Abstract:
:Fascin-1 is a putative bundling factor of actin filaments in the filopodia of neuronal growth cones. Here, we examined the structure of the actin bundle formed by human fascin-1 (actin/fascin bundle), and its mode of interaction with myosin in vitro. The distance between cross-linked filaments in the actin/bundle was 8-9 nm, and the bundle showed the transverse periodicity of 36 nm perpendicular to the bundle axis, which was confirmed by electron microscopy. Decoration of the actin/fascin bundle with heavy meromyosin revealed that the arrowheads of filaments in the bundle pointed in the same direction, indicating that the bundle has polarity. This result suggested that fascin-1 plays an essential role in polarity of actin bundles in filopodia. In the in vitro motility assay, actin/fascin bundles slid as fast as single actin filaments on myosin II and myosin V. When myosin was attached to the surface at high density, the actin/fascin bundle disassembled to single filaments at the pointed end of the bundle during sliding. These results suggest that myosins may drive filopodial actin bundles backward by interacting with actin filaments on the surface, and may induce disassembly of the bundle at the basal region of filopodia.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Ishikawa R,Sakamoto T,Ando T,Higashi-Fujime S,Kohama Kdoi
10.1046/j.1471-4159.2003.02058.xkeywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
676-85issue
3eissn
0022-3042issn
1471-4159pii
2058journal_volume
87pub_type
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journal_title:Journal of neurochemistry
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