Abstract:
:The phytohemagglutinin (PHA)-stimulated human lymphocytes demonstrated trace or no activity (dihydro) folate acid reductase using three methods including a radioassay, but demonstrated ample activity of thymidylate synthetase. This was true regardless of the day of harvest, (first through seventh) of the stimulated lymphocyte. The lymphocyte extracts revealed no inhibitor to the reductase enzyme when these extracts were added before the liver extracts to the assay system. When methotrexate (MTX) was added to the culture media of the lymphocytes, there was, as expected, an increase in the synthetase activity, but the expected rise in the reductase activity did not occur, it remained nil. On the other hand, MTX did influence the incorporation of nucleosides by the stimulated lymphocytes in a fashion similar to its action on the incorporation of the same nucleosides by other cells.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Haurani FI,Kardinal CG,Biermann WAdoi
10.1002/jcp.1040950107keywords:
subject
Has Abstractpub_date
1978-04-01 00:00:00pages
49-55issue
1eissn
0021-9541issn
1097-4652journal_volume
95pub_type
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