Abstract:
:Bone marrow-derived cells have demonstrated the ability to differentiate into multiple mesenchymal cell lineages. Here we tested whether smooth muscle (SM)-like tissues can be created in vivo with bone marrow stromal cells (BMSCs). Cultured canine BMSCs, which expressed SM cell-specific markers including SM alpha-actin and SM myosin heavy chain, were seeded on three-dimensional, biodegradable polymer scaffolds and implanted into peritoneal cavity of athymic mice. The cell-scaffold constructs retrieved 4 weeks after implantation formed three-dimensional tissues. Immunohistochemical analyses showed that the tissue reconstructs expressed SM alpha-actin and SM myosin heavy chain. Masson's trichrome staining showed the presence of significant amounts of collagen in the tissue reconstructs. Cells labeled with a fluorescent tracer prior to implantation were still present in the tissue reconstructs 4 weeks after implantation. Non-seeded scaffolds (control groups) retrieved 4 weeks after implantation did not exhibit extensive tissue formation. This study demonstrates the potential of BMSCs as an alternative cell source for tissue engineering of SM.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Cho SW,Kim IK,Lim SH,Kim DI,Kang SW,Kim SH,Kim YH,Lee EY,Choi CY,Kim BSdoi
10.1016/j.biomaterials.2003.09.068keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
2979-86issue
15eissn
0142-9612issn
1878-5905pii
S014296120300810Xjournal_volume
25pub_type
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