Abstract:
BACKGROUND:Characterization of seafood allergens is important to understand the immune response to these allergens. Moreover, a detailed comparison between atopic and nonatopic patients with adverse reactions to shrimp has never been reported. METHODS:Raw and boiled shrimp extracts were analyzed by immunoblotting using sera from 9 atopic and 7 nonatopic patients with a history of adverse reactions to shrimp, and 13 control subjects. Total IgE, specific IgE and skin prick tests (SPT) to shrimp were also investigated. RESULTS:The level of specific IgE to shrimp was higher in atopic patients than nonatopic patients (p<0.05). Symptoms, SPT results and major allergens involved were similar in atopic and nonatopic patients. The 16.5-kD protein had the highest frequency of IgE binding followed by the 40-kD protein in these patients. Other minor IgE-binding proteins were observed at the 20-, 22-, 54-, 72-, 129- and 140-kD regions. Patients who had binding to the 16.5-kD protein had either positive (25% raw/31% cooked) or negative (13% raw/cooked) CAP-FEIA-RAST, while patients who recognized the 40-kD protein all had positive (31% raw/19% cooked) CAP-FEIA-RAST. All control subjects had negative immunoblots for these two proteins. CONCLUSION:The 16.5-kD protein was the most frequent protein identified regardless of CAP-FEIA-RAST results, while the 40-kD protein was only present in patients with positive CAP-FEIA-RAST. Therefore, 16.5-kD protein may be an important allergen that is clinically relevant in both atopic and nonatopic patients with adverse reactions to shrimp even if it is not detected by the CAP-FEIA-RAST system.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Samson KT,Chen FH,Miura K,Odajima Y,Iikura Y,Naval Rivas M,Minoguchi K,Adachi Mdoi
10.1159/000076828keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
225-32issue
3eissn
1018-2438issn
1423-0097pii
76828journal_volume
133pub_type
杂志文章abstract::The last decade has seen the rapid development of research investigating the mechanisms of apoptosis in a variety of experimental systems. Among the multitude of changes observed in apoptotic cells, chromatin cleavage is considered a biochemical hallmark of apoptosis. Chromatin fragmentation is an enzymatic process wh...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000236778
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abstract::Asthmatic individuals in the general population appear to be susceptible to disease exacerbation during summertime 'smog' episodes (ambient air pollution containing other pollutants in addition to ozone). Although controlled exposure to ozone causes acute decrements in lung function, asthmatic subjects are only margin...
journal_title:International archives of allergy and immunology
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abstract::A quantitative determination of the inflammatory mediators was performed and correlated with complaints and the measurement of the inflammatory cells in nasal secretions of 18 seasonal allergic rhinitis patients (group 1) outside the pollen season and 40 symptomatic patients (group 2) with seasonal allergic rhinitis d...
journal_title:International archives of allergy and immunology
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abstract::It has been well documented that environmental factors such as antigen-presenting cells and related cytokines can affect the development of T-helper cells. The purpose of this study was to investigate the role of different antigen-presenting cells on T-cell development. Ovalbumin (OVA) combined with complete Freud's a...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237394
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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abstract::In studies designed to optimize the production and detection of anti-ovalbumin (anti-Oa) IgE in Ham/ICR mice, a range of doses of both Oa and pertussis toxin as the IgE adjuvant was explored. As determined by 48-hour passive cutaneous anaphylaxis (PCA) tests, the highest titre of anti-Oa IgE was obtained in a three-in...
journal_title:International archives of allergy and immunology
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abstract::Psoriasis (Ps) is a chronic, immune-mediated, skin inflammatory disease affecting up to 3% of the population worldwide. Different environmental triggers initiate this complex multifactorial syndrome. Many individuals affected by Ps (6-26%) develop inflammatory disease in other organs, often in the joints as in psorias...
journal_title:International archives of allergy and immunology
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abstract:BACKGROUND:Recombinant dog allergens, rCan f 1 and rCan f 2, and their antibodies are good tools for the characterization of dog allergens in order to develop modern therapeutic and preventive methods for dog allergy. METHODS:In this study, cDNA was synthesized from the mRNA of dog salivary glands and cloned into the ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000097498
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236602
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abstract:BACKGROUND:We have previously shown that the allergic sensitization to ovalbumin does not represent a superantigen-like immune response. In gene-targeted mice (ΔD-iD) with a single modified Diversity gene segment (D(H)) of the immunoglobulin heavy chain, enriched for charged amino acids, the asthma phenotype in a murin...
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pub_type: 杂志文章
doi:10.1159/000323527
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000328789
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abstract::Patients with atopic dermatitis (AD) are frequently colonized with Staphylococcus aureus strains secreting exotoxins such as the staphylococcal enterotoxin B (SEB) and A (SEA). Nonetheless the role of SEB and SEA in AD is yet unknown. We analyzed the responsiveness of peripheral blood mononuclear cells (PBMCs) and iso...
journal_title:International archives of allergy and immunology
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doi:10.1159/000236832
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pub_type: 杂志文章
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236168
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abstract::Antiphospholipid antibody syndrome (APS) is characterized by autoantibodies against negatively charged phospholipids in the serum, and clinically by multiple thromboses, thrombocytopenia, and recurrent fetal loss. The mechanism(s) by which the antibodies cause the clinical picture are not clear. We review the possible...
journal_title:International archives of allergy and immunology
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doi:10.1159/000236883
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abstract::Superoxide (O2-) and nitric oxide (NO) production by polymorphonuclear leukocyte (PMNs) and monocytes in patients with liver cirrhosis were evaluated. PMNs obtained from cirrhotic patients were less effective than those from controls in producing O2- after stimulation with opsonized zymosan, while they were more effec...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236975
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abstract::Peritoneal mast cells of Syrian hamsters release histamine to the action of concanavalin A (Con A) in dose-dependent fashion. The rate of release was very rapid in the first seconds of cell activation and completed in 60 s after the challenge. Morphological changes concomitant to the lectin treatment, followed by elec...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236113
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abstract::Preferential eosinophil accumulation is characteristic of airway inflammation in asthma. Although little is known about its mechanism, the effect of a sialyl Lewis X analog on airway eosinophilia was examined in a guinea-pig model of asthma. Guinea-pigs were sensitized by repeated inhalation of ovalbumin. After a sing...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237412
更新日期:1996-01-01 00:00:00
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journal_title:International archives of allergy and immunology
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