当前位置： SCI文献检索 > INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE期刊下所有文献 > Gene therapy with brain-derived neurotrophic factor as a protection: retinal ganglion cells in a rat glaucoma model.

Gene therapy with brain-derived neurotrophic factor as a protection: retinal ganglion cells in a rat glaucoma model.

Abstract：

PURPOSE:To develop a modified adenoassociated viral (AAV) vector capable of efficient transfection of retinal ganglion cells (RGCs) and to test the hypothesis that use of this vector to express brain-derived neurotrophic factor (BDNF) could be protective in experimental glaucoma. METHODS:Ninety-three rats received one unilateral, intravitreal injection of either normal saline (n = 30), AAV-BDNF-woodchuck hepatitis posttranscriptional regulatory element (WPRE; n = 30), or AAV-green fluorescent protein (GFP)-WPRE (n = 33). Two weeks later, experimental glaucoma was induced in the injected eye by laser application to the trabecular meshwork. Survival of RGCs was estimated by counting axons in optic nerve cross sections after 4 weeks of glaucoma. Transgene expression was assessed by immunohistochemistry, Western blot analysis, and direct visualization of GFP. RESULTS:The density of GFP-positive cells in retinal wholemounts was 1,828 +/- 299 cells/mm(2) (72,273 +/- 11,814 cells/retina). Exposure to elevated intraocular pressure was similar in all groups. Four weeks after initial laser treatment, axon loss was 52.3% +/- 27.1% in the saline-treated group (n = 25) and 52.3% +/- 24.2% in the AAV-GFP-WPRE group (n = 30), but only 32.3% +/- 23.0% in the AAV-BDNF-WPRE group (n = 27). Survival in AAV-BDNF-WPRE animals increased markedly and the difference was significant compared with those receiving either AAV-GFP-WPRE (P = 0.002, t-test) or saline (P = 0.006, t-test). CONCLUSIONS:Overexpression of the BDNF gene protects RGC as estimated by axon counts in a rat glaucoma model, further supporting the potential feasibility of neurotrophic therapy as a complement to the lowering of IOP in the treatment of glaucoma.

journal_name

Invest Ophthalmol Vis Sci

journal_title

Investigative ophthalmology & visual science

authors

Martin KR,Quigley HA,Zack DJ,Levkovitch-Verbin H,Kielczewski J,Valenta D,Baumrind L,Pease ME,Klein RL,Hauswirth WW

doi

10.1167/iovs.02-1332

keywords:

subject

Has Abstract

pub_date

2003-10-01 00:00:00

pages

4357-65

issue

10

eissn

0146-0404

issn

1552-5783

journal_volume

44

pub_type

杂志文章

相关文献

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE文献大全
  • The Effect of Light Level and Small Pupils on Presbyopic Reading Performance.

    abstract：Purpose:To examine the impact of small pupils and light levels on reading performance of distance-corrected presbyopes. To determine whether small pupils would enable presbyopes to read at near even at low light levels. Methods:To establish the lower range of text luminances, we quantified the space-averaged luminance...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-20008

    authors： Xu R,Gil D,Dibas M,Hare W,Bradley A

    更新日期：2016-10-01 00:00:00

  • Anti-Inflammatory Effect of Dexamethasone Controlled Released From Anterior Suprachoroidal Polyurethane Implants on Endotoxin-Induced Uveitis in Rats.

    abstract：PURPOSE:Targeted drug delivery to the ocular tissues remains a challenge. Biodegradable intraocular implants allow prolonged controlled release of drugs directly into the eye. In this study, we evaluated an anterior suprachoroidal polyurethane implant containing dexamethasone polyurethane dispersions (DX-PUD) as a drug...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.15-18127

    authors： Barbosa Saliba J,Vieira L,Fernandes-Cunha GM,Rodrigues Da Silva G,Ligório Fialho S,Silva-Cunha A,Bousquet E,Naud MC,Ayres E,Oréfice RL,Tekaya M,Kowalczuk L,Zhao M,Behar-Cohen F

    更新日期：2016-04-01 00:00:00

  • Neural rim characteristics of healthy South Indians: the Chennai Glaucoma Study.

    abstract：PURPOSE:To report neural rim dimensions for South Indians and examine rim shape with relevance to clinical evaluation. METHODS:Healthy phakic participants (n = 623) of the Chennai Glaucoma Study with normal frequency-doubling perimetry underwent complete eye examinations including optic disc digital stereophotography....

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.07-1210

    authors： Arvind H,George R,Raju P,Ve RS,Mani B,Kannan P,Vijaya L

    更新日期：2008-08-01 00:00:00

  • Vascular Adhesion Protein-1 Blockade Suppresses Ocular Inflammation After Retinal Laser Photocoagulation in Mice.

    abstract：Purpose:To investigate the effect of the vascular adhesion protein-1 (VAP-1) inhibitor RTU-1096 on retinal morphologic changes and ocular inflammation after retinal laser photocoagulation in mice. Methods:C57BL/6JJcl mice were fed a diet containing RTU-1096, a specific inhibitor for VAP-1, or a control diet ad libitum...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.17-21555

    authors： Matsuda T,Noda K,Murata M,Kawasaki A,Kanda A,Mashima Y,Ishida S

    更新日期：2017-06-01 00:00:00

  • The Properties of Outer Retinal Band Three Investigated With Adaptive-Optics Optical Coherence Tomography.

    abstract：Purpose:Optical coherence tomography's (OCT) third outer retinal band has been attributed to the zone of interdigitation between RPE cells and cone outer segments. The purpose of this paper is to investigate the structure of this band with adaptive optics (AO)-OCT. Methods:Using AO-OCT, images were obtained from two s...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-21138

    authors： Jonnal RS,Gorczynska I,Migacz JV,Azimipour M,Zawadzki RJ,Werner JS

    更新日期：2017-09-01 00:00:00

  • Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator.

    abstract：PURPOSE:To investigate whether tissue plasminogen activator (tPA) can prevent and/or reverse steroid-induced IOP elevation in an ovine model. METHODS:Three animal groups were subjected to bilateral steroid-induced IOP elevation using thrice daily topical ocular prednisolone administration. In the first group (N = 8), ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.13-12801

    authors： Gerometta R,Kumar S,Shah S,Alvarez L,Candia O,Danias J

    更新日期：2013-12-03 00:00:00

  • The effect of platelet-activating factor (PAF), histamine, and ethanol on vascular permeability of the guinea pig conjunctiva.

    abstract：:Increased vascular permeability, one of the characteristic features of immediate hypersensitivity (Type I), is mediated through a variety of compounds, including histamine and platelet-activating factor (PAF), a phospholipid inflammatory mediator. The effects on vascular permeability of histamine, PAF, and ethanol, th...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Stock EL,Roth SI,Kim ED,Walsh MK,Thamman R

    更新日期：1990-05-01 00:00:00

  • Increased plasma levels of substance P in vernal keratoconjunctivitis.

    abstract：PURPOSE:The increase of nerve growth factor (NGF) plasma levels in vernal keratoconjunctivitis (VKC) patients has been demonstrated previously. Results of numerous studies in vitro and in vivo have shown that NGF modulates the synthesis of substance P (SP), a neuropeptide involved in the pathogenesis of human allergic ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Lambiase A,Bonini S,Micera A,Tirassa P,Magrini L,Bonini S,Aloe L

    更新日期：1997-09-01 00:00:00

  • Comprehensive Molecular Diagnosis of a Large Chinese Leber Congenital Amaurosis Cohort.

    abstract：PURPOSE:Leber congenital amaurosis (LCA) is an inherited retinal disease that causes early-onset severe visual impairment. To evaluate the mutation spectrum in the Chinese population, we performed a mutation screen in 145 Chinese LCA families. METHODS:First, we performed direct Sanger sequencing of 7 LCA disease genes...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.14-15972

    authors： Wang H,Wang X,Zou X,Xu S,Li H,Soens ZT,Wang K,Li Y,Dong F,Chen R,Sui R

    更新日期：2015-06-01 00:00:00

  • Revalidating the Usefulness of a "Sector-Wise Regression" Approach to Predict Glaucomatous Visual Function Progression.

    abstract：PURPOSE:We previously developed a method to measure visual function progression using "Progression sectors," which were derived from a clustering analysis of pointwise rates of glaucomatous visual field (VF) progression; however, only short series of VFs, where pointwise linear regression (PLR) is typically not reliabl...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.15-16694

    authors： Hirasawa K,Murata H,Asaoka R

    更新日期：2015-07-01 00:00:00

  • Rho/ROCK signaling in regulation of corneal epithelial cell cycle progression.

    abstract：PURPOSE:The authors' previous study showed that the expression of a Rho-associated serine/threonine kinase (ROCK) is regulated during cell cycle progression in corneal epithelial cells. The present study was conducted to determine whether and how Rho/ROCK signaling regulates cell cycle progression. METHODS:Rabbit corn...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.07-0488

    authors： Chen J,Guerriero E,Lathrop K,SundarRaj N

    更新日期：2008-01-01 00:00:00

  • Quality of life in patients with leber hereditary optic neuropathy.

    abstract：PURPOSE:Leber hereditary optic neuropathy (LHON) is an inherited mitochondrial optic neuropathy characterized by bilateral, severe loss of central vision. In this study, the first formal assessment was conducted of visual disability in affected and unaffected individuals from molecularly confirmed LHON pedigrees. METH...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.08-3166

    authors： Kirkman MA,Korsten A,Leonhardt M,Dimitriadis K,De Coo IF,Klopstock T,Griffiths PG,Hudson G,Chinnery PF,Yu-Wai-Man P

    更新日期：2009-07-01 00:00:00

  • Major depressive and anxiety disorders in visually impaired older adults.

    abstract：PURPOSE:We assessed the prevalence of subthreshold depression and anxiety, and major depressive, dysthymic, and anxiety disorders (panic disorder, agoraphobia, social phobia, and general anxiety disorder) in visually impaired older adults and compared these estimates with those of normally sighted peers. METHODS:Cross...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,多中心研究

    doi：10.1167/iovs.14-15848

    authors： van der Aa HP,Comijs HC,Penninx BW,van Rens GH,van Nispen RM

    更新日期：2015-01-20 00:00:00

  • Proteinase and growth factor alterations revealed by gene microarray analysis of human diabetic corneas.

    abstract：PURPOSE:To identify proteinases and growth factors abnormally expressed in human corneas of donors with diabetic retinopathy (DR), additional to previously described matrix metalloproteinase (MMP)-10 and -3 and insulin-like growth factor (IGF)-I. METHODS:RNA was isolated from 35 normal, diabetic, and DR autopsy human ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.04-1507

    authors： Saghizadeh M,Kramerov AA,Tajbakhsh J,Aoki AM,Wang C,Chai NN,Ljubimova JY,Sasaki T,Sosne G,Carlson MR,Nelson SF,Ljubimov AV

    更新日期：2005-10-01 00:00:00

  • Reattachment of cultured human retinal pigment epithelium to extracellular matrix and human Bruch's membrane.

    abstract：PURPOSE:To determine the mechanism of reattachment of harvested human retinal pigment epithelium (RPE) to RPE-derived extracellular matrix and Bruch's membrane. METHODS:Confluent first-to third-passage human RPE were harvested from tissue culture and plated onto RPE-derived extracellular matrix or human Bruch's membra...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Ho TC,Del Priore LV

    更新日期：1997-05-01 00:00:00

  • Effects of Tear Film Instability on Sensory Responses to Corneal Cold, Mechanical, and Chemical Stimuli.

    abstract：Purpose:To investigate the effects of tear film instability (TFI) induced by sustained tear exposure (STARE) on sensory responses to corneal cold, mechanical, and chemical stimuli. Methods:Fifteen normal subjects were enrolled. TFI was induced during 10 repeated trials of STARE. Pneumatic cold, mechanical, and chemica...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.19-27298

    authors： Situ P,Begley CG,Simpson TL

    更新日期：2019-07-01 00:00:00

  • A functional polymorphism at the FGF10 gene is associated with extreme myopia.

    abstract：PURPOSE:Fibroblast growth factor-10 (FGF10) can modulate extracellular matrix associated genes and, therefore, it could be a myopia susceptibility gene. This study used an animal model, single nucleotide polymorphisms (SNPs) association, and genetic functional assay to evaluate FGF10 gene for myopia. METHODS:The expre...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.13-11814

    authors： Hsi E,Chen KC,Chang WS,Yu ML,Liang CL,Juo SH

    更新日期：2013-05-07 00:00:00

  • Incidence, Long-Term Visual Outcomes, and Mortality in Retinopathy of Prematurity in Korea: A Nationwide Population-Based Study.

    abstract：Purpose:To evaluate the incidence, visual prognosis, and mortality in retinopathy of prematurity (ROP) in Korea. Methods:We used the National Health Insurance and the Korean Disability Registry database, which covers the entire newborn population in 2006 to 2014 and includes information on all newborns diagnosed with ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.61.10.14

    authors： Na KH,Kim KH,Kang TU,Hann HJ,Ahn HS,Kim HJ

    更新日期：2020-08-03 00:00:00

  • Molecular characterization of the skate peripherin/rds gene: relationship to its orthologues and paralogues.

    abstract：PURPOSE:A great deal of information about functionally significant domains of a protein may be obtained by comparison of primary sequences of gene homologues over a broad phylogenetic base. This study was designed to identify evolutionarily conserved domains of the photoreceptor disc membrane protein peripherin/rds by ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.02-1152

    authors： Li C,Ding XQ,O'Brien J,Al-Ubaidi MR,Naash MI

    更新日期：2003-06-01 00:00:00

  • Cell penetration peptides for enhanced entry of αB-crystallin into lens cells.

    abstract：PURPOSE:The prevalence of cataract increases with age. Conversely, the abundance of native α-crystallin diminishes with age and cataract development. We hypothesize replenishing lens α-crystallin may delay or prevent cataract. Herein we investigated the ability of cell penetration peptides (CPP) to enhance entry of α-c...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.12-10947

    authors： Mueller NH,Ammar DA,Petrash JM

    更新日期：2013-01-02 00:00:00

  • Clinical assessment of mirror artifacts in spectral-domain optical coherence tomography.

    abstract：:PURPOSE. To investigate the characteristics of a spectral-domain optical coherence tomography (SD-OCT) image phenomenon known as the mirror artifact, calculate its prevalence, analyze potential risk factors, measure severity, and correlate it to spherical equivalent and central visual acuity (VA). METHODS. OCT macular...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.09-4057

    authors： Ho J,Castro DP,Castro LC,Chen Y,Liu J,Mattox C,Krishnan C,Fujimoto JG,Schuman JS,Duker JS

    更新日期：2010-07-01 00:00:00

  • Lenticular levels of amino acids and free UV filters differ significantly between normals and cataract patients.

    abstract：PURPOSE:To determine the levels of free UV filters and selected amino acids in cataract lenses compared with normal lenses. METHODS:Indian cataract lenses (n=39) and normal lenses (n=6) were examined by HPLC to quantify levels of UV filter compounds, the UV filter precursor amino acid tryptophan (Trp), as well as tyro...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.04-0178

    authors： Streete IM,Jamie JF,Truscott RJ

    更新日期：2004-11-01 00:00:00

  • Sigma-1 Receptor Regulates Mitochondrial Function in Glucose- and Oxygen-Deprived Retinal Ganglion Cells.

    abstract：Purpose:Understanding the role of mitochondria in retinal ganglion cells (RGCs) is relevant to human disease as studies have shown mitochondrial abnormalities in primary open-angle glaucoma patients. This study seeks to determine the effects of the sigma-1 receptor (σ-1r) and its agonists on mitochondrial function in o...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-19199

    authors： Ellis DZ,Li L,Park Y,He S,Mueller B,Yorio T

    更新日期：2017-05-01 00:00:00

  • Changes in Blood Pressure and Urine Albumin-Creatinine Ratio in a Randomized Clinical Trial Comparing Aflibercept, Bevacizumab, and Ranibizumab for Diabetic Macular Edema.

    abstract：Purpose:To compare blood pressure and urine albumin-creatinine ratio over time for participants receiving aflibercept, bevacizumab, or ranibizumab. Methods:Preplanned secondary analyses from a randomized trial comparing aflibercept, bevacizumab, and ranibizumab for diabetic macular edema (DME). The Diabetic Retinopath...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,随机对照试验

    doi：10.1167/iovs.17-22853

    authors： Glassman AR,Liu D,Jampol LM,Sun JK,Diabetic Retinopathy Clinical Research Network.

    更新日期：2018-03-01 00:00:00

  • Recharacterization of the RGC-5 retinal ganglion cell line.

    abstract：PURPOSE:The transformed RGC-5 retinal ganglion cell line is used widely in glaucoma research. Increased resistance to glutamate was noted in published literature and led to the recharacterization of the RGC-5 cell line. METHODS:Characterization of the RGC-5 cell line was performed by sequencing of a region of the nucl...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.09-3484

    authors： Van Bergen NJ,Wood JP,Chidlow G,Trounce IA,Casson RJ,Ju WK,Weinreb RN,Crowston JG

    更新日期：2009-09-01 00:00:00

  • Polyamines in cultured rabbit corneal cells.

    abstract：PURPOSE:To determine whether polyamines are present in corneal cells, whether corneal cell polyamines can be depleted by blocking the first rate-limiting enzyme in the polyamine synthesis pathway, ornithine decarboxylase (ODC), and whether polyamines are required for proliferation in all three corneal cell types. METH...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.02-0889

    authors： Du H,Viar MJ,Johnson LR,Watsky MA

    更新日期：2003-06-01 00:00:00

  • Continuous measurement of intraocular pressure in rabbits by telemetry.

    abstract：PURPOSE:Intraocular pressure (IOP) is dynamic and can be influenced by the use of tonometers. The authors developed a technique to implant a telemetric pressure transducer to measure IOP continuously in unrestrained rabbits. METHODS:A commercial telemetric pressure transducer was implanted subcutaneously on the dorsal...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： McLaren JW,Brubaker RF,FitzSimon JS

    更新日期：1996-05-01 00:00:00

  • The effect of an angiostatic steroid on neovascularization in a rat model of retinopathy of prematurity.

    abstract：PURPOSE:The inhibition of angiogenesis by angiostatic steroids has been demonstrated in a variety of systems, including rabbit and rat cornea. There is considerable interest in the therapeutic potential of this class of compounds for angiogenic ocular conditions such as diabetic retinopathy, macular degeneration, and r...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Penn JS,Rajaratnam VS,Collier RJ,Clark AF

    更新日期：2001-01-01 00:00:00

  • Regional differences in the fine structure of the ciliary epithelium related to accommodation.

    abstract：:The ciliary bodies of five monkey eyes and one human eye were subdivided into five zones. The ciliary epithelium with its bordering stroma was investigated electron microscopically. The number of cell organelles of the nonpigmented (NPE) and pigmented (PE) epithelium (mitochondria, rough endoplasmic reticulum, Golgi c...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Ober M,Rohen JW

    更新日期：1979-07-01 00:00:00

  • Characterization of retinoblastoma immune complexes.

    abstract：:Immune complexes from retinoblastoma sera were characterized with molecular sieve chromatography, affinity chromatography, and polyacrylamide gel electrophoresis (PAGE). Retinoblastoma patients' sera had two well-defined peaks of immune complex activity after molecular sieve chromatography. These protein fractions had...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Stein PC,Christensen M,Char DH

    更新日期：1980-03-01 00:00:00