Redefining cholesterol's role in the mechanism of the cholesterol-dependent cytolysins.

Abstract:

:The cholesterol-dependent cytolysins (CDCs) constitute a large family of pore-forming toxins that function exclusively on cholesterol-containing membranes. A detailed analysis of the various stages in the cytolytic mechanism of three members of the CDC family revealed that significant depletion of cholesterol from the erythrocyte membrane stalls these toxins in the prepore complex. Therefore, the depletion of membrane cholesterol prevents the insertion of the transmembrane beta-barrel and pore formation. These unprecedented findings provide a paradigm for the involvement of cholesterol in the CDC cytolytic mechanism and that of other pore-forming toxins whose activity is enhanced by the presence of membrane cholesterol.

authors

Giddings KS,Johnson AE,Tweten RK

doi

10.1073/pnas.2033520100

keywords:

subject

Has Abstract

pub_date

2003-09-30 00:00:00

pages

11315-20

issue

20

eissn

0027-8424

issn

1091-6490

pii

2033520100

journal_volume

100

pub_type

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