Abstract:
:The RNA polymerase beta-subunit genes (rpoB) of 67 Streptomyces strains, representing 57 species, five Kitasatospora strains and Micromonospora echinospora KCTC 9549 were partially sequenced using a pair of rpoB PCR primers. Among the streptomycetes, 99.7-100 % similarity within the same species and 90.2-99.3 % similarity at the interspecific level were observed by analysis of the determined rpoB sequences. The topology of the phylogenetic tree based on rpoB sequences was similar to that of 16S rDNA. The five Kitasatospora strains formed a stable monophyletic clade and a sister group to the clade comprising all Streptomyces species. Although there were several discrepancies in the details, considerable agreement was found between the results of rpoB analysis and those of numerical phenetic classification. This study demonstrates that analysis of rpoB can be used as an alternative genetic method in parallel to conventional taxonomic methods, including numerical phenetic and 16S rDNA analyses, for the phylogenetic analyses of the genera Streptomyces and Kitasatospora.
journal_name
Int J Syst Evol Microbiolauthors
Kim BJ,Kim CJ,Chun J,Koh YH,Lee SH,Hyun JW,Cha CY,Kook YHdoi
10.1099/ijs.0.02941-0keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
593-598issue
Pt 2eissn
1466-5026issn
1466-5034journal_volume
54pub_type
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journal_title:International journal of systematic and evolutionary microbiology
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journal_title:International journal of systematic and evolutionary microbiology
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journal_title:International journal of systematic and evolutionary microbiology
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