Abstract:
:The thermoacidophile crenarchaeote Sulfolobus ssp. is one of the best-studied Archaea. Cryptic and conjugative plasmids as well as viruses have been described for this genus. For the majority of the genetic elements only the genome sequence and the basic characteristics were determined. In contrast the fusellovirus SSV1 and the cryptic plasmid pRN1, which is the smallest known genetic element of the crenarchaeota, have been studied in more detail. The three gene products of the plasmid pRN1 have been characterized biochemically. The replication protein of the plasmid, a multifunctional enzyme, has a novel domain, termed prim/pol domain. This domain constitutes the first member of the DNA polymerase family E. Based on the biochemical characterization of the gene products a model of how pRN1 is replicated in vivo is proposed.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Lipps Gdoi
10.1042/bst0320240keywords:
subject
Has Abstractpub_date
2004-04-01 00:00:00pages
240-4issue
Pt 2eissn
0300-5127issn
1470-8752journal_volume
32pub_type
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