Abstract:
:In this work we present a probabilistic model to estimate summaries of Affymetrix GeneChip probe level data. Comparisons with two different models were made both on a publicly available dataset and on a study performed in our laboratory, showing that our model performs better for consistency of fold change.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Milo M,Fazeli A,Niranjan M,Lawrence NDdoi
10.1042/bst0311510keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
1510-2issue
Pt 6eissn
0300-5127issn
1470-8752journal_volume
31pub_type
杂志文章abstract::Protein ubiquitination and protein phosphorylation are two fundamental regulatory post-translational modifications controlling intracellular signalling events. However, the ubiquitin system is vastly more complex compared with phosphorylation. This is due to the ability of ubiquitin to form polymers, i.e. ubiquitin ch...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0370937
更新日期:2009-10-01 00:00:00
abstract::After years of setbacks, the perspective of neuroprotective stroke therapy has revived in light of recent study results. We outline in this review how a neuroprotective candidate drug should be developed, beginning with a thorough preclinical evaluation according to the STAIR (Stroke Therapy Academic Industry Roundtab...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0341271
更新日期:2006-12-01 00:00:00
abstract::TLR8 (Toll-like receptor 8) is activated by ssRNAs (single-stranded RNAs) and synthetic imidazoquinoline compounds that resemble purines and have immunostimulatory activity. TLR8 agonists are particularly effective at inducing Th1-polarizing responses from human monocytes and myeloid dendritic cells, with the magnitud...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0351485
更新日期:2007-12-01 00:00:00
abstract::Luteinizing hormone (LH) interacts with its plasma membrane receptor to activate the formation of cyclic AMP via the regulatory GTP binding protein (Gs). This is followed by a desensitization of that same hormonal response which is caused by an uncoupling of the LH receptor from Gs. The coupling between Gs and the ade...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0170633
更新日期:1989-08-01 00:00:00
abstract::The SERCA (sarcoplasmic/endoplasmic reticulum Ca2+-ATPase) is probably the most extensively studied membrane protein transporter. There is a vast array of diverse inhibitors for the Ca2+ pump, and many have proved significant in helping to elucidate both the mechanism of transport and gaining conformational structures...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0390789
更新日期:2011-06-01 00:00:00
abstract::Aberrant activation of the PI3K pathway is one of the commonest oncogenic events in human cancer. AKT is a key mediator of PI3K oncogenic function, and thus has been intensely pursued as a therapeutic target. Multiple AKT inhibitors, broadly classified as either ATP-competitive or allosteric, are currently in various ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20190777
更新日期:2020-06-30 00:00:00
abstract::The role of Hsp70 (heat-shock protein 70) chaperones in assisting protein-folding processes relies on their ability to associate with short peptide stretches of protein substrates in a transient and ATP-controlled manner. In the present study, we review the molecular details of the mechanism behind substrate recogniti...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0320617
更新日期:2004-08-01 00:00:00
abstract::Evidence indicates that leucine-rich repeat kinase 2 (LRRK2) controls multiple processes in neurons and glia cells. Deregulated LRRK2 activity due to gene mutation represents the most common cause of autosomal dominant Parkinson's disease (PD). Protein kinase A (PKA)-mediated signaling is a key regulator of brain func...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20160396
更新日期:2017-02-08 00:00:00
abstract::Most research strategies for cartilage tissue engineering use extended culture with complex media loaded with costly GFs (growth factors) to drive tissue assembly and yet they result in the production of cartilage with inferior mechanical and structural properties compared with the natural tissue. Recent evidence sugg...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20140031
更新日期:2014-06-01 00:00:00
abstract::Colicins are proteins produced by some strains of Escherichia coli to kill competitors belonging to the same species. Among them, ColM (colicin M) is the only one that blocks the biosynthesis of peptidoglycan, a specific bacterial cell-wall polymer essential for cell integrity. ColM acts in the periplasm by hydrolysin...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20120189
更新日期:2012-12-01 00:00:00
abstract::Along with the development of several large-scale methods such as mass spectrometry or micro arrays, genome wide models became not only a possibility but an obvious tool for theoretical biologists to integrate and analyse complex biological data. Nevertheless, incorporating the dynamics of photosynthesis remains one o...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20150136
更新日期:2015-12-01 00:00:00
abstract::Oral and maxillofacial surgery is often challenging due to defective bone healing owing to the microbial environment of the oral cavity, the additional involvement of teeth and esthetic concerns. Insufficient bone volume as a consequence of aging and some oral and maxillofacial surgical procedures, such as tumor resec...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20200376
更新日期:2020-06-30 00:00:00
abstract::Colorectal cancer is the third most common cancer in developed countries such as the U.K., but incidence rates around the world vary approx. 20-fold. Diet is thought to be a key factor determining risk: red and processed meat, but not white meat or fish, are associated with an increased risk of colorectal cancer. The ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0351355
更新日期:2007-11-01 00:00:00
abstract::The giant cytoskeletal kinases of the titin-like family are emerging as key mediators of stretch-sensing in muscle. It is thought that their elastic conformational deformation during muscle function regulates both their catalysis and the recruitment of regulatory proteins to signalosomes that assemble in their vicinit...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20130085
更新日期:2013-08-01 00:00:00
abstract::LRRK2 mutation is the most common inherited, autosomal dominant cause of Parkinson's disease (PD) and has also been observed in sporadic cases. Most mutations result in increased LRRK2 kinase activity. LRRK2 is highly expressed in brain regions that receive dense, convergent innervation by dopaminergic and glutamaterg...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20180468
更新日期:2018-12-17 00:00:00
abstract::Crystal structure data for Escherichia coli beta-ketoacyl synthase (KAS) I with C(10) and C(12) fatty acid substrates bound in conjunction with results from mutagenizing residues in the active site leads to a model for catalysis. Differences from and similarities to the other Claisen enzymes carrying out decarboxylati...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/0300-5127:0280601
更新日期:2000-12-01 00:00:00
abstract::Trim25 is a member of the tripartite motif family of E3 ubiquitin ligases. It plays major roles in innate immunity and defence against viral infection, control of cell proliferation and migration of cancer cells. Recent work identified Trim25 as being able to bind to RNA and to regulate Lin28a-mediated uridylation of ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20160077
更新日期:2016-08-15 00:00:00
abstract::The PDC (pyruvate dehydrogenase complex) plays a central role in the maintenance of glucose homoeostasis in mammals. The carbon flux through the PDC is meticulously controlled by elaborate mechanisms involving post-translational (short-term) phosphorylation/dephosphorylation and transcriptional (long-term) controls. T...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20060217
更新日期:2006-04-01 00:00:00
abstract::Large dense-core vesicles (LDCVs) were labelled in cultured bovine adrenal chromaffin cells expressing fluorescent chimaeric 'cargo' proteins that were targeted to these secretory vesicles. When the cells were stimulated with nicotine 48 h after transduction, the fractional loss of fluorescent LDCVs was much greater t...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0310851
更新日期:2003-08-01 00:00:00
abstract::Identified five decades ago amongst the most abundant cellular RNAs, small nucleolar RNAs (snoRNAs) were initially described as serving as guides for the methylation and pseudouridylation of ribosomal RNA through direct base pairing. In recent years, however, increasingly powerful high-throughput genomic approaches an...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20191046
更新日期:2020-04-29 00:00:00
abstract::The adhesion of cells to the extracellular matrix is a dynamic process, mediated by a series of cell-surface and matrix-associated molecules that interact with each other in a spatially and temporally regulated manner. These interactions play a major role in tissue formation, cellular migration and the induction of ad...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0320416
更新日期:2004-06-01 00:00:00
abstract::The development of natural tissues, organs and bodies depends on mechanisms of patterning and of morphogenesis, typically (but not invariably) in that order, and often several times at different final scales. Using synthetic biology to engineer patterning and morphogenesis will both enhance our basic understanding of ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20200013
更新日期:2020-06-30 00:00:00
abstract::The collagens of vertebrates may be divided into three groups according to chain size and whether or not the helical domains are continuous. Present evidence suggests that, at least within one of these groups, similarity between collagens is reflected in the organization of the genes that encode them. Early evidence s...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0160661
更新日期:1988-10-01 00:00:00
abstract::BK (large conductance calcium- and voltage-activated potassium) channels are important determinants of physiological control in the nervous, endocrine and vascular systems with channel dysfunction associated with major disorders ranging from epilepsy to hypertension and obesity. Thus the mechanisms that control channe...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20120226
更新日期:2013-02-01 00:00:00
abstract::Peptide-bond formation is the enzymatic activity of the ribosome. The catalytic site is made up of ribosomal RNA, indicating that the ribosome is a ribozyme. This review summarizes the recent progress in understanding the mechanism of peptide bond formation. The results of biochemical and kinetic experiments, mutagene...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0330493
更新日期:2005-06-01 00:00:00
abstract::Muscles are responsible for generating the forces required for the movement of multicellular organisms. Microscopically, these forces arise as a consequence of motor proteins (myosin) pulling and sliding along actin filaments. Current knowledge states that the molecular forces between actin and myosin are linear in na...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0320694
更新日期:2004-11-01 00:00:00
abstract::GRIF-1 [GABA(A) (gamma-aminobutyric acid(A)) receptor interacting factor-1] is a member of a coiled-coil family of proteins thought to function as adaptors in the anterograde trafficking of organelles utilizing the kinesin-1 motor proteins to synapses. To study in more detail the molecular interaction between GRIF-1 a...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0340048
更新日期:2006-02-01 00:00:00
abstract::There is increasing appreciation among researchers and clinicians of the value of investigating biology and pathobiology at the level of cellular kinase (kinome) activity. Kinome analysis provides valuable opportunity to gain insights into complex biology (including disease pathology), identify biomarkers of critical ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20160163
更新日期:2017-02-08 00:00:00
abstract::Yeast cells rely on the SPS-sensing pathway to respond to extracellular amino acids. This nutrient-induced signal transduction pathway regulates gene expression by controlling the activity of two redundant transcription factors: Stp1 and Stp2. These factors are synthesized as latent cytoplasmic proteins with N-termina...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0370242
更新日期:2009-02-01 00:00:00
abstract::The network of the ER (endoplasmic reticulum) is set up by cytoskeletal control of the movement and remodelling of polygonal rings of tubules, bundles of tubules and cisternal regions. We have developed a new image analysis tool, persistency mapping, to understand the framework upon which the plant ER remodels. With t...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0380747
更新日期:2010-06-01 00:00:00