Abstract:
:It is generally accepted that tuberculosis results from a single infection with a single Mycobacterium tuberculosis strain. Such infections are thought to confer protective immunity against exogenous reinfection. In this study, a novel polymerase chain reaction method was developed to specifically identify M. tuberculosis strains belonging to the Beijing and non-Beijing evolutionary lineages in sputum specimens collected from tuberculosis patients resident in an epidemiologic field site in Cape Town, South Africa. The sensitivity and specificity of the polymerase chain reaction-based strain classification method were 100% (95% confidence interval, 85-100%) when compared with DNA fingerprinting and spacer oligotyping (spoligotyping). Application of this method showed that 19% of all patients were simultaneously infected with Beijing and non-Beijing strains, and 57% of patients infected with a Beijing strain were also infected with a non-Beijing strain. Multiple infections were more frequent in retreatment cases (23%) as compared with new cases (17%), but were not associated with sex, age, or smear grading. These results suggest that multiple infections are frequent, implying high reinfection rates and the absence of efficient protective immunity conferred by the initial infection. This finding could influence our understanding of the epidemiology of disease in high-incidence regions and our understanding for vaccine development.
journal_name
Am J Respir Crit Care Medauthors
Warren RM,Victor TC,Streicher EM,Richardson M,Beyers N,Gey van Pittius NC,van Helden PDdoi
10.1164/rccm.200305-714OCkeywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
610-4issue
5eissn
1073-449Xissn
1535-4970pii
200305-714OCjournal_volume
169pub_type
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journal_title:American journal of respiratory and critical care medicine
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journal_title:American journal of respiratory and critical care medicine
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journal_title:American journal of respiratory and critical care medicine
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journal_title:American journal of respiratory and critical care medicine
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pub_type: 杂志文章
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更新日期:2005-06-15 00:00:00
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journal_title:American journal of respiratory and critical care medicine
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更新日期:2017-12-15 00:00:00
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journal_title:American journal of respiratory and critical care medicine
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