Abstract:
:The cholesterol-dependent cytolysins (CDCs) constitute a large family of pore-forming toxins that function exclusively on cholesterol-containing membranes. A detailed analysis of the various stages in the cytolytic mechanism of three members of the CDC family revealed that significant depletion of cholesterol from the erythrocyte membrane stalls these toxins in the prepore complex. Therefore, the depletion of membrane cholesterol prevents the insertion of the transmembrane beta-barrel and pore formation. These unprecedented findings provide a paradigm for the involvement of cholesterol in the CDC cytolytic mechanism and that of other pore-forming toxins whose activity is enhanced by the presence of membrane cholesterol.
journal_name
Proc Natl Acad Sci U S Aauthors
Giddings KS,Johnson AE,Tweten RKdoi
10.1073/pnas.2033520100keywords:
subject
Has Abstractpub_date
2003-09-30 00:00:00pages
11315-20issue
20eissn
0027-8424issn
1091-6490pii
2033520100journal_volume
100pub_type
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