomy. Testicular spermatozoa can be found in 40% of NOA men with left varicocele. Ooplasmic injections with frozen/thawed testicular spermatozoa have a role in the therapeutic management of non-obstructive azoospermia associated with varicocele. Pre-varicocelectomy, a TTA cut-off value equal to 39 TPG U microg(-1) protein has a 90.2% diagnostic accuracy to indicate the men positive/negative for testicular spermatozoa. In addition, pre-varicocelectomy, a cut-off value equal to 28 TPG U microg(-1) protein has a 84.2% diagnostic accuracy to identify those men with varicoceles without testicular spermatozoa, who will become positive/negative for spermatozoa (either ejaculated or testicular) post-varicocelectomy.
journal_name
Andrologiajournal_title
Andrologiaauthors
Giannakis D,Baltogiannis D,Tsoukanelis K,Loutradis D,Miyagawa I,Makrydimas G,Kanakas N,Sofikitis Ndoi
10.1111/j.1439-0272.2004.00615.xkeywords:
subject
Has Abstractpub_date
2004-06-01 00:00:00pages
111-22issue
3eissn
0303-4569issn
1439-0272pii
AND615journal_volume
36pub_type
杂志文章相关文献
ANDROLOGIA文献大全abstract::The aim of this study was to find discriminatory parameters, based on sperm characteristics on the day of ovum pickup, that can help guide the decision to perform either intracytoplasmic sperm injection (ICSI) or in vitro fertilisation (IVF). We evaluated 112 cycles fertilised with both regular and ICSI insemination d...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2010.01107.x
更新日期:2012-04-01 00:00:00
abstract::The sperm function of fertile men (control), infertility patients (experimental), and men with varicocele were compared. The bioassays used were the follicular fluid-induced acrosome reaction, the binding to the zona pellucida, and the penetration of zona-free hamster oocytes. The percentage (mean +/- SEM) of reacted ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1994.tb00756.x
更新日期:1994-03-01 00:00:00
abstract::Nonorganic erectile dysfunction is a problem with unknown central mechanisms. Changes in brain activity in the amygdala have been observed in human patients. This study aimed to investigate the dopamine system in the basolateral amygdala of male rats with nonorganic erectile dysfunction. We applied chronic mild stress...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13160
更新日期:2019-02-01 00:00:00
abstract::The reference values of human semen, published in the WHO's latest edition in 2010, were lower than those previously reported. The objective of this study was to evaluate reference values of standard semen parameters in fertile Egyptian men. This cross-sectional study included 240 fertile men. Men were considered fert...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12942
更新日期:2018-01-15 00:00:00
abstract::The aim of this study was to investigate possible ultrasound seminal vesicle (SV) changes in infertile patients with 'hypertrophic-congestive' (HCUF) or 'fibro-sclerotic' (FSUF) ultrasound form of male accessory gland infection (MAGI) after prolonged administration of tadalafil (TAD), a selective phosphodiesterase-5 i...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12027
更新日期:2013-12-01 00:00:00
abstract::Previous study conducted in our department showed that 50% ethanolic extract of the root of Ricinus communis possess reversible antifertility effect and a 62-kDa protein (Rp) from this extract is responsible for the antifertility effects. In this study, we compared the spermicidal effect of this Rp with nonoxynol-9 (N...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2012.01291.x
更新日期:2012-12-01 00:00:00
abstract::Polyorchidism is defined as the presence of more than 2 histologically proven testes. We report the case of a 20-year-old man with polyorchidism, presenting with right scrotal pain and right scrotal ovoid mass. Scrotal examination revealed two ovoid, mobile lumps with testicular sensation in the right side of the scro...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0272.2011.01205.x
更新日期:2012-05-01 00:00:00
abstract::Emerging evidence is indicating the importance of hundreds of long noncoding RNAs (lncRNAs) in the occurrence and progression of different malignancies such as prostate cancer (PCa). This study investigated the possible role of HOX transcript antisense intergenic RNA myeloid-specific 1 (HOTAIRM1) in the progression of...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13897
更新日期:2020-12-23 00:00:00
abstract::A substantial body of evidence has accumulated in recent years that human semen quality may be deteriorating. This has been associated with evidence of other changes in male reproductive health, including increases in congenital malformations and testicular cancer in humans, and similar problems in wildlife. Unfortuna...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1046/j.1439-0272.2000.00388.x
更新日期:2000-09-01 00:00:00
abstract::The effects of incubation with caffeine at final concentration of 0.5 and 5.0 mM for 30 min at ambient temperature were examined in three normal semen samples and three from asthenozoospermic men. A 50 frames/s microcinematographic analysis of the sperm motility was performed and two characteristic parameters of the t...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1982.tb02295.x
更新日期:1982-09-01 00:00:00
abstract::Infertility occurs in up to 54% of men with bilateral undescended testes. Orchiectomy is considered the best therapeutic approach, especially when cryptorchidism is diagnosed in adulthood, due to a high risk of malignancy. A 33-year-old man was referred with a clinical presentation of empty scrotum and an ultrasonogra...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12936
更新日期:2018-03-01 00:00:00
abstract::A critical review of the gonadotropin therapy in male infertility or hypofertility is presented. In the author's opinion, the poor and conflicting results so far obtained wih this kind of treatment are mainly due to inadequate selection of the patients admitted to the treatment itself. The author stresses some points ...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0272.1981.tb00030.x
更新日期:1981-05-01 00:00:00
abstract::Previous studies have found that the ratio of estradiol to testosterone (E2/T ratio) has a negative effect on sexual function, but the relationship between the E2/T ratio and erection of the penis is not clarified. We conducted a retrospective study of 183 patients with erectile dysfunction and 52 healthy men to inves...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13701
更新日期:2020-10-01 00:00:00
abstract::In this study, a short stature male with infertility is reported. Semen analysis and serum concentrations of FSH, LH, T and PRL were estimated. Chromosome analysis was performed on lymphocytes obtained from both the male and his parents. Cytogenomic studies were performed by fluorescent in situ hybridisation and the C...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12278
更新日期:2015-05-01 00:00:00
abstract::The numbers of Sertoli and pre-meiotic germ cells in the developing rabbit testis were investigated as an initial step in determining the physiological meaning of the control of cell number in the testis by apoptosis. Sections were stained immunohistochemically for the detection of apoptotic cells and counterstained w...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1046/j.1439-0272.2002.00464.x
更新日期:2002-02-01 00:00:00
abstract::Human spermatozoa were incubated with gossypol acetic acid (100 micrograms/1 X 10(6) spermatozoa/ml) at 37 degrees C for 30 min. The drug treatment inhibited the spermatozoal motility significantly. Washing of the spermatozoa, after gossypol treatment, did not effect their motility. A partial revival in the motility o...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1986.tb01797.x
更新日期:1986-07-01 00:00:00
abstract::Tramadol, one of the most commonly abused drugs in Middle East, impacts spermatogenesis and disturbs reproductive hormones in animal studies. We aimed to investigate tramadol impact on sperm quality and on levels of testosterone, prolactin and gonadotropins, in tramadol abusers (n = 30) to age-matched control (n = 30)...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13026
更新日期:2018-08-01 00:00:00
abstract::The role of opioidergic system in controlling gonadotropin secretion in elderly men still remains uncertain. In the present study, we attempted to examine the opioidergic inhibitory tone imposed on GnRH release mechanism in elderly men by monitoring the plasma gonadotropin response to the opiate receptor antagonist na...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1994.tb00752.x
更新日期:1994-01-01 00:00:00
abstract::Corpus cavernosum smooth muscle cells (CCSMCs) are important functional cells for penile erection. We evaluated the effect of transforming growth factor β1 (TGFβ1) and hepatocyte growth factor (HGF) on the viability and apoptosis of CCSMCs in vitro. CCSMCs from healthy male Sprague Dawley rats were randomly divided in...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12372
更新日期:2015-11-01 00:00:00
abstract::Epididymal sperm reserve was determined in 6 sexually mature fertile buffalo-bulls having normal process of spermatogenesis. The relative distribution of spermatozoa in the caput, corpus and cauda epididymidis averaged 5.42, 0.75 and 11.45 billion respectively. Total epididymal sperm reserve per bull averaged 36.2 bil...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1978.tb03076.x
更新日期:1978-11-01 00:00:00
abstract::This short review summarizes some recent findings on arylhydrocarbon (dioxin) receptor expression during early pregnancy in mammals. The arylhydrocarbon receptor is a ligand-activated transcription factor and was originally described as a mediator of the toxic effects of dioxins and other chlorinated hydrocarbons such...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1046/j.1439-0272.2000.00397.x
更新日期:2000-09-01 00:00:00
abstract::Our laboratory has previously reported significantly low levels of protamine (PRM) gene expression in infertile men. This drop was correlated with a low pregnancy rate, suggesting that PRMs may be useful as predictive factors for the outcome of testicular sperm extraction and intracytoplasmic sperm injection (TESE-ICS...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2007.00775.x
更新日期:2007-08-01 00:00:00
abstract::Considering dopamine-enhancing effect of (+)-catechin, the present study was designed to evaluate dopamine-2 (D2) receptor agonistic and phosphodiesterase-5 (PDE5) enzyme inhibitory effects in in silico and effect on male sexual function of Sprague Dawley rats in vivo. (+)-Catechin and standard (sildenafil and bromocr...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13737
更新日期:2020-07-02 00:00:00
abstract::The cause of Sertoli cell-only syndrome (SCOS), a condition in which only Sertoli cells line the seminiferous tubules in the testis, is unknown. Three microarray data sets were downloaded from public databases and were used to compare SCOS and control group. A total of 291 genes differentially expressed (Log2 |FC| ≥ 1...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13288
更新日期:2019-08-01 00:00:00
abstract::The penile prosthesis has become established as a mode of therapy for organic and chronic psychogenic impotence. The Jonas type of penile prosthesis has been implanted into 10 patients and has achieved both functional success and excellent acceptance by the partner. Mechanical and pathological complications have a ver...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1984.tb00277.x
更新日期:1984-05-01 00:00:00
abstract::The objective of this study was to evaluate pubertal onset and characteristics in Egyptian boys. Examination of schoolboys (9-18 years) included height (cm), penile length (cm) and testicular volume (ml). Pubertal onset was considered at gonadal (G) stage 2 (G2 indicated testicular volume from 4-8 ml). Out of 1,078 bo...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13192
更新日期:2019-03-01 00:00:00
abstract::A careful history and evaluation of men with chronic orchialgia elucidates the aetiology in some men to be a hyperactive cremaster muscle reflex with testicular retraction as the cause. The objective is to evaluate outcomes in men who underwent microsurgical subinguinal cremaster muscle release (MSCMR) with a retrospe...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13493
更新日期:2020-02-01 00:00:00
abstract::Partial priapism is a rare disorder in literature and generally described as a contusion or thrombosis of the cavernous body of the penis secondary to blunt trauma. Because of the rarity of disease, there is not much information about the treatment. Conservative management with nonsteroidal anti-inflammatory drug is o...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13510
更新日期:2020-04-01 00:00:00
abstract::The aim of this study was to comprise the effect of catalase on sperm parameters and chromatin in normospermic persons. Semen samples were obtained from fertile men. A certain amount of different concentrations of catalase (0.1, 1, 10, 50, 100, 150 and 200 IU.ml) was added to each vial containing semen. Control group ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13231
更新日期:2019-05-01 00:00:00
abstract::Monoclonal and polyclonal antibodies were used in indirect immunofluorescence microscopy to localize vimentin intermediate filaments in the rat seminiferous epithelium. During stages XII-V of the epithelial cycle, the Sertoli cells showed a reaction in the perinuclear area and vimentin-positive extensions, projecting ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1987.tb01920.x
更新日期:1987-11-01 00:00:00