Osteoblast response to PLGA tissue engineering scaffolds with PEO modified surface chemistries and demonstration of patterned cell response.

Abstract:

:Because tissues are characterized by a well-defined three-dimensional arrangement of cells, tissue engineering scaffolds that facilitate the organization and differentiation of new tissue will have improved performance in comparison to scaffolds that only provide surfaces for cell attachment and growth. We hypothesize that instructions for cells can be incorporated into tissue engineering scaffolds by patterning the scaffold's architecture and surface chemistry. Our goals for the presented work were to collect data about cell response to three-dimensional, porous scaffolds with uniformly modified surfaces chemistries, and to demonstrate patterning of cell response by patterning surface chemistry. Our system was osteoblast response to poly(l-lactide-co-glycolide) scaffolds modified with poly(ethylene oxide) (PEO). Scaffolds were fabricated using the Three-Dimensional Printing (3DP) process which has control over scaffolds properties to a resolution of approximately 100 microm in all three dimensions. At higher PEO concentrations, adhesion, growth rates, and migration of rat osteoblasts were reduced; alkaline phosphate activity was increased, and cells were less spread and had microvilli. Patterned regions of low and high cell adhesion were demonstrated on scaffolds fabricated with 1 mm thick stripes of PEO and non-PEO regions.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Koegler WS,Griffith LG

doi

10.1016/j.biomaterials.2003.09.064

keywords:

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

2819-30

issue

14

eissn

0142-9612

issn

1878-5905

pii

S0142961203008068

journal_volume

25

pub_type

杂志文章
  • Nanodiamonds coupled with plant bioactive metabolites: a nanotech approach for cancer therapy.

    abstract::Nanodiamond application in biotechnological and medical fields is nowadays in continuous progress. In fact, biocompatibility, reduced dimensions and high surface chemical interaction are specific features that make nanodiamonds perfect intracellular carriers of bioactive compounds. By confocal microscopy, we confirmed...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2014.10.057

    authors: Gismondi A,Reina G,Orlanducci S,Mizzoni F,Gay S,Terranova ML,Canini A

    更新日期:2015-01-01 00:00:00

  • General solution of the diffusion equation: application to formaldehyde sterilization and desorption of polymers.

    abstract::A general solution of the diffusion equation for gas in polymers is developed by a numerical method. This solution allows diffusion to be calculated in all cases, by gas desorption after an incomplete adsorption, which is not the case for analytical solutions. When the analytical solutions are valid, both types of sol...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/0142-9612(87)90004-4

    authors: Hennebert P

    更新日期:1987-09-01 00:00:00

  • Anticomplementary activity of dextran derivatives.

    abstract::Substitution with carboxylic and benzylamine sulphonated groups conferred on dextran both antithrombic activity and the capacity to inhibit formation of the amplification C3 convertase of complement. In dextrans substituted with carboxylic groups (greater than 40%), a high content of sulphonate (greater than 10%) resu...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/0142-9612(85)90040-7

    authors: Mauzac M,Maillet F,Jozefonvicz J,Kazatchkine MD

    更新日期:1985-01-01 00:00:00

  • The effects of heparin releasing hydrogels on vascular smooth muscle cell phenotype.

    abstract::Poly(ethylene glycol) diacrylate (PEGDA) hydrogel scaffolds were engineered to promote contractile smooth muscle cell (SMC) phenotype via controlled release of heparin. The scaffold design was evaluated by quantifying the effects of free heparin on SMC phenotype, engineering hydrogels to provide controlled release of ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2009.08.004

    authors: Beamish JA,Geyer LC,Haq-Siddiqi NA,Kottke-Marchant K,Marchant RE

    更新日期:2009-10-01 00:00:00

  • Analysis and evaluation of a biomedical polycarbonate urethane tested in an in vitro study and an ovine arthroplasty model. Part I: materials selection and evaluation.

    abstract::The polyurethane elastomer (PU) Corethane 80A (Corvita) is being considered as the acetabular bearing material in a novel total replacement hip joint. The biostability of Corethane 80A was investigated in vitro (this work) and in vivo (reported separately) in a fully functioning ovine total hip arthroplasty (THA) mode...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2004.02.065

    authors: Khan I,Smith N,Jones E,Finch DS,Cameron RE

    更新日期:2005-02-01 00:00:00

  • Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineering.

    abstract::Approximately 285 million people worldwide suffer from diabetes, with insulin supplementation as the most common treatment measure. Regenerative medicine approaches such as a bioengineered pancreas has been proposed as potential therapeutic alternatives. A bioengineered pancreas will benefit from the development of a ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2013.05.066

    authors: Goh SK,Bertera S,Olsen P,Candiello JE,Halfter W,Uechi G,Balasubramani M,Johnson SA,Sicari BM,Kollar E,Badylak SF,Banerjee I

    更新日期:2013-09-01 00:00:00

  • Blood-brain barrier dysfunction induced by silica NPs in vitro and in vivo: Involvement of oxidative stress and Rho-kinase/JNK signaling pathways.

    abstract::Silica nanoparticles (SiO2-NPs) has been extensively exploited in biomedical fields and mostly designed to enter the circulatory system, however, few studies focused on the potential adverse effects of SiO2-NPs exposure on the blood-brain barrier (BBB) that serves as a critical barrier between the central nervous syst...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2017.01.006

    authors: Liu X,Sui B,Sun J

    更新日期:2017-03-01 00:00:00

  • Strong biofilm production, antibiotic multi-resistance and high gelE expression in epidemic clones of Enterococcus faecalis from orthopaedic implant infections.

    abstract::Enterococcus faecalis is an opportunistic pathogen, which today represents one of the leading aetiologic agents of nosocomial infections and, increasingly, of implant infections. Here, in a collection of 43 E. faecalis isolated from implant orthopaedic infections, virulence-related phenotypes (biofilm and gelatinase p...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2007.10.008

    authors: Arciola CR,Baldassarri L,Campoccia D,Creti R,Pirini V,Huebner J,Montanaro L

    更新日期:2008-02-01 00:00:00

  • Induction of protein oxidation by cobalt and chromium ions in human U937 macrophages.

    abstract::Metal particles and ions from hip prostheses have the potential to induce the production of reactive oxygen species (ROS), making them prime suspects for disturbing the cellular balance of oxidants/antioxidants (redox state of the cell). To better understand the cellular effect of metal ions from metal-on-metal prosth...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2004.11.019

    authors: Petit A,Mwale F,Tkaczyk C,Antoniou J,Zukor DJ,Huk OL

    更新日期:2005-07-01 00:00:00

  • Engineering PEG-based hydrogels to foster efficient endothelial network formation in free-swelling and confined microenvironments.

    abstract::In vitro tissue engineered models are poised to have significant impact on disease modeling and preclinical drug development. Reliable methods to induce microvascular networks in such microphysiological systems are needed to improve the size and physiological function of these models. By systematically engineering sev...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2020.119921

    authors: Brown A,He H,Trumper E,Valdez J,Hammond P,Griffith LG

    更新日期:2020-06-01 00:00:00

  • The effects of a functionally-graded scaffold and bone marrow-derived mononuclear cells on steroid-induced femoral head osteonecrosis.

    abstract::Osteonecrosis of the femoral head (ONFH) is a debilitating disease that may progress to femoral head collapse and subsequently, degenerative arthritis. Although injection of bone marrow-derived mononuclear cells (BMMCs) is often performed with core decompression (CD) in the early stage of ONFH, these treatments are no...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2018.09.030

    authors: Maruyama M,Nabeshima A,Pan CC,Behn AW,Thio T,Lin T,Pajarinen J,Kawai T,Takagi M,Goodman SB,Yang YP

    更新日期:2018-12-01 00:00:00

  • Post-nuclear gene delivery events for transgene expression by biocleavable polyrotaxanes.

    abstract::A quantitative comparison between nuclear DNA release from carriers and their transfection activity would be highly useful for improving the effectiveness of non-viral gene vectors. We previously reported that, for condensed DNA particles, a close relationship exists between the efficiency of DNA release and transfect...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2012.01.049

    authors: Yamada Y,Nomura T,Harashima H,Yamashita A,Yui N

    更新日期:2012-05-01 00:00:00

  • Hyaluronic acid hydrogel in the treatment of osteoarthritis.

    abstract::In order to overcome the problem of rapid clearance of the polysaccharide hyaluronic acid (Hyal) in the treatment of osteoarthritis (OA), a 50% cross-linked Hyal hydrogel (Hyal 50%) was synthesised. The 50% refers to the amount of COOH groups of the polysaccharide involved in the cross-linking reaction. i.e. 50% of th...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(02)00194-1

    authors: Barbucci R,Lamponi S,Borzacchiello A,Ambrosio L,Fini M,Torricelli P,Giardino R

    更新日期:2002-12-01 00:00:00

  • Effect of water-soluble phospholipid polymers conjugated with papain on the enzymatic stability.

    abstract::To maintain enzymatic activity during long-term storage by conjugation with water-soluble 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers (PMPC-COOH) having various molecular weights with a carboxylic group on the terminal, such compounds were synthesized as a polymer modifier using a photoinduced living radic...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(03)00474-5

    authors: Miyamoto D,Watanabe J,Ishihara K

    更新日期:2004-01-01 00:00:00

  • Localized inhibition of platelets and platelet derived growth factor by a matrix targeted glycan mimetic significantly attenuates liver fibrosis.

    abstract::New therapeutic strategies are needed for the growing unmet clinical needs in liver disease and fibrosis. Platelet activation and PDGF activity are recognized as important therapeutic targets; however, no therapeutic approach has yet addressed these two upstream drivers of liver fibrosis. We therefore designed a matri...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2020.120538

    authors: Kabra H,Walimbe T,Stuart K,Indey C,Jalgaonkar S,Ikwa E,Skurnac T,Chen J,Woolley A,Snead NM,Bachtell N,Leeming DJ,Karsdal M,Prestwich G,Panitch A,Paderi J

    更新日期:2020-11-17 00:00:00

  • Tear and rupture of elastomeric dental impression materials.

    abstract::The curing time dependence of the tear energy, tensile strength and ultimate extension ratio of a range of characterized elastomeric impression materials was investigated. This variation of tensile strength and ultimate extension ratio was correlated with the development of the network structure. The tear energy showe...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/0142-9612(84)90072-3

    authors: Cook WD,Liem F,Russo P,Scheiner M,Simkiss G,Woodruff P

    更新日期:1984-09-01 00:00:00

  • Degradation of poly[bis(glycine ethyl ester)phosphazene] in aqueous media.

    abstract::The degradation of poly[bis(glycine ethyl ester)phosphazene] in aqueous media was studied by following the appearance of some of its expected by-products in solution. Disc-shaped polymer slabs of 12 mm diameter and 1.2 mm thickness were prepared by compression moulding, followed by incubation in aqueous media under ca...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/0142-9612(93)90235-t

    authors: Ruiz EM,Ramírez CA,Aponte MA,Barbosa-Cánovas GV

    更新日期:1993-06-01 00:00:00

  • Electrical stimulation and piezoelectric biomaterials for bone tissue engineering applications.

    abstract::Bioelectrical phenomenon in natural bone has been well recognized for its role in bone development and fracture healing. For example, the piezoelectricity induced modulation in cellular functionality assists in the repair and regeneration of bone tissue. Against this backdrop, we review here the origin of dielectric a...

    journal_title:Biomaterials

    pub_type: 杂志文章,评审

    doi:10.1016/j.biomaterials.2020.120280

    authors: Khare D,Basu B,Dubey AK

    更新日期:2020-11-01 00:00:00

  • Preparation of three-dimensional interconnected macroporous cellulosic hydrogels for soft tissue engineering.

    abstract::This work exploits the thermal responsive phase behavior of hydroxypropylcellulose to produce 3D interconnected macroporous hydrogels in aqueous environment. Hydroxypropylcellulose was modified with allyl isocyanate, and their temperature mediated phase behavior was studied as a function of degree of modification (DS)...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2010.07.059

    authors: Yue Z,Wen F,Gao S,Ang MY,Pallathadka PK,Liu L,Yu H

    更新日期:2010-11-01 00:00:00

  • Prion inhibition with multivalent PrPSc binding compounds.

    abstract::Quinacrine and related heterocyclic compounds have antiprion activity. Since the infectious pathogen of prion diseases is composed of multimeric PrP(Sc) assemblies, we hypothesized that this antiprion property could be enhanced by attaching multiple quinacrine-derived chloroquinoline or acridine moieties to a scaffold...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2012.06.004

    authors: Mays CE,Joy S,Li L,Yu L,Genovesi S,West FG,Westaway D

    更新日期:2012-10-01 00:00:00

  • Mechanism of thrombin inhibition by antithrombin and heparin cofactor II in the presence of heparin.

    abstract::The kinetics of thrombin inhibition by antithrombin (AT) and heparin cofactor II (HC II) were analysed as a function of the heparin concentration, from 10(-9) to 10(-4) M. The initial concentrations of inhibitor (l) and thrombin (E) were set at equimolar levels (CI = CE = 10(-8) M). The experimental data indicate that...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(96)00125-1

    authors: Maaroufi RM,Jozefowicz M,Tapon-Bretaudière J,Fischer AM

    更新日期:1997-02-01 00:00:00

  • Porous collagen-apatite nanocomposite foams as bone regeneration scaffolds.

    abstract::We have created a porous bioresorbable nanocomposite bone scaffold that chemically, structurally and mechanically matched natural bone so that it could be recognized and remodeled by natural bone. Containing collagen fibers and synthetic apatite nanocrystals, our scaffold has high strength for supporting the surroundi...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2008.07.030

    authors: Pek YS,Gao S,Arshad MS,Leck KJ,Ying JY

    更新日期:2008-11-01 00:00:00

  • Microspheres of hydroxyapatite/reconstituted collagen as supports for osteoblast cell growth.

    abstract::Microspheres comprised of particulate hydroxyapatites dispersed in fibrous collagen matrices were prepared. The procedure involved the droplet formation of hydroxyapatite/collagen mixture emulsified in olive oil, followed by the reconstitution of collagen in the presence of hydroxyapatite particles at 37 degrees C. Va...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(99)00095-2

    authors: Hsu FY,Chueh SC,Wang YJ

    更新日期:1999-10-01 00:00:00

  • Self-protecting core-shell magnetic nanoparticles for targeted, traceable, long half-life delivery of BCNU to gliomas.

    abstract::The successful delivery of anti-cancer drugs relies on the simultaneous capability to actively target a specific location, a sufficient lifetime in the active form in the circulation, and traceability and quantification of drug distribution via in vivo medical imaging. Herein, a highly magnetic nanocarrier (HMNC) comp...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2011.05.047

    authors: Yang HW,Hua MY,Liu HL,Huang CY,Tsai RY,Lu YJ,Chen JY,Tang HJ,Hsien HY,Chang YS,Yen TC,Chen PY,Wei KC

    更新日期:2011-09-01 00:00:00

  • The nano-morphological relationships between apatite crystals and collagen fibrils in ivory dentine.

    abstract::In this work, analytical transmission electron microscopy (TEM) was used to study the nanostructure of mineralised ivory dentine, in order to gain a clearer understanding of the relationship between the organic (collagen fibrils) and inorganic (calcium phosphate apatite crystals) components. Thin sections prepared by ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2010.03.025

    authors: Jantou-Morris V,Horton MA,McComb DW

    更新日期:2010-07-01 00:00:00

  • Assessment of in vivo degradation profiles of hyaluronic acid hydrogels using temporal evolution of chemical exchange saturation transfer (CEST) MRI.

    abstract::Hyaluronic acid (HA) hydrogels have found a wide range of applications in biomedicine: regenerative medicine to drug delivery applications. In vivo quantitative assessment of these hydrogels using magnetic resonance imaging (MRI) provides an effective, accurate, safe, and non-invasive translational approach to assess ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2018.05.037

    authors: Shazeeb MS,Corazzini R,Konowicz PA,Fogle R,Bangari DS,Johnson J,Ying X,Dhal PK

    更新日期:2018-09-01 00:00:00

  • Graphene quantum dots conjugated neuroprotective peptide improve learning and memory capability.

    abstract::Alzheimer disease (AD) is a neurodegenerative disorder and the most common form of dementia. Histopathologically is characterized by the presence extracellular neuritic plaques and with a large number of neurons lost. In this paper, we design a new nanomaterial, graphene quantum dots (GQDs) conjugated neuroprotective ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2016.08.021

    authors: Xiao S,Zhou D,Luan P,Gu B,Feng L,Fan S,Liao W,Fang W,Yang L,Tao E,Guo R,Liu J

    更新日期:2016-11-01 00:00:00

  • Effects of topography and composition of titanium surface oxides on osteoblast responses.

    abstract::To investigate the roles of composition and characteristics of titanium surface oxides in cellular behaviour of osteoblasts, the surface oxides of titanium were modified in composition and topography by anodic oxidation in two kinds of electrolytes, (a) 0.2 M H(3)PO(4), and (b) 0.03 M calcium glycerophosphate (Ca-GP) ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2003.11.011

    authors: Zhu X,Chen J,Scheideler L,Reichl R,Geis-Gerstorfer J

    更新日期:2004-08-01 00:00:00

  • Structural characterization of room-temperature synthesized nano-sized beta-tricalcium phosphate.

    abstract::A simple route for synthesizing nano-sized beta-tricalcium phosphate (beta-TCP) at room temperature has been developed in methanol solvent. The phase evolution from CaHPO4, intermediate amorphous calcium phosphate (ACP) phases (including ACP1 and ACP2 with different structures) to final beta-TCP with increasing aging ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2003.10.046

    authors: Bow JS,Liou SC,Chen SY

    更新日期:2004-07-01 00:00:00

  • Formation of surface reaction products on bioactive glass and their effects on the expression of the osteoblastic phenotype and the deposition of mineralized extracellular matrix.

    abstract::The objective of the study was to examine the effect of alkali ion release, pH control and buffer capacity on the expression of the osteoblastic phenotype. In addition we determined the importance of modifications of the surface of porous bioactive glass (BG) on the activity of rat calvaria osteoblasts in vitro. We fo...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(96)00059-2

    authors: el-Ghannam A,Ducheyne P,Shapiro IM

    更新日期:1997-02-01 00:00:00