Abstract:
:RNase A (1 mM) was incubated with glucose (0.4 M) at 37 degrees C for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the major sites of N epsilon-(carboxymethyl)lysine formation.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Cotham WE,Hinton DJ,Metz TO,Brock JW,Thorpe SR,Baynes JW,Ames JMdoi
10.1042/bst0311426keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
1426-7issue
Pt 6eissn
0300-5127issn
1470-8752journal_volume
31pub_type
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