Abstract:
:The GTPase activating proteins (GAP) stimulate the intrinsic GTPase activity of ras-p21 thus converting the protein into its inactive form. We have now analyzed carcinogen-induced mouse liver tumors for the possible occurrence of mutational changes in one of the two known GAP genes, namely p120-GAP. RNA from a total of 21 tumors was reverse transcribed by use of GAP-specific primers, amplified by PCR and sequenced. All known functional domains of p120-GAP were included into the analysis. None of the liver tumors analyzed was found to be mutated within these regions of the gene. Moreover, Southern blot analysis of the gene did not reveal any structural changes. However, at five positions we discovered deviations from the published mouse fibroblast sequence, including two mouse strain-specific sequence polymorphisms.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Müller O,Kress S,Schwarz Mdoi
10.1093/carcin/13.10.1903keywords:
subject
Has Abstractpub_date
1992-10-01 00:00:00pages
1903-5issue
10eissn
0143-3334issn
1460-2180journal_volume
13pub_type
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