Abstract:
:We have previously shown that largemouth bass (Micropterus salmoides) has a remarkable ability to conjugate 4-hydroxy-2-nonenal (4HNE), a mutagenic and cytotoxic alpha,beta-unsaturated aldehyde produced during the peroxidation of lipids. In addition, we have isolated a glutathione S-transferase cDNA (bass GSTA) that encodes a recombinant protein which is highly active in 4HNE conjugation and structurally similar to plaice (Pleuronectes platessa) GSTA. In the present study, HPLC-GST subunit analysis revealed the presence of at least two major GST isoforms in bass liver, with one peak constituting 80% of the total bass liver GST protein. Liquid chromatography mass spectrometry (LC-MS) and electrospray ionization analysis of the major bass GST subunit yielded a molecular weight of 26,396 kDa. Endo-proteinase Lys-C digestion and Edman degradation protein sequencing of this GST peak demonstrated that this protein was encoded by bass GSTA. Analysis of genomic DNA fragments isolated by nested PCR indicated the presence of a GST gene cluster in bass liver that contained GSTA, and was similar to a GST gene cluster characterized by Leaver et al., in plaice. Collectively, our data indicates the presence of a major GST in bass liver involved in the protection against oxidative stress. This GST is part of a gene cluster that may be conserved in certain freshwater and marine fish.
journal_name
Mar Environ Resjournal_title
Marine environmental researchauthors
Pham RT,Barber DS,Gallagher EPdoi
10.1016/j.marenvres.2004.03.033keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
485-8issue
2-5eissn
0141-1136issn
1879-0291pii
S0141-1136(04)00084-4journal_volume
58pub_type
杂志文章abstract::Ocean acidification (OA) is predicted to affect the physiology of some fishes. To date, most studies have investigated this issue using stable pCO2 levels based on open ocean projections. Yet, most shallow, nearshore systems experience temporal and spatial pCO2 fluctuations. For example, pCO2 on coral reefs is highest...
journal_title:Marine environmental research
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abstract::Despite the pollution of microplastics being internationally recognized, the understanding of their behaviors in marine environment is still developing. Microplastics are ubiquitous in the marine environment, with the potential to cause harm to marine ecosystem. Here, we would classify the behaviors of microplastics a...
journal_title:Marine environmental research
pub_type: 杂志文章,评审
doi:10.1016/j.marenvres.2015.10.014
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abstract::The effects of sub-lethal concentrations of lindane on behavioral, hematological and histological parameters in Etroplus maculatus were investigated. The LC₅₀ value of lindane for E. maculatus was estimated using the Probit method and was found to be 0.028 mg/L. The red blood cell count (RBC) and the hemoglobin (Hb), ...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2011.10.011
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abstract::Concern over endocrine disrupters in coastal ecosystems has stimulated global efforts to understand their potential impacts on fish and invertebrate communities. Given that marine crustaceans are sensitive to the effects of alkylphenols and other xenobiotics, we are currently investigating whether these responses may ...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/s0141-1136(02)00113-7
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abstract::Most artificial reef (AR) studies have examined the early colonization stages of benthic communities, while only a few have monitored the development of AR communities beyond the initial successional phases and evaluated the time scale needed for such development. In addition, despite the proliferation of AR studies, ...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2004.03.122
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abstract::Juvenile turbot (Scophthalmus maximus) were injected intraperitoneally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled I and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydro...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/s0141-1136(02)00192-7
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abstract::Irregular sea urchins such as the spatangoid Spatangus purpureus are important bioturbators that contribute to natural biogenic disturbance and the functioning of biogeochemical cycles in soft sediments. In the coastal waters of the Balearic Islands S. purpureus occurs in soft red algal beds, and can reach high densit...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2011.01.008
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journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2018.04.012
更新日期:2018-08-01 00:00:00
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journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2019.104838
更新日期:2019-11-09 00:00:00
abstract::Habitat mapping is essential for the management and conservation of coastal marine habitats. However, accurate and up-to-date habitat maps are rarely available for the marine realm. In this study, we mapped the coastal marine habitats of >400 km of coastline in the north-western United Arab Emirates (UAE) using a comb...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2020.105095
更新日期:2020-10-01 00:00:00
abstract::Laeonereis acuta was seasonally collected in an industrially polluted site (P) and in an unpolluted site (UP) at the Patos Lagoon estuary (southern Brazil). Glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activity (U/mg protein) was determined in five groups of worms from each site. Meta...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2004.03.053
更新日期:2004-08-01 00:00:00
abstract::Ocean warming has multifaceted impacts on marine organisms. This study investigated the different responses of Paralichthys olivaceus and the hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic and acute heat stress. By comparing their survival, behavioural and histological changes, we found that the hybrids possess a...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2017.06.015
更新日期:2017-08-01 00:00:00
abstract::Microplastics are recognised as a (persistent) pollutant and are believed to be ubiquitous in the marine environment. The importance of this issue is evident from the large number of technical publications and research efforts within the past decade. However, the Arabian (Persian) Gulf region has few reported datasets...
journal_title:Marine environmental research
pub_type: 杂志文章,评审
doi:10.1016/j.marenvres.2020.104961
更新日期:2020-07-01 00:00:00
abstract::The aim of this study was to detect ecotoxicological effects of 0.1 μm polystyrene microbeads in marine organisms belonging to different trophic levels. MP build up, lethal and sub-lethal responses were investigated in the bacterium Vibrio anguillarum (culturability), in the green microalga Dunaliella tertiolecta (gro...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2018.09.023
更新日期:2018-10-01 00:00:00
abstract::The estuarine dynamics favoring the coexistence of mangroves, seagrass and corals at small river mouths are often poorly understood. We characterize the tidal, day/night and rainfall-induced short-term dynamics in salinity, pH, dissolved oxygen (DO), chlorophyll a (chl a), total suspended matter (TSM), water transpare...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2012.09.002
更新日期:2012-12-01 00:00:00
abstract::Autochthonous bioaugmentation for metal phytoremediation is still little explored, particularly its application to estuarine salt marshes, but results obtained so far are promising. Nevertheless, understanding the behaviour of the microbial communities in the process of bioaugmentation and their role in improving meta...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2017.10.007
更新日期:2017-12-01 00:00:00
abstract::The multixenobiotic resistance (MXR) mechanism, mediated by activity of the transmembrane P-glycoprotein, represents a basic biological defence system in aquatic organisms. Here we investigate the MXR transport activity in an aquatic vertebrate, the common carp (Cyprinus carpio). We measured the accumulation rate of a...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/s0141-1136(02)00155-1
更新日期:2002-09-01 00:00:00
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journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/s0141-1136(02)00130-7
更新日期:2002-09-01 00:00:00
abstract::Persistent organic pollutants (POPs), such as toxic lipophilic organochlorine (OC) compounds, accumulate in the blubber tissue of marine mammals. Toxicological sampling methods most frequently target only the superficial blubber layer. Vertical distribution of these contaminants through the blubber mantle may, however...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2013.11.004
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journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/s0141-1136(00)00060-x
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abstract::Field concentrations of polychlorinated biphenyls (PCBs) were measured in sediment and nine marine soft bottom invertebrate species. Lipid- and organic carbon normalised biota-sediment accumulation factors (BSAFs) were determined for 29 nonplanar and 11 coplanar congeners. To investigate whether the bioaccumulation wa...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2005.11.004
更新日期:2006-05-01 00:00:00
abstract::The Ria Formosa lagoon is an ecosystem whose water quality reflects the anthropogenic influence upon the surrounding areas. In this lagoon, the clam Ruditapes decussatus has a great economical importance and has been widely used as a biomonitor. A multibiomarker approach (δ-aminolevulinic acid dehydratase, metallothio...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2011.09.012
更新日期:2012-04-01 00:00:00
abstract::The amount of macroalgal biomass is an important ecosystem variable. Estimates can be made for a sampled area or values can be extrapolated to represent biomass over a larger region. Typically biomass is scaled-up using the area multiplied by the mean: a non-spatial method. Where algal biomass is patchy or shows gradi...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2020.104906
更新日期:2020-04-01 00:00:00
abstract::Spatiotemporal environmental variation affects fish feeding behaviour and capacity for piscivorous control of prey populations, which is important for management when prey include invasive species causing ecosystem impacts. We assessed gut-contents of an important piscivore (European perch Perca fluviatilis) over two ...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2017.10.017
更新日期:2017-12-01 00:00:00
abstract::The effects of a simulated spill of sunflower oil in salt marsh sediments were compared with an experiment with linseed oil. Sunflower and linseed oil penetrated the sediments at the same rates but different adsorption of the oils onto sediment particles resulted in the establishment of anaerobic conditions at shallow...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/S0141-1136(02)00309-4
更新日期:2003-09-01 00:00:00
abstract::In this study we examined the effect of: observer presence; observer speed; and the impact that multiple surveys had on the number of counted fish in a visual census survey in a typical Mediterranean rocky habitat. SCUBA observer presence had no influence on the number of fish per survey. An improvement in the methodo...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/s0141-1136(00)00028-3
更新日期:2001-02-01 00:00:00
abstract::A range of biological traits of nematode species were combined to identify patterns in the functional composition of their assemblages collected at 19 soft-bottom stations in the southwestern North Sea with the primary aim to determine which environmental variables control communities. We used 19 categories of five bi...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2006.10.006
更新日期:2007-05-01 00:00:00
abstract::Knowledge of estuarine hydrodynamics and water quality comes mostly from studies of large estuarine systems. The processes affecting algae, nutrients, and dissolved oxygen (DO) in small and shallow subtropical estuaries are relatively less studied. This paper documents the development, calibration, and verification of...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/j.marenvres.2012.09.007
更新日期:2012-12-01 00:00:00
abstract::Adopting an integrated approach the potential genotoxic, cytotoxic and developmental effects of tributyltin oxide (TBTO), a known endocrine-disrupting agent for neogastropods, have been evaluated in two ecologically relevant invertebrates: Mytilus edulis (blue mussel) and Platynereis dumerilli (rag worm). Following de...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/s0141-1136(00)00112-4
更新日期:2000-07-01 00:00:00
abstract::Glutathione S-transferase (GST) and heat shock proteins (hsps) 40, 60, 70 and 90 were determined by immunoblotting using actin as an internal control in Mytilus edulis from one station outside (site1) and three stations within (sites 2-4) Cork Harbour, Ireland. Comparisons were made between gill and digestive gland an...
journal_title:Marine environmental research
pub_type: 杂志文章
doi:10.1016/S0141-1136(03)00044-8
更新日期:2003-12-01 00:00:00