Are vertebrate exons scanned during splice-site selection?

Abstract:

:Pairwise recognition of splice sites as a result of a scanning mechanism is an attractive model to explain the coordination of vertebrate splicing. Such a mechanism would predict a polarity-of-site recognition in the scanned unit, but no evidence for a polarity gradient across introns has been found. We have suggested that the exon rather than the intron is the unit of recognition in vertebrates and that polyadenylation and splicing factors interact during recognition of 3'-terminal exons. Interaction is reflected in maximal rates of in vitro polyadenylation. If scanning across the exon is operating during this interaction, then insertion of a 5' splice site should depress polyadenylation. Here we report recognition in vitro and in vivo of a 5' splice site situated within a 3'-terminal exon, and a concomitant depression of polyadenylation and ultraviolet crosslinking of a polyadenylation factor. Decreased crosslinking was only found when the 3' and 5' splice sites were within 300 nucleotides of each other. These results are consistent with an exon scanning mechanism for splice-site selection.

journal_name

Nature

journal_title

Nature

authors

Niwa M,MacDonald CC,Berget SM

doi

10.1038/360277a0

keywords:

subject

Has Abstract

pub_date

1992-11-19 00:00:00

pages

277-80

issue

6401

eissn

0028-0836

issn

1476-4687

journal_volume

360

pub_type

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