GSTA is a major glutathione S-transferase gene responsible for 4-hydroxynonenal conjugation in largemouth bass liver.

Abstract:

:We have previously shown that largemouth bass (Micropterus salmoides) has a remarkable ability to conjugate 4-hydroxy-2-nonenal (4HNE), a mutagenic and cytotoxic alpha,beta-unsaturated aldehyde produced during the peroxidation of lipids. In addition, we have isolated a glutathione S-transferase cDNA (bass GSTA) that encodes a recombinant protein which is highly active in 4HNE conjugation and structurally similar to plaice (Pleuronectes platessa) GSTA. In the present study, HPLC-GST subunit analysis revealed the presence of at least two major GST isoforms in bass liver, with one peak constituting 80% of the total bass liver GST protein. Liquid chromatography mass spectrometry (LC-MS) and electrospray ionization analysis of the major bass GST subunit yielded a molecular weight of 26,396 kDa. Endo-proteinase Lys-C digestion and Edman degradation protein sequencing of this GST peak demonstrated that this protein was encoded by bass GSTA. Analysis of genomic DNA fragments isolated by nested PCR indicated the presence of a GST gene cluster in bass liver that contained GSTA, and was similar to a GST gene cluster characterized by Leaver et al., in plaice. Collectively, our data indicates the presence of a major GST in bass liver involved in the protection against oxidative stress. This GST is part of a gene cluster that may be conserved in certain freshwater and marine fish.

journal_name

Mar Environ Res

authors

Pham RT,Barber DS,Gallagher EP

doi

10.1016/j.marenvres.2004.03.033

keywords:

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

485-8

issue

2-5

eissn

0141-1136

issn

1879-0291

pii

S0141-1136(04)00084-4

journal_volume

58

pub_type

杂志文章
  • Contrasting effects of constant and fluctuating pCO2 conditions on the exercise physiology of coral reef fishes.

    abstract::Ocean acidification (OA) is predicted to affect the physiology of some fishes. To date, most studies have investigated this issue using stable pCO2 levels based on open ocean projections. Yet, most shallow, nearshore systems experience temporal and spatial pCO2 fluctuations. For example, pCO2 on coral reefs is highest...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2020.105224

    authors: Hannan KD,McMahon SJ,Munday PL,Rummer JL

    更新日期:2021-01-01 00:00:00

  • The behaviors of microplastics in the marine environment.

    abstract::Despite the pollution of microplastics being internationally recognized, the understanding of their behaviors in marine environment is still developing. Microplastics are ubiquitous in the marine environment, with the potential to cause harm to marine ecosystem. Here, we would classify the behaviors of microplastics a...

    journal_title:Marine environmental research

    pub_type: 杂志文章,评审

    doi:10.1016/j.marenvres.2015.10.014

    authors: Wang J,Tan Z,Peng J,Qiu Q,Li M

    更新日期:2016-02-01 00:00:00

  • Lindane toxicity: histopathological, behavioural and biochemical changes in Etroplus maculatus (Bloch, 1795).

    abstract::The effects of sub-lethal concentrations of lindane on behavioral, hematological and histological parameters in Etroplus maculatus were investigated. The LC₅₀ value of lindane for E. maculatus was estimated using the Probit method and was found to be 0.028 mg/L. The red blood cell count (RBC) and the hemoglobin (Hb), ...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2011.10.011

    authors: Bijoy Nandan S,Nimila PJ

    更新日期:2012-05-01 00:00:00

  • Assessment of putative endocrine disrupters in an in vivo crustacean assay and an in vitro insect assay.

    abstract::Concern over endocrine disrupters in coastal ecosystems has stimulated global efforts to understand their potential impacts on fish and invertebrate communities. Given that marine crustaceans are sensitive to the effects of alkylphenols and other xenobiotics, we are currently investigating whether these responses may ...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/s0141-1136(02)00113-7

    authors: Pounds NA,Hutchinson TH,Williams TD,Whiting P,Dinan L

    更新日期:2002-09-01 00:00:00

  • Recruitment of benthic organisms onto a planned artificial reef: shifts in community structure one decade post-deployment.

    abstract::Most artificial reef (AR) studies have examined the early colonization stages of benthic communities, while only a few have monitored the development of AR communities beyond the initial successional phases and evaluated the time scale needed for such development. In addition, despite the proliferation of AR studies, ...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2004.03.122

    authors: Perkol-Finkel S,Benayahu Y

    更新日期:2005-03-01 00:00:00

  • Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene.

    abstract::Juvenile turbot (Scophthalmus maximus) were injected intraperitoneally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled I and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydro...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/s0141-1136(02)00192-7

    authors: Telli-Karakoç F,Ruddock PJ,Bird DJ,Hewer A,Van Schanke A,Phillips DH,Peters LD

    更新日期:2002-09-01 00:00:00

  • Trophic ecology of the sea urchin Spatangus purpureus elucidated from gonad fatty acids composition analysis.

    abstract::Irregular sea urchins such as the spatangoid Spatangus purpureus are important bioturbators that contribute to natural biogenic disturbance and the functioning of biogeochemical cycles in soft sediments. In the coastal waters of the Balearic Islands S. purpureus occurs in soft red algal beds, and can reach high densit...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2011.01.008

    authors: Barberá C,Fernández-Jover D,López Jiménez JA,González Silvera D,Hinz H,Moranta J

    更新日期:2011-05-01 00:00:00

  • Effects of natural current pH variability on the sea urchin Paracentrotus lividus larvae development and settlement.

    abstract::One of the most important environmental factors controlling the distribution, physiology, morphology and behaviour of marine invertebrates is ocean pH. In the last decade, the effects of decreasing ocean pH as a result of climate change processes (i.e. ocean acidification) on marine organisms have been target of much ...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2018.04.012

    authors: García E,Clemente S,Hernández JC

    更新日期:2018-08-01 00:00:00

  • Evaluation of microplastic toxicity in accordance with different sizes and exposure times in the marine copepod Tigriopus japonicus.

    abstract::The indiscriminate use of plastic has greatly increased microplastic contamination risk in the marine environment. Microplastics can affect all marine life via the food web, from primary producers (e.g., microalgae) to final consumers (e.g., carnivorous fish). Thus, several studies have attempted to evaluate microplas...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2019.104838

    authors: Choi JS,Hong SH,Park JW

    更新日期:2019-11-09 00:00:00

  • Applying an integrated approach to coastal marine habitat mapping in the north-western United Arab Emirates.

    abstract::Habitat mapping is essential for the management and conservation of coastal marine habitats. However, accurate and up-to-date habitat maps are rarely available for the marine realm. In this study, we mapped the coastal marine habitats of >400 km of coastline in the north-western United Arab Emirates (UAE) using a comb...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2020.105095

    authors: Mateos-Molina D,Antonopoulou M,Baldwin R,Bejarano I,Burt JA,García-Charton JA,Al-Ghais SM,Walgamage J,Taylor OJS

    更新日期:2020-10-01 00:00:00

  • Oxidative stress in Laeonereis acuta (Polychaeta, Nereididae): environmental and seasonal effects.

    abstract::Laeonereis acuta was seasonally collected in an industrially polluted site (P) and in an unpolluted site (UP) at the Patos Lagoon estuary (southern Brazil). Glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activity (U/mg protein) was determined in five groups of worms from each site. Meta...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2004.03.053

    authors: Geracitano LA,Monserrat JM,Bianchini A

    更新日期:2004-08-01 00:00:00

  • The expression pattern of hsp70 plays a critical role in thermal tolerance of marine demersal fish: Multilevel responses of Paralichthys olivaceus and its hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic and acute heat stress.

    abstract::Ocean warming has multifaceted impacts on marine organisms. This study investigated the different responses of Paralichthys olivaceus and the hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic and acute heat stress. By comparing their survival, behavioural and histological changes, we found that the hybrids possess a...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2017.06.015

    authors: Liu Y,Ma D,Zhao C,Xiao Z,Xu S,Xiao Y,Wang Y,Liu Q,Li J

    更新日期:2017-08-01 00:00:00

  • An assessment of microplastics threat to the marine environment: A short review in context of the Arabian/Persian Gulf.

    abstract::Microplastics are recognised as a (persistent) pollutant and are believed to be ubiquitous in the marine environment. The importance of this issue is evident from the large number of technical publications and research efforts within the past decade. However, the Arabian (Persian) Gulf region has few reported datasets...

    journal_title:Marine environmental research

    pub_type: 杂志文章,评审

    doi:10.1016/j.marenvres.2020.104961

    authors: Al-Salem SM,Uddin S,Al-Yamani F

    更新日期:2020-07-01 00:00:00

  • Ecotoxicological effects of polystyrene microbeads in a battery of marine organisms belonging to different trophic levels.

    abstract::The aim of this study was to detect ecotoxicological effects of 0.1 μm polystyrene microbeads in marine organisms belonging to different trophic levels. MP build up, lethal and sub-lethal responses were investigated in the bacterium Vibrio anguillarum (culturability), in the green microalga Dunaliella tertiolecta (gro...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2018.09.023

    authors: Gambardella C,Morgana S,Bramini M,Rotini A,Manfra L,Migliore L,Piazza V,Garaventa F,Faimali M

    更新日期:2018-10-01 00:00:00

  • Tide- and rainfall-induced variations of physical and chemical parameters in a mangrove-depleted estuary of East Hainan (South China Sea).

    abstract::The estuarine dynamics favoring the coexistence of mangroves, seagrass and corals at small river mouths are often poorly understood. We characterize the tidal, day/night and rainfall-induced short-term dynamics in salinity, pH, dissolved oxygen (DO), chlorophyll a (chl a), total suspended matter (TSM), water transpare...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2012.09.002

    authors: Krumme U,Herbeck LS,Wang T

    更新日期:2012-12-01 00:00:00

  • Bacterial community dynamic associated with autochthonous bioaugmentation for enhanced Cu phytoremediation of salt-marsh sediments.

    abstract::Autochthonous bioaugmentation for metal phytoremediation is still little explored, particularly its application to estuarine salt marshes, but results obtained so far are promising. Nevertheless, understanding the behaviour of the microbial communities in the process of bioaugmentation and their role in improving meta...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2017.10.007

    authors: Almeida CMR,Oliveira T,Reis I,Gomes CR,Mucha AP

    更新日期:2017-12-01 00:00:00

  • Measurement of the activity of multixenobiotic resistance mechanism in the common carp Cyprinus carpio.

    abstract::The multixenobiotic resistance (MXR) mechanism, mediated by activity of the transmembrane P-glycoprotein, represents a basic biological defence system in aquatic organisms. Here we investigate the MXR transport activity in an aquatic vertebrate, the common carp (Cyprinus carpio). We measured the accumulation rate of a...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/s0141-1136(02)00155-1

    authors: Smital T,Sauerborn R

    更新日期:2002-09-01 00:00:00

  • cDNA cloning, sequencing, and differential expression of a heart-type fatty acid-binding protein in the mummichog (Fundulus heteroclitus).

    abstract::A heart fatty acid-binding protein (H-FABP) cDNA was isolated from the liver of the mummichog (Fundulus heteroclitus) and the complete cDNA sequence and predicted amino acid sequence was determined. This cDNA binds to a mRNA product of 0.95 kb with the highest level of RNA expression in the male liver, gills, and gona...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/s0141-1136(02)00130-7

    authors: Bain LJ

    更新日期:2002-09-01 00:00:00

  • Vertical distribution of lipids, fatty acids and organochlorine contaminants in the blubber of southern hemisphere humpback whales (Megaptera novaeangliae).

    abstract::Persistent organic pollutants (POPs), such as toxic lipophilic organochlorine (OC) compounds, accumulate in the blubber tissue of marine mammals. Toxicological sampling methods most frequently target only the superficial blubber layer. Vertical distribution of these contaminants through the blubber mantle may, however...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2013.11.004

    authors: Waugh CA,Nichols PD,Schlabach M,Noad M,Bengtson Nash S

    更新日期:2014-03-01 00:00:00

  • Development of hepatic CYP1A and blood vitellogenin in eel (Anguilla anguilla) for use as biomarkers in the Thames Estuary, UK.

    abstract::The potential of eel (Anguilla anguilla) as a monitoring species for the Thames Estuary, UK, was examined. Hepatic cytochrome P4501A [7-ethoxyresorufin O-deethylase (EROD) activity] and blood vitellogenin (Western analysis) were investigated as biomarkers of exposure to, respectively, organic contaminants and to conta...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/s0141-1136(00)00060-x

    authors: Livingstone DR,Mitchelmore CL,Peters LD,O'Hara SC,Shaw JP,Chesman BS,Doyotte A,McEvoy J,Ronisz D,Larsson DG,Förlin L

    更新日期:2000-07-01 00:00:00

  • Bioaccumulation of PCB congeners in marine benthic infauna.

    abstract::Field concentrations of polychlorinated biphenyls (PCBs) were measured in sediment and nine marine soft bottom invertebrate species. Lipid- and organic carbon normalised biota-sediment accumulation factors (BSAFs) were determined for 29 nonplanar and 11 coplanar congeners. To investigate whether the bioaccumulation wa...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2005.11.004

    authors: Magnusson K,Ekelund R,Grabic R,Bergqvist PA

    更新日期:2006-05-01 00:00:00

  • A multibiomarker approach in the clam Ruditapes decussatus to assess the impact of pollution in the Ria Formosa lagoon, South Coast of Portugal.

    abstract::The Ria Formosa lagoon is an ecosystem whose water quality reflects the anthropogenic influence upon the surrounding areas. In this lagoon, the clam Ruditapes decussatus has a great economical importance and has been widely used as a biomonitor. A multibiomarker approach (δ-aminolevulinic acid dehydratase, metallothio...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2011.09.012

    authors: Cravo A,Pereira C,Gomes T,Cardoso C,Serafim A,Almeida C,Rocha T,Lopes B,Company R,Medeiros A,Norberto R,Pereira R,Araújo O,Bebianno MJ

    更新日期:2012-04-01 00:00:00

  • Estimating intertidal seaweed biomass at larger scales from quadrat surveys.

    abstract::The amount of macroalgal biomass is an important ecosystem variable. Estimates can be made for a sampled area or values can be extrapolated to represent biomass over a larger region. Typically biomass is scaled-up using the area multiplied by the mean: a non-spatial method. Where algal biomass is patchy or shows gradi...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2020.104906

    authors: Johnson MP

    更新日期:2020-04-01 00:00:00

  • Environmental heterogeneity associated with European perch (Perca fluviatilis) predation on invasive round goby (Neogobius melanostomus).

    abstract::Spatiotemporal environmental variation affects fish feeding behaviour and capacity for piscivorous control of prey populations, which is important for management when prey include invasive species causing ecosystem impacts. We assessed gut-contents of an important piscivore (European perch Perca fluviatilis) over two ...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2017.10.017

    authors: Liversage K,Nurkse K,Kotta J,Järv L

    更新日期:2017-12-01 00:00:00

  • Vegetable oil spills on salt marsh sediments; comparison between sunflower and linseed oils.

    abstract::The effects of a simulated spill of sunflower oil in salt marsh sediments were compared with an experiment with linseed oil. Sunflower and linseed oil penetrated the sediments at the same rates but different adsorption of the oils onto sediment particles resulted in the establishment of anaerobic conditions at shallow...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/S0141-1136(02)00309-4

    authors: Pereira MG,Mudge SM,Latchford J

    更新日期:2003-09-01 00:00:00

  • The application of visual census on Mediterranean rocky habitats.

    abstract::In this study we examined the effect of: observer presence; observer speed; and the impact that multiple surveys had on the number of counted fish in a visual census survey in a typical Mediterranean rocky habitat. SCUBA observer presence had no influence on the number of fish per survey. An improvement in the methodo...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/s0141-1136(00)00028-3

    authors: De Girolamo M,Mazzoldi C

    更新日期:2001-02-01 00:00:00

  • Functional diversity of nematode communities in the southwestern North Sea.

    abstract::A range of biological traits of nematode species were combined to identify patterns in the functional composition of their assemblages collected at 19 soft-bottom stations in the southwestern North Sea with the primary aim to determine which environmental variables control communities. We used 19 categories of five bi...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2006.10.006

    authors: Schratzberger M,Warr K,Rogers SI

    更新日期:2007-05-01 00:00:00

  • Three dimensional water quality modeling of a shallow subtropical estuary.

    abstract::Knowledge of estuarine hydrodynamics and water quality comes mostly from studies of large estuarine systems. The processes affecting algae, nutrients, and dissolved oxygen (DO) in small and shallow subtropical estuaries are relatively less studied. This paper documents the development, calibration, and verification of...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/j.marenvres.2012.09.007

    authors: Wan Y,Ji ZG,Shen J,Hu G,Sun D

    更新日期:2012-12-01 00:00:00

  • Genotoxic, cytotoxic and developmental effects of tributyltin oxide (TBTO): an integrated approach to the evaluation of the relative sensitivities of two marine species.

    abstract::Adopting an integrated approach the potential genotoxic, cytotoxic and developmental effects of tributyltin oxide (TBTO), a known endocrine-disrupting agent for neogastropods, have been evaluated in two ecologically relevant invertebrates: Mytilus edulis (blue mussel) and Platynereis dumerilli (rag worm). Following de...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/s0141-1136(00)00112-4

    authors: Jha AN,Hagger JA,Hill SJ,Depledge MH

    更新日期:2000-07-01 00:00:00

  • Variability of heat shock proteins and glutathione S-transferase in gill and digestive gland of blue mussel, Mytilus edulis.

    abstract::Glutathione S-transferase (GST) and heat shock proteins (hsps) 40, 60, 70 and 90 were determined by immunoblotting using actin as an internal control in Mytilus edulis from one station outside (site1) and three stations within (sites 2-4) Cork Harbour, Ireland. Comparisons were made between gill and digestive gland an...

    journal_title:Marine environmental research

    pub_type: 杂志文章

    doi:10.1016/S0141-1136(03)00044-8

    authors: Lyons C,Dowling V,Tedengren M,Gardeström J,Hartl MG,O'Brien N,van Pelt FN,O'Halloran J,Sheehan D

    更新日期:2003-12-01 00:00:00