Human papillomavirus type specific DNA and RNA persistence--implications for cervical disease progression and monitoring.

Abstract:

:In 2000, we monitored the course and persistence of human papillomavirus (HPV) infection in 54 women who were HPV positive and free of any cytological disease using HPV-DNA genotyping with a linear array assay (baseline). The impact of HPV infection on development of cervical cytological abnormality (dyskaryosis) was monitored by repeat HPV genotyping and cytological assessment 2 years later. Detection of mRNA transcripts of known HPV oncogenes E6 and E7 using NASBA methodology and specific molecular beacons for five common HPV types was also performed at both time points. A total of 11/54 (20%) women developed dyskaryosis after 2 years with 31/54 and 23/54 women exhibiting transient and persistent infections respectively, as monitored by DNA genotyping. Women who maintained type-specific persistent HPV infection were significantly more likely to develop dyskaryosis compared to those who exhibited a transient infection (P = 0.001). The presence of HPV mRNA E6/E7 transcripts was less sensitive but more specific for the detection of disease at follow up. Moreover, women who were DNA positive and also positive for mRNA transcripts at baseline were significantly more likely to harbour persistent infection compared to those in whom DNA only was detected at baseline (P = 0.013). This study highlights the importance of detecting persistent type specific HPV infection to identify those women more at risk of developing cervical abnormalities, either by repeated DNA genotyping, or potentially by RNA based techniques that may be more predictive of persistent infection if performed at a single time point.

journal_name

J Med Virol

authors

Cuschieri KS,Whitley MJ,Cubie HA

doi

10.1002/jmv.20062

keywords:

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

65-70

issue

1

eissn

0146-6615

issn

1096-9071

journal_volume

73

pub_type

杂志文章
  • Increased replication of respiratory syncytial virus in the presence of cytokeratin 8 and 18.

    abstract::Previously, it was reported that productive viral infection, viral protein synthesis, and viral RNA replication of respiratory syncytial virus (RSV) operated efficiently in two human epithelial cell lines (HEp-2 and A549), but not in a human mast-cell line, HMC-1. Based on these observations, it was hypothesized that ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23196

    authors: Shirato K,Ujike M,Kawase M,Matsuyama S

    更新日期:2012-02-01 00:00:00

  • Misidentification of recombinant hepatitis C virus leading to treatment failure with direct acting antivirals.

    abstract::The use of new Direct Acting Antivirals, specific of HCV, has greatly improved the HCV treatment. Most of the DAA are specific of HCV genotypes. Genotyping methods may target different regions of the HCV genome, though only the whole genome sequencing could confirm the correct genotype. The present study describes the...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25035

    authors: Mourez T,Decroos A,Goria O,Montialoux H,De Oliveira F,Larrat S,Plantier JC,Riachi G

    更新日期:2018-05-01 00:00:00

  • Relative frequency of human rotavirus subgroups 1 and 2 in Japanese children with acute gastroenteritis.

    abstract::Recently developed monoclonal antibodies against the 42,000-dalton major inner capsid protein were used in an enzyme immunoassay to subgroup a total of 156 rotavirus specimens obtained from Japanese infants and young children with acute gastroenteritis during the period between December 1981 and April 1983. Only 2 spe...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890170105

    authors: Nakagomi O,Nakagomi T,Oyamada H,Suto T

    更新日期:1985-09-01 00:00:00

  • Identification of human astrovirus infections among children with acute gastroenteritis in the Southern Part of Vietnam during 2005-2006.

    abstract::A molecular epidemiological study on common diarrheal viruses was conducted in a children's hospital in Ho Chi Minh City between December 2005 and November 2006. Fecal samples were collected from 502 pediatric patients with acute gastroenteritis, and were screened for eight types of viral agents. Human astroviruses (H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21032

    authors: Nguyen TA,Hoang L,Pham le D,Hoang KT,Mizuguchi M,Okitsu S,Ushijima H

    更新日期:2008-02-01 00:00:00

  • Temporal distribution of human rotavirus serotypes 1,2,3, and 4 in Venezuelan children with gastroenteritis during 1979-1989.

    abstract::The temporal distribution and clinical severity of rotavirus VP7 serotypes 1, 2, 3, and 4 recovered from 427 Venezuelan children with acute gastroenteritis over a period of 11 years were studied. Rotavirus VP7 serotype was established by ELISA serotyping in 298 (69.78%) of the specimens while the serotype of the remai...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890340202

    authors: White L,García D,Boher Y,Blanco M,Pérez M,Romer H,Flores J,Pérez-Schael I

    更新日期:1991-06-01 00:00:00

  • Comparison of characteristics between patients with GB virus C/hepatitis G virus (GBV-C/HGV) RNA and those with GBV-C/HGV E2-antibody in patients with hemophilia.

    abstract::We compared characteristics of patients with GB virus C/hepatitis G virus (GBV-C/HGV) RNA to those of patients with GBV-C/HGV E2-antibody. GBV-C/HGV RNA and GBV-C/HGV antibody were assayed in 83 persons with hemophilia using a reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay, re...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(200001)60:1<34::aid-jmv6>3

    authors: Toyoda H,Takahashi I,Fukuda Y,Hayakawa T,Takamatsu J

    更新日期:2000-01-01 00:00:00

  • Allele-specific conventional reverse-transcription polymerase chain reaction as a screening assay for discriminating influenza a H1N1 (H275Y) oseltamivir-resistant and wild-type viruses.

    abstract::In early 2008, a sudden increase in oseltamivir (Tamiflu)-resistant influenza A H1N1 viruses was reported from several European countries. This resistant virus has spread globally and accounted for more than 95% of H1N1 viruses isolated in the following influenza season. A continuous close monitoring on the prevalence...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21783

    authors: Ngai KL,Lam WY,Lee N,Leung TF,Hui DS,Chan PK

    更新日期:2010-08-01 00:00:00

  • Different patterns of cytokine induction in cultures of respiratory syncytial (RS) virus-specific human TH cell lines following stimulation with RS virus and RS virus proteins.

    abstract::Human peripheral blood mononuclear cell (PBMC) proliferative responses to live respiratory syncytial (RS) virus, formalin-inactivated RS (Fl-RS) virus, RS virus F (fusion) protein, and RS virus G (attachment) protein were assessed. All donors responded to challenge with whole RS virus antigens and F and G proteins. F ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1096-9071(199607)49:3<161::AID-JMV2>

    authors: Jackson M,Scott R

    更新日期:1996-07-01 00:00:00

  • Selection of drug-resistant HIV-1 during the early phase of viral decay is uncommon in treatment-naïve patients initiated on a three- or four-drug antiretroviral regimen including lamivudine.

    abstract::Therapy failure due to drug resistance development is a common phenomenon in HIV-infected patients. However, when the drug pressure leads to the earliest selection of drug-resistant HIV-1 populations is still unclear. In this study, the extent to which selection of the HIV-1 reverse transcriptase M184I/V mutations occ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21363

    authors: Bergroth T,Ekici H,Gisslén M,Loes SK,Goh LE,Freedman A,Lampe F,Johnson MA,Sönnerborg A

    更新日期:2009-01-01 00:00:00

  • Human and monoclonal antibodies to hepatitis B core antigen recognise a single immunodominant epitope.

    abstract::Monoclonal antibodies were raised against hepatitis B core antigen (HBcAg) synthesized in Escherichia coli. They identified a single immunodominant determinant on both liver-derived and E. coli-derived HBcAg. Cross-inhibition studies demonstrated that HBsAg-containing human sera which contained antibodies to HBcAg (an...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890190213

    authors: Ferns RB,Tedder RS

    更新日期:1986-06-01 00:00:00

  • Human parechovirus infections in patients admitted to hospital in Northern Italy, 2008-2010.

    abstract::Human parechoviruses (HPeVs) infection is associated with a wide range of clinical syndromes such as respiratory, gastrointestinal, neurologic diseases, and neonatal sepsis-like illness. The main objective of this study was to investigate the epidemiology of HPeVs infection in hospitalized patients in a period of 2 ye...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23197

    authors: Piralla A,Furione M,Rovida F,Marchi A,Stronati M,Gerna G,Baldanti F

    更新日期:2012-04-01 00:00:00

  • Cross-species hybridization: characterization of gene expression in woodchuck liver using human membrane arrays.

    abstract::Total RNA from normal adult woodchucks was analyzed using membrane arrays containing human cDNA clones, and the gene expression patterns were compared to human liver. Various hybridization and wash conditions were examined. In both the woodchuck and human livers, 352 genes were identified as highly expressed (Z-scores...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20186

    authors: Rinaudo JA,Gerin JL

    更新日期:2004-10-01 00:00:00

  • Genetic heterogeneity in the precore region of hepatitis B virus in hepatitis B e antigen-negative chronic hepatitis B patients: spontaneous seroconversion and interferon-induced seroconversion.

    abstract::To elucidate the relationship between the clinical severity of chronic liver disease and the precore mutations in hepatitis B e antigen (HBeAg)-negative hepatitis B virus (HBV) carriers, mutations were investigated in the precore region of HBV DNA in 20 chronic hepatitis B patients who seroconverted either spontaneous...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890450404

    authors: Karasawa T,Aizawa Y,Zeniya M,Kuramoto A,Shirasawa T,Toda G

    更新日期:1995-04-01 00:00:00

  • Detection of respiratory syncytial virus by RNA-polymerase chain reaction and differentiation of subgroups with oligonucleotide probes.

    abstract::The polymerase chain reaction (RNA-PCR) was used for specific detection of respiratory syncytial virus (RSV) genomes in clinical specimens. A set of primers was selected from conserved regions of the 1B and N genes for detection of both subgroups. The primers were found to be RSV specific, all RSV strains generated a ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890440115

    authors: van Milaan AJ,Sprenger MJ,Rothbarth PH,Brandenburg AH,Masurel N,Claas EC

    更新日期:1994-09-01 00:00:00

  • Sequence and structural analysis of the 5' noncoding region of hepatitis C virus in patients with chronic infection.

    abstract::Hepatitis C virus (HCV), exhibits considerable genetic diversity, but presents a relatively well conserved 5' noncoding region (5' NCR) among all genotypes. In this study, the structural features and translational efficiency of the HCV 5' NCR sequences were analyzed using the programs RNAfold, RNAshapes and RNApdist a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21507

    authors: Araújo FM,Machado-Lima A,Durham AM,Teixeira R,Oliveira G

    更新日期:2009-07-01 00:00:00

  • Development and validation of a new serum standard for the measurement of anti-HEV antibodies in animals.

    abstract::Although hepatitis E virus (HEV) infection is a zoonosis, there is, currently, no standardized assay for quantitatively measuring anti-HEV antibody levels in HEV animal reservoirs. In this study, anti-HEV antibody positive serum from a rabbit (RS26) was calibrated by dose-response parallel line assay using the World H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24651

    authors: Zhang Y,Wang L,Zeng H,Gong W,Wang L,Zhuang H

    更新日期:2017-03-01 00:00:00

  • Multicenter randomized study comparing initial daily induction with high dose lymphoblastoid interferon vs. standard interferon treatment for chronic hepatitis C.

    abstract::One hundred fifty-five chronic hepatitis C patients were assigned at random to receive natural lymphoblastoid interferon (IFN)alpha-n1, s.c., for 13 months in one of three treatment regimens: initial daily induction with 10 million units (MU) followed (group 1, n = 50) or not (group 2, n = 52) by 1 month of rest and t...

    journal_title:Journal of medical virology

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1002/jmv.1072

    authors: Diago M,Suárez D,García-Villarreal L,Castro A,Domínguez A,Pardo M,del Olmo JA,Pérez-Hernández F,Aguilar J,Quiroga JA,Carreño V

    更新日期:2001-08-01 00:00:00

  • Genome types of adenovirus type 7 isolated in Hiroshima City.

    abstract::One hundred thirty-two strains of adenovirus type 7 were isolated in Hiroshima City during the period 1995-1999. About 80% of the viruses were isolated from patients with respiratory illness, and about 70% were isolated from children younger than 6 years old. DNA restriction analysis was performed on 58 of the 132 iso...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10287

    authors: Ikeda Y,Yamaoka K,Noda M,Ogino T

    更新日期:2003-02-01 00:00:00

  • Molecular and epidemiological characteristics of blood-borne virus infections among recent immigrants in Spain.

    abstract::The increased immigration from developing regions to Western countries raises public health concerns related to blood-borne viruses. The prevalence of human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), and human T-lymphotropic virus (HTLV) infections among recent immigrants attending...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20744

    authors: Toro C,Jiménez V,Rodríguez C,Del Romero J,Rodés B,Holguín A,Alvarez P,García-Campello M,Gómez-Hernando C,Guelar A,Sheldon J,de Mendoza C,Simón A,Soriano V

    更新日期:2006-12-01 00:00:00

  • Correlation of HTLV-1 Tax genetic diversity with HTLV-1 associated myelopathy/tropical spastic paraparesis progression and HTLV-1a genotypes in an HTLV-1 endemic region in Argentina.

    abstract::The oncoprotein Tax was characterized genetically from a large cohort of human T-cell lymphotropic virus type 1 (HTLV-1) seropositive individuals from the most endemic region of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and HTLV-1 infection in Argentina, the province of San Salvador de Jujuy....

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21833

    authors: Iñiguez AM,Gastaldello R,Otsuki K,Balangero M,Carvalho Costa F,Remondegui C,Paula Vicente AC,Gallego S

    更新日期:2010-08-01 00:00:00

  • Association of mortality and recent Mycoplasma pneumoniae infection in COVID-19 patients.

    abstract::To compare characteristics and outcomes of patients who had COVID-19 with Mycoplasma pneumoniae immunoglobulin M (IgM) antibodies to those without M. pneumoniae antibodies. We retrospectively reviewed cases admitted over a 4-week period between 17 March 2020 and 14 April 2020 to the Hoboken University Medical Center, ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26467

    authors: Amin D,McKitish K,Shah PS

    更新日期:2021-02-01 00:00:00

  • Prognostic value of adenosine deaminase compared to other markers for progression to acquired immunodeficiency syndrome among intravenous drug users.

    abstract::This study was designed to determine the prognostic value of erythrocyte adenosine deaminase (ADA) as a possible indicator of progression to AIDS, and compare this with other known cellular and serological markers. At the end of a 3-year study, a cohort of 114 human immunodeficiency virus-1 (HIV-1) seropositive intrav...

    journal_title:Journal of medical virology

    pub_type: 临床试验,杂志文章

    doi:10.1002/jmv.1890450216

    authors: Casoli C,Lisa A,Magnani G,Starcich R,Fiaccadori F,Bertazzoni U,Zei G

    更新日期:1995-02-01 00:00:00

  • Molecular and epidemiological study on nosocomial transmission of HCV in hemodialysis patients in Brazil.

    abstract::An epidemiological and molecular study of hepatitis C virus (HCV) infection was carried out in Brazilian hemodialysis centers. A total of 1,095 patients in all 15 hemodialysis centers in the State of Goiás, Brazil, were studied. All patients were interviewed for possible risk factors to HCV infection and serum samples...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20932

    authors: Carneiro MA,Teles SA,Lampe E,Espírito-Santo MP,Gouveia-Oliveira R,Reis NR,Yoshida CF,Martins RM

    更新日期:2007-09-01 00:00:00

  • Molecular epidemiology of HIV-1 among the HIV infected people of Manipur, Northeastern India: Emergence of unique recombinant forms.

    abstract::According to the Joint National Programme on HIV/AIDS (UNAIDS), the northeastern region of India has the highest HIV prevalence in the country. This study was conducted to determine the current HIV-1 molecular epidemiology of Manipur, a state in northeast India. Blood samples from HIV-1 seropositive subjects were coll...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24738

    authors: Sharma AL,Singh TR,Devi KR,Singh LS

    更新日期:2017-06-01 00:00:00

  • Virological patterns of HCV patients with failure to interferon-free regimens.

    abstract::The study characterized the virological patterns and the resistance-associated substitutions (RASs) in patients with failure to IFN-free regimens enrolled in the real-life setting. All 87 consecutive HCV patients with failed IFN-free regimens, observed at the laboratory of the University of Campania, were enrolled. Al...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25022

    authors: Starace M,Minichini C,De Pascalis S,Macera M,Occhiello L,Messina V,Sangiovanni V,Adinolfi LE,Claar E,Precone D,Stornaiuolo G,Stanzione M,Ascione T,Caroprese M,Zampino R,Parrilli G,Gentile I,Brancaccio G,Iovinella V,

    更新日期:2018-05-01 00:00:00

  • Fulminant demyelinating encephalomyelitis associated with productive HHV-6 infection in an immunocompetent adult.

    abstract::Human herpesvirus 6 (HHV-6), the etiologic agent of roseola in young children, has been reported to be detectable in the brain of many neurologically normal adults, although regional localization to plaques of multiple sclerosis has also been demonstrated. Large amounts of this virus were present in multifocal demyeli...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199707)52:3<301::aid-jmv11

    authors: Novoa LJ,Nagra RM,Nakawatase T,Edwards-Lee T,Tourtellotte WW,Cornford ME

    更新日期:1997-07-01 00:00:00

  • Induction of apoptosis in HT-29 cells infected with SA-11 rotavirus.

    abstract::Rotavirus infection is associated both in vivo and in vitro with a series of subcellular pathological alterations leading to cell lysis. It has been suggested that these modifications can play a key role in the pathogenesis of rotavirus-associated diarrheal disease. We describe the effects of SA-11 rotavirus infection...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1096-9071(199612)50:4<325::AID-JMV8>

    authors: Superti F,Ammendolia MG,Tinari A,Bucci B,Giammarioli AM,Rainaldi G,Rivabene R,Donelli G

    更新日期:1996-12-01 00:00:00

  • Low HBV-DNA levels in end-stage renal disease patients with HBeAg-negative chronic hepatitis B.

    abstract::In end-stage renal disease patients treated by hemodialysis with HBeAg-negative chronic hepatitis B virus (HBV) infection, the evaluation of the presence of viral replication is essential in the assessment for renal transplantation. Data on HBV viral load, prevalence of precore mutations, as well as the influence of H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20691

    authors: Moutinho RS,Perez RM,Medina-Pestana JO,Figueiredo MS,Koide S,Alberto FL,Silva AE,Ferraz ML

    更新日期:2006-10-01 00:00:00

  • A simple method for detection of a novel coronavirus (SARS-CoV-2) using one-step RT-PCR followed by restriction fragment length polymorphism.

    abstract::A novel coronavirus associated with acute respiratory disease (named SARS-CoV-2) is recently identified in Wuhan city, China, spread rapidly worldwide. Early identification of this novel coronavirus by molecular tools is critical for surveillance and control of the epidemic outbreak. We aimed to establish a simple met...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26171

    authors: Son HA,Hang DTT,Thuan ND,Quyen LTB,Thuong LTH,Nga VT,Quang LB,Hung TT,Son NT,Linh NT,Nam LV,Van Ba N,Tien TV,Quyet D,Van Luong H,Su HX

    更新日期:2020-11-01 00:00:00

  • Tula and Puumala hantavirus NSs ORFs are functional and the products inhibit activation of the interferon-beta promoter.

    abstract::The S RNA genome segment of hantaviruses carried by Arvicolinae and Sigmodontinae rodents encodes the nucleocapsid (N) protein and has an overlapping (+1) open reading frame (ORF) for a putative nonstructural protein (NSs). The aim of this study was to determine whether the ORF is functional. A protein corresponding t...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20948

    authors: Jääskeläinen KM,Kaukinen P,Minskaya ES,Plyusnina A,Vapalahti O,Elliott RM,Weber F,Vaheri A,Plyusnin A

    更新日期:2007-10-01 00:00:00