Abstract:
:This article reviews the progress of a personal endeavour to develop chromatography as a quantitative procedure for the determination of reaction stoichiometries and equilibrium constants governing protein interactions. As well as affording insight into an aspect of chromatography with which many protein chemists are unfamiliar, it shows the way in which minor adaptations of conventional chromatographic practices have rendered the technique one of the most powerful methods available for the characterization of interactions. That pathway towards quantification is followed from the introduction of frontal gel filtration for the study of protein self-association to the characterization of ligand binding by the biosensor variant of quantitative affinity chromatography.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Winzor DJdoi
10.1042/bst0311010keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
1010-4issue
Pt 5eissn
0300-5127issn
1470-8752journal_volume
31pub_type
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