Abstract:
:A digoxigenin-labeled antisense 42-mer oligonucleotide was used for the localization of the dopamine D2 receptor mRNA in the rat brain. The digoxigenin label was identified with alkaline phosphatase conjugated sheep-anti-digoxigenin. In good analogy with the known terminal fields of the dopaminergic system, various nuclei throughout the brain were labeled. Positive in situ hybridization signals were also found in dopamine cell groups of the substantia nigra and ventral tegmental area and in regions where a dopaminergic innervation is controversial, like the cerebellar cortex and the hippocampus. The non-radioactive in situ hybridization procedure described, shows the localization of the dopamine D2 receptor mRNA with a very high contrast and an optimal histological resolution.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Brouwer N,Van Dijken H,Ruiters MH,Van Willigen JD,Ter Horst GJdoi
10.1016/0304-3940(92)90378-kkeywords:
subject
Has Abstractpub_date
1992-08-17 00:00:00pages
223-7issue
2eissn
0304-3940issn
1872-7972pii
0304-3940(92)90378-Kjournal_volume
142pub_type
杂志文章abstract::Excitatory amino acid (EAA) receptor-mediated events have recently been implicated in dopaminergic mechanisms of neurotoxicity. 2,4,5-Trihydroxyphenylalanine (6-hydroxy-DOPA, TOPA), the ortho-hydroxylated derivative of the dopamine precursor 2,4-dihydroxyphenylalanine (L-DOPA), has recently been reported to have neuro...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(91)90432-s
更新日期:1991-10-28 00:00:00
abstract::To assess the role of nasal/tracheal (N/T) breathing in the respiratory patterning of the olfactory bulb (OB) neurons, the activity of 21 units was recorded in 6 anesthetized rats set with a cannula enabling reversible tracheotomy: the rats could inhalate either through nasal pathways or through trachea directly. Shif...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90457-k
更新日期:1990-07-31 00:00:00
abstract::Although synaptically released, vesicular Zn(2+) has been proposed to play a neuromodulatory or neuronal signaling role at the mossy fiber-CA3 synapse, Zn(2+) release remains controversial, especially when detected using fluorescent imaging. In the present study, we investigated synaptically released Zn(2+) at the mos...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.04.047
更新日期:2007-05-29 00:00:00
abstract::The present study was performed to explore the effect of green tea polyphenols on the intracellular Aβ (iAβ)-induced toxicity to cultured rat primary prefrontal cortical neurons. Administration of 100nM, 1μM or 10μM of green tea polyphenols significantly inhibited the iAβ-induced toxicity to cultured rat primary prefr...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2012.02.029
更新日期:2012-04-04 00:00:00
abstract::L-Glutamate binding to cockroach central nervous system (CNS) membranes is unaffected by freeze-drying and subsequent rehydration. The dissociation constant is similarly unaltered by irradiation at a dose of 3 Mrad which affects only the number of biologically active binding sites. A molecular weight estimate for this...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90686-1
更新日期:1989-05-22 00:00:00
abstract::Neuraxial analgesia is often provided using a mixture of local anesthetics and opioids. This combination of agents provides better pain relief and is generally associated with fewer side effects than when either drug is given alone. Local anesthetics have been shown to alter signaling of other G protein-coupled recept...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.03.041
更新日期:2007-05-18 00:00:00
abstract::Drug addiction is a process that transits from recreative and regular drug use into compulsive drug use. The two patterns of drug use, controlled drug intake and escalated drug intake, represent different stages in the development of drug addiction; and escalation of drug use is a hallmark of addiction. Accumulating s...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2015.11.016
更新日期:2016-01-12 00:00:00
abstract::To study the central effects of adenosine on hypoxia, the influence of treatment by the A1 receptor agonist cyclohexyladenosine (1 microM) or by the antagonist theophylline (10 microM) was tested on cell damage in a model of neuronal culture. Whereas theophylline enhanced cell injury induced by 8 h hypoxia, cyclohexyl...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)91092-8
更新日期:1994-07-04 00:00:00
abstract::Pain is the most characteristic feature of sickle cell disease (SCD). Patients with SCD live with unpredictable, recurrent episodes of acute painful crisis, as well as chronic unremitting pain throughout their lifetime. While most of the research and medical efforts have focused on treating vaso-occlusion crisis and a...
journal_title:Neuroscience letters
pub_type: 杂志文章,评审
doi:10.1016/j.neulet.2019.04.055
更新日期:2019-07-27 00:00:00
abstract::Early onset generalized dystonia is a dominantly inherited movement disorder caused by neuronal dysfunction without an apparent loss of neurons. The same single mutation (GAG deletion) causes most cases and results in loss of a glutamic acid (E) in the carboxy terminal region of torsinA (Delta302/303). To model the ne...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.10.098
更新日期:2006-03-13 00:00:00
abstract::Calcitonin gene-related peptide (CGRP) is expressed in and defines a subset of motor neuron pools in the lumbar spinal cord of the chick embryo. The onset of CGRP expression in individual pools coincides with both the period of the initial innervation of the leg and the beginning of naturally occurring cell death in t...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90488-s
更新日期:1992-04-13 00:00:00
abstract::Limited work has been done to investigate the molecular mechanisms behind the process of demyelination and remyelination that occurs in response to chronic nerve compression (CNC) injury. In the present study, we investigated the expression of the transcription factors krox-20 and c-jun, positive and negative regulato...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.09.014
更新日期:2009-11-13 00:00:00
abstract::The isthmo-optic system is less developed in birds feeding-on-the-wing, than in pecking avians. This was suggested previously. By intraocular horseradish peroxidase applications, we studied the central origin of this retino-petal system in thrush, haw finch, swift and swallow. Our data support the assumption on a corr...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90207-0
更新日期:1994-11-21 00:00:00
abstract::The three tracers horseradish peroxidase (HRP), 4',6-diamidino-2-phenylindol-2HCl (DAPI) and Fast Blue (FB) differ in retrograde labeling of striato-nigral neurons. After a 24 h survival, injection of DAPI into the ventral tegmentum labeled numerous cells throughout the neostriatum, whereas an identical amount of HRP ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(83)90025-3
更新日期:1983-07-29 00:00:00
abstract:AIMS:To test the hypothesis that uridine 5'-triphosphate (UTP) had a protective effect on cerebral ischemia reperfusion (IR) injury in rats. METHODS:Ischemia was induced by intraluminal suture of middle cerebral artery occlusion (MCAO). UTP solution was delivered through an indwelling tail venous catheter via microinf...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.08.076
更新日期:2009-11-06 00:00:00
abstract::The potential of nitric oxide for the induction of reactive oxygen species (ROS) has been evaluated in a cerebral synaptosomal fraction. Nitric oxide (NO) was generated using S-nitroso-N-acetylpenicillamine (SNAP). NO stimulated ROS production, assayed using a fluorescent probe. This stimulation was largely inhibited ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90409-x
更新日期:1994-02-28 00:00:00
abstract::Depression associated with diabetes has been described as a highly debilitating comorbidity. Due to its complex and multifactorial mechanisms, the treatment of depression associated with diabetes represents a clinical challenge. Cannabidiol (CBD), the non-psychotomimetic compound derived from Cannabis sativa, has been...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2018.06.006
更新日期:2018-08-24 00:00:00
abstract::Antidromic action potentials were recorded from rat paraventricular nucleus (PVN) and supraoptic nucleus neurons following electrical stimulation of the pituitary stalk. Increases in stimulation frequency to 10 or 20 Hz caused progressive increases in antidromic latency and, often, failure of the antidromic potential ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(83)90437-8
更新日期:1983-06-30 00:00:00
abstract::Unilateral movements are enabled through a distributed network of motor cortical areas but the relative contribution from the parts of this network is largely unknown. Failure of this network potentially results in mirror activation of the primary motor cortex (M1) ipsilateral to the intended movement. Here we tested ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.06.003
更新日期:2004-09-02 00:00:00
abstract::The enzyme Fatty Acid Amide Hydrolase (FAAH) is a key regulator of the endogenous levels of a family of biologically active lipid mediators, the fatty acid amides. These include anandamide, oleoyl ethanolamide and palmitoyl ethanolamide, and their effects are mediated by a variety of downstream targets including canna...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.04.090
更新日期:2008-07-04 00:00:00
abstract::As overweight and obesity are a growing problem in industrialized societies, they become a main focus of many studies. The aim of this study was to determine whether there is an association between the occurrence of polymorphisms in serotonin-related genes and the prevalence of depressive symptoms in obese patients. T...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2014.12.012
更新日期:2015-01-23 00:00:00
abstract::Pharmacological agents acting at alpha-2 adrenergic receptors are widely used in physiology and neuroscience research. Mounting evidence of their potential utility in clinical and experimental psychopharmacology, necessitates new models and novel model organisms for their screening. Here, we characterize behavioral ef...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2019.03.001
更新日期:2019-05-14 00:00:00
abstract:BACKGROUND AND PURPOSE:The pathophysiology of cerebral aneurysms (CA) is linked to chronic inflammation. Endothelial damage is one of the first changes in CA walls resulted from inflammation. It has been shown that increase in plasma homocysteine (Hcy) impairs vascular endothelium and correlates with the development of...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2011.02.076
更新日期:2011-04-25 00:00:00
abstract::Receptor gated Ca2+ entry has been associated with transient receptor potential (TRP) proteins encoded by several different genes. Here, we compare expression of mRNA for TRP isoforms encoded by genes TRP1-6 in the rat substantia nigra and whole brain. The substantia nigra and the whole brain expressed mRNA predominan...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)01533-6
更新日期:2001-03-09 00:00:00
abstract::Treatment with dopamine and other dopamine D2 receptor agonists has been shown to induce cell death through activation of caspase-3 pathway. However, initial step that leads to the activation of caspase-3 in D2 receptor-mediated apoptotic pathway remains unclear. Recently, it was shown that a spliced variant of Galpha...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.04.078
更新日期:2008-07-04 00:00:00
abstract::The existence of long-term effects of dark-rearing on visuo-motor coordination is still controversial. In this study 2 dark-reared (DR) cats were trained, after 5-6 years of recovery, to perform a reaching movement towards a stationary or a moving target. The accuracy, and the reaction and movement times were evaluate...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90626-k
更新日期:1990-10-16 00:00:00
abstract::It is widely held that transdiaphragmatic pressure is a reliable index of the extent of central activation of the diaphragm but the maximal voluntary transdiaphragmatic pressure is lower during inspiratory than expulsive efforts. To determine whether the diaphragm is fully activated during the two manoeuvres supramaxi...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90481-8
更新日期:1988-06-17 00:00:00
abstract::Transient retinal fibers to the inferior colliculus were found in newborn albino rats. After horseradish peroxidase injection into the vitreous cavity of rats of 1-3 days of age, anterogradely-labeled retinal fibers were traced through the superior colliculus to the inferior colliculus. These retinal fibers to the inf...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(83)90496-2
更新日期:1983-05-27 00:00:00
abstract::Rhythms of plasma melatonin levels were determined in lizards (Tiliqua rugosa) subjected to a 12 h photocycle (12 h light: 12 h dark) at constant 33 degrees C, and at 7 different thermoperiods (33 degrees C thermophase and 15 degrees C cryophase) whose thermophase duration ranged from 1.5 to 21 h. The melatonin secret...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(91)90669-k
更新日期:1991-01-02 00:00:00
abstract::The influence of monokines and CNS-derived colony-stimulating factors (CSF) on the growth of microglia has been studied in mixed glial primary cultures stemming from mouse embryos. We observed that spontaneous growth of microglial cells in the presence of astrocytes is blocked by adding anti-colony-stimulating factor ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90534-r
更新日期:1993-02-19 00:00:00