当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF PHARMACEUTICS期刊下所有文献 > Comparative evaluation of cytotoxicity of a glucosamine-TBA conjugate and a chitosan-TBA conjugate.

Comparative evaluation of cytotoxicity of a glucosamine-TBA conjugate and a chitosan-TBA conjugate.

Abstract：

:D-glucosamine and chitosan were modified by the immobilization of thiol groups utilizing 2-iminothiolane. The toxicity profile of the resulting D-glucosamine-TBA (4-thiobutylamidine) conjugate, of chitosan-TBA conjugate and of the corresponding unmodified controls was evaluated in vitro. On the one hand, the cell membrane damaging effect of 0.025% solutions of the test compounds was investigated via red blood cell lysis test. On the other hand, the cytotoxity of 0.025, 0.25 and 0.5% solutions of the test compounds was evaluated on L-929 mouse fibroblast cells utilizing two different bioassays: the MTT assay (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide), which assess the mitochondrial metabolic activity of the cells, and the BrdU-based enzyme-linked immunosorbent assay, which measures the incorporation in the DNA of 5-bromo-2'-deoxyuridine and consequently the cell proliferation. Results of the red blood cell lysis test showed that both thiolated compounds displayed a lower membrane damaging effect causing a significantly lower haemoglobine release than the unmodified compounds. Data obtained by the MTT assay and the BrdU assay revealed a concentration dependent relative cytotoxicity for all tested compounds. The covalent linkage of the TBA-substructure to D-glucosamine did not cause a significant increase in cytotoxicity, whereas at higher concentrations a slightly enhanced cytotoxic effect was caused by the derivatisation of chitosan. In conclusion, the -TBA derivatives show a comparable toxicity profile to the corresponding unmodified compounds, which should not compromise their future use as save pharmaceutical excipients.

journal_name

Int J Pharm

journal_title

International journal of pharmaceutics

authors

Guggi D,Langoth N,Hoffer MH,Wirth M,Bernkop-Schnürch A

doi

10.1016/j.ijpharm.2004.03.016

keywords:

subject

Has Abstract

pub_date

2004-07-08 00:00:00

pages

353-60

issue

2

eissn

0378-5173

issn

1873-3476

pii

S0378517304002030

journal_volume

278

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF PHARMACEUTICS文献大全
  • Preparation of surface modified protein nanoparticles by introduction of sulfhydryl groups.

    abstract：:The objective of the present study was to establish several methods for the introduction of thiol groups onto the surface of human serum albumin (HSA) nanoparticles. Besides the epsilon-amino groups of lysine, the carboxyl groups of asparaginic and glutaminic acid, and the carbonyl groups of the cross-linker glutarald...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(00)00590-1

    authors： Weber C,Reiss S,Langer K

    更新日期：2000-12-15 00:00:00

  • Ciprofloxacin surf-plexes in sub-micron emulsions: a novel approach to improve payload efficiency and antimicrobial efficacy.

    abstract：:The aim of this study was to investigate antimicrobial efficacy and pharmacokinetic profile of ciprofloxacin (CFn) loaded oil-in-water (o/w) submicron emulsion (SE-CFn). This study emphasized on development of hydrophobic ion-pair complexes of CFn with sodium deoxycholate (SDC) [CFn-SDC], which was incorporated in the...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.02.020

    authors： Jain V,Singodia D,Gupta GK,Garg D,Keshava GB,Shukla R,Shukla PK,Mishra PR

    更新日期：2011-05-16 00:00:00

  • Carbosilane dendrimers with phosphonium terminal groups are low toxic non-viral transfection vectors for siRNA cell delivery.

    abstract：:Non-viral gene delivery vectors studied in the gene therapy applications are often designed with the cationic nitrogen containing groups necessary for binding and cell release of nucleic acids. Disadvantage is a relatively high toxicity which restricts the in vivo use of such nanoparticles. Here we show, that the 3rd ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.03.018

    authors： Herma R,Wrobel D,Liegertová M,Müllerová M,Strašák T,Maly M,Semerádtová A,Štofik M,Appelhans D,Maly J

    更新日期：2019-05-01 00:00:00

  • Synthesis and evaluation of styrene-maleic acid copolymer conjugated amphotericin B.

    abstract：:Amphotericin B (AmB), which plays a central role in the treatment of systemic fungal infections, is difficult to formulate because it's sparingly soluble in water and organic solvents. We previously prepared AmB-loaded micelles using styrene-maleic acid copolymer (SMA). Although solubilization was achieved by this for...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.118719

    authors： Banshoya K,Kaneo Y,Tanaka T,Yamamoto S,Maeda H

    更新日期：2019-12-15 00:00:00

  • Heparin-functionalized chitosan-alginate scaffolds for controlled release of growth factor.

    abstract：:Controlled long-term release of basic fibroblast growth factor (bFGF) has shown a combined effect on the stimulation of regenerating a number of tissues including cartilage, nerve, skin and liver. In this study, three-dimensional scaffolds prepared from the polyelectrolyte complexes (PEC) of chitosan and alginate were...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.04.048

    authors： Ho YC,Mi FL,Sung HW,Kuo PL

    更新日期：2009-07-06 00:00:00

  • Stabilization of gene delivery systems by freeze-drying.

    abstract：:Freeze-drying of three different forms of gene delivery systems was performed using a controlled two-step drying process and 10% sucrose as lyoprotectant. Complexes of pCMVL plasmid with transferrin-conjugated polyethylenimine, adenovirus-enhanced transferrinfection consisting of pCMVL/transferrin-polylysine complexes...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(97)00244-5

    authors： Talsma H,Cherng J,Lehrmann H,Kursa M,Ogris M,Hennink WE,Cotten M,Wagner E

    更新日期：1997-11-28 00:00:00

  • Controlled dual release of basic fibroblast growth factor and indomethacin from heparin-conjugated polymeric micelle.

    abstract：:This work describes the development of heparinized polymeric micelle as a novel injectable carrier for the dual drug delivery that can simultaneously release basic fibroblast growth factor (bFGF) and indomethacin (IMC), which can promote the regeneration of damaged tissue and prevent the inflammatory response after im...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2007.06.025

    authors： Lee JS,Bae JW,Joung YK,Lee SJ,Han DK,Park KD

    更新日期：2008-01-04 00:00:00

  • Retention and distribution of two 99mTc-DTPA labelled vaginal dosage forms.

    abstract：UNLABELLED:To objectively evaluate the performance of new vaginal dosage forms, it is important to determine their time of residence and their distribution. This paper describes the in vivo characteristics of a reference and test product in this situation. METHOD:A randomised cross-over study was performed in the same...

    journal_title：International journal of pharmaceutics

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/j.ijpharm.2003.11.006

    authors： Chatterton BE,Penglis S,Kovacs JC,Presnell B,Hunt B

    更新日期：2004-03-01 00:00:00

  • Surfactants in membrane solubilisation.

    abstract：:An understanding of the action of many drugs requires a knowledge of how the drug reaches the site of action in a cell. A detailed knowledge of the structure and function of cell membranes is often required to understand the transport of drugs across the plasma membrane. To obtain this information proteins must be iso...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章,评审

    doi：10.1016/s0378-5173(98)00345-7

    authors： Jones MN

    更新日期：1999-01-25 00:00:00

  • Effect of process parameters on compressibility of granulation manufactured in a high-shear mixer.

    abstract：:Various processing variables that can influence granulation characteristics of a lactose-based formulation were evaluated using a Plackett-Burman experimental design. These parameters were impeller speed, granulating solution addition rate, total amount of water added in the granulation step, wet massing time, moistur...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(99)00445-7

    authors： Badawy SI,Menning MM,Gorko MA,Gilbert DL

    更新日期：2000-03-30 00:00:00

  • In vitro skin penetration enhancement techniques: A combined approach of ethosomes and microneedles.

    abstract：:Dermal administration of different macromolecules, such as nucleic acids, remains a real challenge because of the difficulty of crossing the main skin barrier, the stratum corneum (SC). To overcome this barrier, the use of deformable lipid-based nanovectors were developed to increase topical penetration through the SC...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.118793

    authors： Bellefroid C,Lechanteur A,Evrard B,Mottet D,Debacq-Chainiaux F,Piel G

    更新日期：2019-12-15 00:00:00

  • The effect of polyoxyethylene polymers on the transport of ranitidine in Caco-2 cell monolayers.

    abstract：:Previous in vivo studies using PEG 400 showed an enhancement in the bioavailability of ranitidine. This study investigated the effect of PEG 200, 300 and 400 on ranitidine transport across Caco-2 cells. The effect of PEG polymers (20%, v/v) on the bi-directional flux of (3)H-ranitidine across Caco-2 cell monolayers wa...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.02.059

    authors： Ashiru-Oredope DA,Patel N,Forbes B,Patel R,Basit AW

    更新日期：2011-05-16 00:00:00

  • Nanoemulsion enhances α-tocopherol succinate bioavailability in rats.

    abstract：:The vitamin E analogue, α-tocopherol succinate (α-TOS), has a broad anti-tumor effect. α-TOS can induce cancer cells apoptosis and suppress tumor growth by targeting mitochondria. Low bioavailability of α-TOS is the major problem encountered with formulation development. In our study, α-TOS nanoemulsion (α-TOS-NE) was...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2016.10.026

    authors： Gao Y,Qi X,Zheng Y,Ji H,Wu L,Zheng N,Tang J

    更新日期：2016-12-30 00:00:00

  • The dispersion behaviour of dry powder inhalation formulations cannot be assessed at a single inhalation flow rate.

    abstract：:The dispersion performances of inhalation powders are often tested at only one inhalation flow rate in mechanistic formulation studies. This limited approach is challenged by studies showing that interactions exist between inhalation flow rate and the effects on dispersion performance of several formulation variables....

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2014.02.024

    authors： Grasmeijer F,de Boer AH

    更新日期：2014-04-25 00:00:00

  • Investigation of the phase behaviour of systems containing lecithin and 2-acyl lysolecithin derivatives.

    abstract：:A series of modified phospholipids (m-PC) possessing different acyl chains in position 2, from butanoyl to hexadecanoyl, were prepared by partial synthesis from soybean lysolecithin. They were used with soybean lecithin to construct phase diagrams containing ethanol as cosolvent, water and medium chain triglycerides (...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(99)00281-1

    authors： Trotta M,Gallarate M,Pattarino F,Carlotti ME

    更新日期：1999-11-10 00:00:00

  • Porous clay heterostructures: A new inorganic host for 5-fluorouracil encapsulation.

    abstract：:This study proposed a new inorganic host for drug encapsulation. Porous clay heterostructure (PCH), synthesized using modified montmorillonite with hexadecyltrimethylammonium bromide, was used as host material and 5-fluorouracil (5-FU) as guest drug. Drug encapsulation within PCH in different conditions (soaking time,...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2015.05.053

    authors： Gârea SA,Mihai AI,Ghebaur A,Nistor C,Sârbu A

    更新日期：2015-08-01 00:00:00

  • Glucan particles as suitable carriers for the natural anti-inflammatory compounds curcumin and diplacone - Evaluation in an ex vivo model.

    abstract：:Natural compounds offer a wide spectrum of potential active substances, but often they have a poor bioavailability. To increase the bioavailability and bioactivity of the natural anti-inflammatory molecules curcumin and diplacone, we used glucan particles (GPs), hollow shells from Saccharomyces cerevisiae composed mai...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2020.119318

    authors： Rotrekl D,Devriendt B,Cox E,Kavanová L,Faldyna M,Šalamúnová P,Baďo Z,Prokopec V,Štěpánek F,Hanuš J,Hošek J

    更新日期：2020-05-30 00:00:00

  • Comparative study of Pluronic(®) F127-modified liposomes and chitosan-modified liposomes for mucus penetration and oral absorption of cyclosporine A in rats.

    abstract：:Liposomes modified using cationic and hydrophilic nonionic polymers are 2 popular carriers for improving oral drug absorption. Cationic polymer-modified liposomes can adhere to the intestinal wall mucus (mucoadhesive type), while liposomes modified using hydrophilic nonionic polymers can penetrate across the mucus bar...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2013.04.002

    authors： Chen D,Xia D,Li X,Zhu Q,Yu H,Zhu C,Gan Y

    更新日期：2013-06-05 00:00:00

  • Occlusion effect on transcutaneous NSAID delivery from conventional and carrier-based formulations.

    abstract：:We studied skin occlusion effects in vitro and in vivo on local and systemic delivery of ketoprofen across the organ, using the drug in a conventional non-occlusive topical gel (Togal Mobil-Gel), an occlusive tape (Mohrus), and the new targeted analgesic (Diractin), comprising ultradeformable, hydrophilic carriers in ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2008.04.005

    authors： Cevc G,Mazgareanu S,Rother M,Vierl U

    更新日期：2008-07-09 00:00:00

  • Preparation and characterization of stable nanoliposomal formulations of curcumin with high loading efficacy: In vitro and in vivo anti-tumor study.

    abstract：:Liposomal formulations were made using Solvent-assisted active loading technology (SALT). Two formulations composed of HSPC:DPPG:Chol:DSPE-mPEG2000 (PG-LipCUR) and HSPC:Chol:DSPE-mPEG2000 (LipCUR) demonstrated good colloidal properties and the CUR-encapsulation of 82% and 89% for PG-LipCUR and LipCUR, respectively. Th...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2020.119211

    authors： Karimi M,Gheybi F,Zamani P,Mashreghi M,Golmohammadzadeh S,Darban SA,Badiee A,Jaafari MR

    更新日期：2020-04-30 00:00:00

  • Critical evaluation of cadexomer-iodine ointment and povidone-iodine sugar ointment.

    abstract：:Topical iodine forms are used for infected and necrotic pressure ulcers. Despite antimicrobial advantages several potential disadvantages were observed with controversial results. To clarify the controversy, the reactivity of povidone-iodine (PI) sugar ointment and cadexomer-iodine (CI) ointment toward biological comp...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.01.007

    authors： Noda Y,Fujii K,Fujii S

    更新日期：2009-05-08 00:00:00

  • Achieving delayed release of freeze-dried probiotic strains by extrusion, spheronization and fluid bed coating - evaluated using a three-step in vitro model.

    abstract：:Intake of probiotics is associated with many health benefits, which has generated an interest in formulating viable probiotic supplements. The present study had two aims. The first aim was to achieve gastrointestinal protection and delayed release of viable probiotics by pelletizing and coating freeze-dried probiotic ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2020.120022

    authors： Jacobsen NMY,Caglayan I,Caglayan A,Bar-Shalom D,Müllertz A

    更新日期：2020-12-15 00:00:00

  • Correlation of laboratory and production freeze drying cycles.

    abstract：:The purpose of this study was to develop the correlation of cycle parameters between a laboratory and a production freeze-dryer. With the established correlation, key cycle parameters obtained using a laboratory dryer may be converted to those for a production dryer with minimal experimental efforts. In order to devel...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2005.06.022

    authors： Kuu WY,Hardwick LM,Akers MJ

    更新日期：2005-09-30 00:00:00

  • Permeation of several drugs through keratinized epithelial-free membrane of hamster cheek pouch.

    abstract：:The hamster cheek pouch mucosa was selected as a substitute for the human buccal mucosa in an in vitro permeation study. Considering that a keratinized layer is not present in the human buccal mucosa, keratinized epithelial-free hamster cheek pouch (KEF-membrane) was prepared by chemical splitting. To confirm the usef...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(98)00329-9

    authors： Tsutsumi K,Obata Y,Takayama K,Isowa K,Nagai T

    更新日期：1999-01-15 00:00:00

  • A comparative study of wound dressings loaded with silver sulfadiazine and silver nanoparticles: In vitro and in vivo evaluation.

    abstract：:In the current study, two series of antimicrobial dressings conjugated with silver sulfadiazine (SSD) and silver nanoparticles (AgNPs) were developed and evaluated for chronic wound healing. Highly porous polycaprolactone (PCL)/polyvinyl alcohol (PVA) nanofibers were loaded with different concentrations of SSD or AgNP...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.04.068

    authors： Mohseni M,Shamloo A,Aghababaie Z,Afjoul H,Abdi S,Moravvej H,Vossoughi M

    更新日期：2019-06-10 00:00:00

  • Development of cyclosporine A-loaded dry-emulsion formulation using highly purified glycerol monooleate for safe inhalation therapy.

    abstract：:The main objective of this study was to improve the safety and oxidative stability of glycerol monooleate (GMO)-based dry-emulsion (DE) formulation containing cyclosporine A (CsA) for inhalation therapy. GMO or highly purified GMO (hpGMO) was used as surfactant for the DE formulations (GMO/DE or hpGMO/DE), the toxicol...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2013.03.026

    authors： Sato H,Ogawa K,Kojo Y,Kawabata Y,Mizumoto T,Yamada S,Onoue S

    更新日期：2013-05-01 00:00:00

  • Understanding die fill variation during mini-tablet production.

    abstract：:Reproducibility of die fill during tablet production is critical to ensure consistent tablet drug content and mechanical attributes. In the production of mini-tablets, tablets smaller than 6mm, achievement of uniform die fill is much more challenging. Powder flow is often associated with die fill accuracy but this rel...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.10.042

    authors： Goh HP,Heng PWS,Liew CV

    更新日期：2017-12-20 00:00:00

  • Evaluation of sorption capacity of antibiotics and antibacterial properties of a cyclodextrin-polymer functionalized hydroxyapatite-coated titanium hip prosthesis.

    abstract：:Infection still present as one of common complications after total hip replacement (∼2.5%), which may cause serious outcomes. For preventing such risk, loading antibiotics onto implants for increasing local drug concentration at targeted sites could be a solution. This study aims at modifying the surface of hydroxyapa...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2014.10.026

    authors： Taha M,Chai F,Blanchemain N,Neut C,Goube M,Maton M,Martel B,Hildebrand HF

    更新日期：2014-12-30 00:00:00

  • An application of deep learning to detect process upset during pharmaceutical manufacturing using passive acoustic emissions.

    abstract：:The multivariate nature of a fluidized bed system creates process complexity that increases the risk of production upset. This research explores the use of passive acoustic emissions monitoring paired with an artificial neural network to detect fluidized bed distributor plate blockage. In many cases, early process fai...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2018.08.052

    authors： Carter A,Briens L

    更新日期：2018-12-01 00:00:00

  • Preparation of poly(DL-lactide-co-glycolide) nanoparticles by modified spontaneous emulsification solvent diffusion method.

    abstract：PURPOSE:The objectives of this study were to establish a new preparation method for poly(DL-lactide-co-glycolide) (PLGA) nanoparticles by modifying the spontaneous emulsification solvent diffusion (SESD) method and to elucidate the mechanism of nanoparticle formation on the basis of the phase separation principle of PL...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(99)00187-8

    authors： Murakami H,Kobayashi M,Takeuchi H,Kawashima Y

    更新日期：1999-10-05 00:00:00