Localization of dopamine D2 receptor mRNA with non-radioactive in situ hybridization histochemistry.

Abstract:

:A digoxigenin-labeled antisense 42-mer oligonucleotide was used for the localization of the dopamine D2 receptor mRNA in the rat brain. The digoxigenin label was identified with alkaline phosphatase conjugated sheep-anti-digoxigenin. In good analogy with the known terminal fields of the dopaminergic system, various nuclei throughout the brain were labeled. Positive in situ hybridization signals were also found in dopamine cell groups of the substantia nigra and ventral tegmental area and in regions where a dopaminergic innervation is controversial, like the cerebellar cortex and the hippocampus. The non-radioactive in situ hybridization procedure described, shows the localization of the dopamine D2 receptor mRNA with a very high contrast and an optimal histological resolution.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Brouwer N,Van Dijken H,Ruiters MH,Van Willigen JD,Ter Horst GJ

doi

10.1016/0304-3940(92)90378-k

keywords:

subject

Has Abstract

pub_date

1992-08-17 00:00:00

pages

223-7

issue

2

eissn

0304-3940

issn

1872-7972

pii

0304-3940(92)90378-K

journal_volume

142

pub_type

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