Analysis of telomerase activity and telomere length in bone and soft tissue tumors.

Abstract:

:Telomerase activation is prevalent in most epithelial tumors, and may be a critical step in cellular immortalization and carcinogenesis. However, telomerase activity in tumors of mesenchymal origin is not well understood. In the present study, we examined telomerase activity in clinical samples from osteosarcoma and soft tissue sarcoma and representative sarcoma cell lines (HOS, OST and Saos2), using the telomeric repeat amplification protocol (TRAP) assay. The cell lines HOS and OST were telomerase-positive, but Saos2 cells lacked telomerase activity and hTERT mRNA expression. Treatment of Saos2 cells with the demethylating agent 5-aza-2'-deoxy-cytidine, alone or together with the histone deacetylase inhibitor tricostatin A, did not induce hTERT mRNA expression. Twenty-six of the 83 sarcoma samples (31.3%) were telomerase-positive [bone sarcoma, 15 of 42 samples (35.7%); soft tissue sarcoma, 11 of 41 samples (26.8%)], whereas neither benign tumors nor normal bone tissue expressed telomerase activity. There was no significant correlation between histological type, tumor staging and telomerase activity. However, patients with telomerase-positive tumors had significantly shorter survival than those with telomerase-negative tumors. There was heterogeneity in telomere length (range, 6-18 kb) among the tumors examined, but there was no significant difference in length between telomerase-positive and -negative tumors. Thus, these mesenchymal tumors comprise heterologous groups, some positive and some negative for telomerase, with long and short telomeres, suggesting multiple carcinogenesis pathways. The present results indicate that telomerase activation is not prevalent in mesenchymal tumors and is not a critical determinant of telomere length, but it may be a prognostic indicator of mesenchymal tumors.

journal_name

Oncol Rep

journal_title

Oncology reports

authors

Terasaki T,Kyo S,Takakura M,Maida Y,Tsuchiya H,Tomita K,Inoue M

keywords:

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

1307-11

issue

6

eissn

1021-335X

issn

1791-2431

journal_volume

11

pub_type

杂志文章
  • Expression of immune checkpoint molecules of T cell immunoglobulin and mucin protein 3/galectin-9 for NK cell suppression in human gastrointestinal stromal tumors.

    abstract::Monoclonal antibody therapy for immune checkpoint blockade has achieved promising results for several types of malignant tumors. For the future treatment of gastrointestinal stromal tumors (GISTs) by immune checkpoint blockade, expression of immune checkpoint-related molecules that suppress antitumor immunity in GISTs...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4149

    authors: Komita H,Koido S,Hayashi K,Kan S,Ito M,Kamata Y,Suzuki M,Homma S

    更新日期:2015-10-01 00:00:00

  • Role of regenerative mucosa in primary gastric cancer treated by chemotherapy.

    abstract::We radiographically evaluated the response of primary lesions to chemotherapy in 101 patients with inoperable advanced gastric cancer. The median survival time in the 50 responders (49.5%) was 379 days, as compared with only 210 days in the 51 non-responders (50.1%) (p<0. 001). Morphologic changes of the gastric mucos...

    journal_title:Oncology reports

    pub_type: 临床试验,杂志文章

    doi:10.3892/or.7.6.1279

    authors: Yamazaki I,Koizumi W,Kondo I,Saigenji K

    更新日期:2000-11-01 00:00:00

  • Analysis of T-helper type 1 and 2 cells and T-cytotoxic type 1 and 2 cells of sentinel lymph nodes in breast cancer.

    abstract::The immune suppression of sentinel lymph node (SN) is directly influenced by primary tumors. It has been reported that the T-helper type 1 (Th1) to T-helper type 2 (Th2) ratio or T-cytotoxic type 1 (Tc1) to T-cytotoxic type 2 (Tc2) ratio of lymph node lymphocytes could be used to evaluate direct immunological circumst...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Ehi K,Ishigami S,Masamoto I,Uenosono Y,Natsugoe S,Arigami T,Arima H,Kijima Y,Yoshinaka H,Yanagita S,Kozono T,Funasako Y,Maruyama I,Aikou T

    更新日期:2008-03-01 00:00:00

  • Cannabinoids inhibit peptidoglycan-induced phosphorylation of NF-κB and cell growth in U87MG human malignant glioma cells.

    abstract::Nuclear factor (NF)-κB is the key transcription factor involved in the inflammatory responses, and its activation aggravates tumors. Peptidoglycan (PGN), a main cell wall component of Gram-positive bacteria, stimulates Toll-like receptor 2 (TLR-2) and activates a number of inflammat...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1937

    authors: Echigo R,Sugimoto N,Yachie A,Ohno-Shosaku T

    更新日期:2012-10-01 00:00:00

  • MicroRNA-128 suppresses cell growth and metastasis in colorectal carcinoma by targeting IRS1.

    abstract::Evidence has shown that microRNAs play important roles in tumor development, progression, and metastasis. miR-128 has been reported to be deregulated in different tumor types, whereas the function of miR-128 in colorectal carcinoma (CRC) largely remains to be elucidated. The aim of the present study was to investigate...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4251

    authors: Wu L,Shi B,Huang K,Fan G

    更新日期:2015-11-01 00:00:00

  • Gentian violet inhibits MDA-MB-231 human breast cancer cell proliferation, and reverses the stimulation of osteoclastogenesis and suppression of osteoblast activity induced by cancer cells.

    abstract::Gentian violet (GV) is a cationic triphenylmethane dye, with potent antifungal and antibacterial activity. We recently reported that in vitro GV suppresses the differentiation of bone resorbing osteoclasts while stimulating the differentiation and activity of bone forming osteoblasts. Breast cancer is highly metastati...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4190

    authors: Yamaguchi M,Vikulina T,Weitzmann MN

    更新日期:2015-10-01 00:00:00

  • miR-361-5p modulates metabolism and autophagy via the Sp1-mediated regulation of PKM2 in prostate cancer.

    abstract::Prostate cancer (PCa) is a leading cause of death among men. The dysregulation of metabolism and autophagy contributes to the progression of PCa. The transcription factor specificity protein 1 (Sp1) is implicated in the regulation of metabolism and autophagy. We confirmed that Sp1 is overexpressed in castration-resist...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5852

    authors: Ling Z,Liu D,Zhang G,Liang Q,Xiang P,Xu Y,Han C,Tao T

    更新日期:2017-09-01 00:00:00

  • Survivin overexpression in head and neck squamous cell carcinomas as a new therapeutic target (Review).

    abstract::Head and neck squamous cell carcinoma (HNSCC) is the sixth most commonly diagnosed cancer worldwide. It has poor clinical outcome due to intrinsic or acquired drug resistance. Deregulation of both apoptosis and autophagy contributes to chemotherapy resistance and disease progression. A new member of the inhibitors of ...

    journal_title:Oncology reports

    pub_type: 杂志文章,评审

    doi:10.3892/or.2019.7082

    authors: Frassanito MA,Saltarella I,Vinella A,Muzio LL,Pannone G,Fumarulo R,Vacca A,Mariggiò MA

    更新日期:2019-05-01 00:00:00

  • Predictive factors in patients with advanced and metastatic squamous cell carcinoma of the head and neck: a study based on SWOG protocol S0420.

    abstract::To evaluate the prognostic values of different protein expression in the progression of squamous cell carcinoma of the head and neck (SCCHN) patients, we conducted immunohistochemical (IHC) analysis in tissue samples of different patients enrolled on SWOG protocol S0420. S0420 was a phase II trial to evaluate the effi...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2374

    authors: Mehta S,Moon J,Hashmi M,Leblanc M,Huang CH,Rinehart E,Wolf GT,Urba SG,Banerjee SK,Williamson S

    更新日期:2013-06-01 00:00:00

  • Peroxiredoxin 1 is a tumor-associated antigen in esophageal squamous cell carcinoma.

    abstract::Peroxiredoxin 1 (Prdx1) is an antioxidant and plays an important role in H2O2-mediated cell signaling. We previously found that the expression level of Prdx1 was elevated in esophagus squamous cell carcinoma (ESCC) tissue using a proteomics approach. Since overexpressed protein can induce an autoimmune response, to fu...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2714

    authors: Ren P,Ye H,Dai L,Liu M,Liu X,Chai Y,Shao Q,Li Y,Lei N,Peng B,Yao W,Zhang J

    更新日期:2013-11-01 00:00:00

  • Quercetin acts as an antioxidant and downregulates CYP1A1 and CYP1B1 against DMBA-induced oxidative stress in mice.

    abstract::We investigated the effects of quercetin on 7,12-dimethylbenz(a)anthracene (DMBA)-induced oxidative stress and the expression of CYP1A1 and CYP1B1 in mice. Quercetin was administered orally to mice at 100 or 250 mg/kg BW for 18 days, after which DMBA (34 mg/kg BW) was administered intragastrically twice. Quercetin sho...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1753

    authors: Choi EJ,Kim T,Kim GH

    更新日期:2012-07-01 00:00:00

  • Activation of PAR2 or/and TLR4 promotes SW620 cell proliferation and migration via phosphorylation of ERK1/2.

    abstract::Protease-activated receptor 2 (PAR2) is a G-protein-coupled receptor and its activation has been associated with the pathogenetic progress in certain cancers. Toll-like receptor 4 (TLR4), one member of Toll-like receptors family, is mainly contributed to the innate immune response. However, recent studies have shown t...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2010.1077

    authors: Zhou B,Zhou H,Ling S,Guo D,Yan Y,Zhou F,Wu Y

    更新日期:2011-02-01 00:00:00

  • Significance of glucose intolerance and SHIP2 expression in hepatocellular carcinoma patients with HCV infection.

    abstract::Glucose intolerance frequently is found in hepatocellular carcinoma (HCC) patients with hepatitis C virus (HCV) infection; however, the significance of glucose intolerance remains unclear. In addition, SH2 domain-containing inositol phosphatase (SHIP) 2 is a negative regulator of intracellular insulin signaling; howev...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Sumie S,Kawaguchi T,Komuta M,Kuromatsu R,Itano S,Okuda K,Taniguchi E,Ando E,Takata A,Fukushima N,Koga H,Torimura T,Kojiro M,Sata M

    更新日期:2007-09-01 00:00:00

  • lncRNA NEAT1 promotes cell proliferation and invasion by regulating miR‑365/RGS20 in oral squamous cell carcinoma.

    abstract::Long non‑coding RNAs (lncRNAs) have emerged as critical regulators of tumor progression. However, the function and mechanism of lncRNA NEAT1 in oral squamous cell carcinoma (OSCC) are unclear. In the present study, NEAT1 was significantly upregulated in OSCC cells and tissues. High expression of NEAT1 was correlated w...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6283

    authors: Huang G,He X,Wei XL

    更新日期:2018-04-01 00:00:00

  • Lung cancer patients with chronic obstructive pulmonary disease.

    abstract::The aim of this study is to evaluate characteristics in lung cancer patients with chronic obstructive pulmonary disease (COPD). Among 966 lung cancer patients admitted to our division over a period of 24 years, 73 patients were diagnosed as having COPD. There were 68 (93.2%) men and 5 women; of the tumors 43 (58.9%) w...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Kurishima K,Satoh H,Ishikawa H,Yamashita YT,Homma T,Ohtsuka M,Sekizawa K

    更新日期:2001-01-01 00:00:00

  • O6-methylguanine-DNA methyltransferase promoter hypermethylation in colorectal carcinogenesis.

    abstract::Epigenetic alterations have been reported in colorectal neoplasia which can either complement or in some cases be predisposed to genetic alterations such as K-ras mutations. We examined the promoter methylation status of the CDKN2A and O6-methylguanine-DNA methyltransferase (MGMT) genes, after sodium bisulfite convers...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Menigatti M,Pedroni M,Verrone AM,Borghi F,Scarselli A,Benatti P,Losi L,Di Gregorio C,Schär P,Marra G,Ponz de Leon M,Roncucci L

    更新日期:2007-06-01 00:00:00

  • MYC promotes the development of papillary thyroid carcinoma by inhibiting the expression of lncRNA PAX8‑AS1:28.

    abstract::As a common malignancy of the endocrine system, papillary thyroid carcinoma (PTC) seriously affects the quality of life of patients. lncRNA PAX8‑AS1:28, or lnc‑PSD4‑1:14 has been reported to be abnormally expressed in PTC. However, the function of PAX8‑AS1:28 in PTC is still unknown. Therefore, the present study aimed...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.6996

    authors: Zhang Y,Li F,Chen J

    更新日期:2019-04-01 00:00:00

  • C5a promotes the proliferation of human nasopharyngeal carcinoma cells through PCAF-mediated STAT3 acetylation.

    abstract::The anaphylatoxin C5a is a chemoattractant that can induce various inflammatory responses in vivo via the C5a receptor (C5aR). There is emerging evidence that C5a is generated in the cancer microenvironment. However, the role of C5a in human nasopharyngeal carcinoma (NPC) remains largely unclear. Thus, the present stu...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3420

    authors: Cai K,Wan Y,Wang Z,Wang Y,Zhao X,Bao X

    更新日期:2014-11-01 00:00:00

  • Expression and activation of P38 MAP kinase in invasive ductal breast cancers: correlation with expression of the estrogen receptor, HER2 and downstream signaling phosphorylated proteins.

    abstract::MAP kinase signaling proteins have major implications in the molecular oncogenesis of breast cancers and have been extensively investigated as putative targets for therapy. This study reports the investigation of the expression of P38 MAPK and its phosphorylated form (p-P38 MAPK) in clinical specimens of invasive brea...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2645

    authors: Merlin JL,Harlé A,Lion M,Ramacci C,Leroux A

    更新日期:2013-10-01 00:00:00

  • Aberrant expression of beta-catenin, Pin1 and cylin D1 in salivary adenoid cystic carcinoma: relation to tumor proliferation and metastasis.

    abstract::The aims of this study were to investigate the expression levels of beta-catenin, Pin1 and cyclin D1 in salivary adenoid cystic carcinomas (SACC ) and to evaluate its clinical importance, furthermore, to elucidate whether beta-catenin expression was aberrant in SACC and whether Pin1 was involved in aberrant beta-caten...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Zhou CX,Gao Y

    更新日期:2006-09-01 00:00:00

  • Characterizing the molecular mechanisms of acquired temozolomide resistance in the U251 glioblastoma cell line by protein microarray.

    abstract::Acquired chemoresistance refers to tumor cells gradually losing their sensitivity to anticancer drugs during the course of treatment, resulting in tumor progression or recurrence. This phenomenon, which has deleterious outcomes for the patient, has long been observed in patients with glioblastoma receiving temozolomid...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6322

    authors: Lin J,Zuo J,Cui Y,Song C,Wu X,Feng H,Li J,Li S,Xu Q,Wei W,Qiu G,He H

    更新日期:2018-05-01 00:00:00

  • Connexins 26 and 43 correlate with Bak, but not with Bcl-2 protein in breast cancer.

    abstract::Apoptosis is a process associated with development and progression of breast cancer. The association between connexins and programmed cell death has only been demonstrated in a few studies. The aim of the present study was the evaluation of Cx26 and Cx43 expression in breast cancer in correlation with Bcl-2 and Bak pr...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Kanczuga-Koda L,Sulkowski S,Tomaszewski J,Koda M,Sulkowska M,Przystupa W,Golaszewska J,Baltaziak M

    更新日期:2005-08-01 00:00:00

  • Sodium valproate, a histone deacetylase inhibitor, augments the expression of cell-surface NKG2D ligands, MICA/B, without increasing their soluble forms to enhance susceptibility of human osteosarcoma cells to NK cell-mediated cytotoxicity.

    abstract::MHC class I-related chain molecules A and B (MICA and B) expressed on the cell-surface of tumor cells are ligands for an activating receptor, NKG2D, expressed on natural killer (NK) cells and stimulate the NK cell-mediated cytotoxicity. On the other hand, the soluble form of MICA and B produced by proteolytic cleavage...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or_00001026

    authors: Yamanegi K,Yamane J,Kobayashi K,Kato-Kogoe N,Ohyama H,Nakasho K,Yamada N,Hata M,Nishioka T,Fukunaga S,Futani H,Okamura H,Terada N

    更新日期:2010-12-01 00:00:00

  • Exploring the role of glucose‑6‑phosphate dehydrogenase in cancer (Review).

    abstract::Glucose‑6‑phosphate dehydrogenase (G6PD) is a cytoplasmic enzyme found in human erythrocytes that provides reduced NADPH for cell metabolism. Glutathione produced by the G6PD pathway can reduce the degree of harm caused by reactive oxygen species such as oxygen‑containing free radicals, peroxides and lipid peroxides. ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2020.7803

    authors: Li R,Wang W,Yang Y,Gu C

    更新日期:2020-12-01 00:00:00

  • Dihydropyrimidine dehydrogenase inhibitory fluoropyrimidine S-1 may be effective against peritoneal dissemination in gastric cancer.

    abstract::The effects of a novel oral fluoropyrimidine derivative S-1 on peritoneal metastasis from gastric cancer were investigated. OCUM-2MD3 cells, a highly peritoneal-metastatic cell line, were injected intraperitoneally in nude mice. These mice were allocated to the following three groups (each group, n=10): the S-1 group,...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Yamagata S,Nakata B,Hirakawa K

    更新日期:2004-11-01 00:00:00

  • Effects of miR‑181a on the biological function of multiple myeloma.

    abstract::The present study aimed to investigate the role of miR‑181a in multiple myeloma (MM) cell lines. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect the expression of microRNA (miR)‑181a. The MM cell line RPMI 8226 stably transduced with miR‑181a mimics or inhibitor was establishe...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7160

    authors: Liu N,Yang J,Yuan R,Peng J,Liu L,Guo X

    更新日期:2019-07-01 00:00:00

  • Aged garlic extract inhibits 1,2-dimethylhydrazine-induced colon tumor development by suppressing cell proliferation.

    abstract::Garlic and its constituents are reported to have a preventive effect against colorectal cancer in animal models. Aged garlic extract (AGE), which is produced by natural extraction from fresh garlic for more than 10 months in aqueous ethanol, also has reputed chemopreventive effects on colon carcinogenesis, but has nev...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3705

    authors: Jikihara H,Qi G,Nozoe K,Hirokawa M,Sato H,Sugihara Y,Shimamoto F

    更新日期:2015-03-01 00:00:00

  • Angiotensin II receptor type 1 blockers suppress the cell proliferation effects of angiotensin II in breast cancer cells by inhibiting AT1R signaling.

    abstract::Chronic stress and a high-fat diet are well-documented risk factors associated with the renin-angiotensin system in the development of breast cancer. The angiotensin II type 1 receptor (AT1R) is a novel component of the renin-angiotensin system. Several recent studies have focused on the function of AT1R in cell proli...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1720

    authors: Du N,Feng J,Hu LJ,Sun X,Sun HB,Zhao Y,Yang YP,Ren H

    更新日期:2012-06-01 00:00:00

  • Oxymatrine inhibits epithelial-mesenchymal transition through regulation of NF-κB signaling in colorectal cancer cells.

    abstract::Oxymatrine, a traditional Chinese herb extracted from Sophora flavescens Ait., displays strong anti-inflammatory and anticancer activities, but how oxymatrine exhibits anticarcinogenic effects in human colorectal cancer (CRC) remains uncertain. The present study aimed to elucidate the exact mechanism by which oxymatri...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4927

    authors: Liang L,Huang J

    更新日期:2016-09-01 00:00:00

  • Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells.

    abstract::DNA double-strand breaks (DSBs) induced by ionizing radiation (IR) are among the most cytotoxic types of DNA damage. The DNA damage response (DDR) may be a reason for the cancer cell resistance to radiotherapy using IR. Identified as critical upstream mediators of the phosphorylation of ataxia telangiectasia-mutated (...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.3980

    authors: Gou Q,Xie Y,Liu L,Xie K,Wu Y,Wang Q,Wang Z,Li P

    更新日期:2015-07-01 00:00:00