Abstract：
:We have developed a simian virus 40 (SV40) T-antigen immortalized human cell line, 1MR90-D305.2H4 (IDH4), in which the expression of T-antigen is controlled by the mouse mammary tumor virus (MMTV) promoter and thus regulated by steroids such as dexamethasone. Studies on the regulation of proliferation by T-antigen led to the formulation of a two-stage model for human cell immortalization, in which a mortality stage 1 mechanism (M1) was the target of T-antigen action, and an independent mortality stage 2 mechanism (M2) produced crisis and prevented T-antigen from directly immortalizing cells. Rarely, a cell expressing T-antigen escaped crisis (e.g., M2) and was capable of indefinite proliferation. This model predicted that the deinduction of T-antigen in IDH4 cells would lead to the reexpression of the M1 mechanism, and thus a reexpression of the senescent phenotype. Our study confirms the prediction that, in the absence of steroids, IDH4 cells express a variety of morphological and biochemical markers characteristic of normal senescent human fibroblasts.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Shay JW,West MD,Wright WEdoi
10.1016/0531-5565(92)90003-ikeywords:
subject
Has Abstractpub_date
1992-09-01 00:00:00pages
477-92issue
5-6eissn
0531-5565issn
1873-6815journal_volume
27pub_type
杂志文章abstract：:Recent deep sequencing studies on T-cell receptor (TCR) repertoire have provided robust data to characterize diversity of T-cell immune responsiveness to a wide variety of peptide antigens, including viral and tumor antigens. The human TCR repertoire declines with age, but this decline has not been fully investigated ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2017.05.015
更新日期：2017-10-01 00:00:00
abstract：:In muscles, sarcopenia, the loss of muscle mass, is the major cause of aging-related functional decline and frailty. Several factors are correlated with sarcopenia during aging, including contraction-related cellular injury, oxidative stress, endocrine changes and reduced regenerative potential. However the involvemen...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2005.12.004
更新日期：2006-03-01 00:00:00
abstract：:Light and electron microscope comparisons were made of parotid and submandibular glands from male Swiss-Webster white mice 3, 13, and 18 months old. The glands from the 13- and 18-month-old mice were less organized and the parenchyma was not as dense. Fibrous connective tissue, intracellular lipofuscin granules, and r...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(88)90007-1
更新日期：1988-01-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common cause of dementia accounting for 60-70% of all demented cases and one of the leading sources of morbidity and mortality in the aging population. Most of the recent literature regards the relationship between plasma oxidative stress and AD, showing that markers of lipid perox...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2019.110754
更新日期：2019-12-01 00:00:00
abstract：:The mechanisms responsible for age-associated hearing loss are still incompletely characterized. In this study, we used a murine model of age-dependent hearing loss and evaluated whether this condition is associated with vascular modifications of the structures of the inner ear. We used old C57BL/6J mice that are affe...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2004.06.003
更新日期：2004-08-01 00:00:00
abstract：:Cinnamon is a spice commonly used worldwide to flavor desserts, fruits, cereals, breads, and meats. Numerous health benefits have been attributed to its consumption, including the recent suggestion that it may decrease blood glucose levels in people with diabetes. Insulin signaling is an integral pathway regulating th...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2014.09.019
更新日期：2014-12-01 00:00:00
abstract：:Collagens and elastin are 'building blocks' of tissues and extracellular matrix. Mutations in these proteins cause severe congenital syndromes. Adverse genetic variations may accelerate the aging process in adults contributing to premature morbidity, disability and/or mortality. Favorable variants may contribute to lo...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2019.110781
更新日期：2020-01-01 00:00:00
abstract：:Age- and sex-associated differences in lipid peroxidation (LPO), and total sialic acid content (TSA) in response to abuse of drugs have been reported both in humans and experimental animals. However, no data on the influence of gender and age on these parameters have been reported for methionine-enkephalin (MENK). In ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(02)00118-3
更新日期：2002-12-01 00:00:00
abstract：:The study examined the influence of sex on the alterations occurring with ageing in rat lymph node (LN) T cell compartment. In female and male rats the decrease in LN T cell counts was followed by a shift in CD4+/CD8+ T cell ratio towards CD8+ T cells, which was more prominent in males than in females. With ageing, in...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2020.111140
更新日期：2020-12-01 00:00:00
abstract：:During the course of life, cyclic females face a state of midlife transition that occurs in a fully functioning neurological system, and results in reproductive senescence. The authors' hypothesis was that changes in the activity noradrenergic neurons may be one of the factors involved in this phenomenon. The aim of t...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2016.04.015
更新日期：2016-08-01 00:00:00
abstract：:Accumulating evidence from diverse experiments, including heterochronic parabiosis-the surgical joining of two animals of different ages-has highlighted the importance of systemic factors in the progressive functional decline of various organs and tissues during aging. The major metabolic pathway of tryptophan, an ess...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/j.exger.2019.110793
更新日期：2020-02-01 00:00:00
abstract：:Reductive stress, which is due to a paradoxical excess of antioxidants such as reduced glutathione (GSH) and GSH-related enzymes associated with decreased oxidant levels, has emerged as a pathogenetic mechanism of myocardial damage in pathological conditions such as protein aggregation cardiomyopathy. Notably, in the ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2020.110871
更新日期：2020-02-06 00:00:00
abstract：:To determine whether age-related differences in body weight loss in hyperthyroidism could be related to caloric intake, the body weight and food consumption of Fischer 344 male rats were monitored every other day for four weeks. Six-month-old (young) rats were compared to 16-month-old rats (intermediate age) and 25-mo...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(90)90006-n
更新日期：1990-01-01 00:00:00
abstract：:Alzheimer's disease (AD) is characterized by amyloid-β plaques, tau pathology and vascular impairment including pericyte damage. Pericytes are perivascular cells of the blood-brain barrier and can differentiate into different cell types in vitro including microglia. The aim of the present study is to explore if primar...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2018.08.003
更新日期：2018-10-02 00:00:00
abstract：:The maximal lifespan (MLS) of mammals is inversely correlated with the peroxidation index, a measure of the proportion and level of unsaturation of polyunsaturated fatty acids (PUFA) in membranes. This relationship is likely related to the fact that PUFA are highly susceptible to damage by peroxidation. Previous compa...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2015.08.011
更新日期：2015-10-01 00:00:00
abstract：:Measuring physiological dysregulation during aging could be a key tool both to understand underlying aging mechanisms and to predict clinical outcomes in patients. However, most existing indices are either circular or hard to interpret biologically. Recently, we showed that statistical distance of 14 common blood biom...
journal_title：Experimental gerontology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.exger.2014.04.016
更新日期：2014-09-01 00:00:00
abstract：BACKGROUNDS:Elucidating the causal effects of common intermediate risk factors on the onset of age-related diseases is indispensable for developing prevention and intervention procedures. METHODS:We conducted two-stage time-to-event Mendelian randomization meta-analyses combining five large-scale longitudinal cohorts ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2017.09.019
更新日期：2018-07-01 00:00:00
abstract：:Gene expression is much altered in aging. We observed age-dependent decline of core clock genes' expression in the whole body of the fruit fly. We hypothesized that inducible overexpression of clock genes (cry, per, tim, cyc and Clk) in the nervous system can improve healthspan of D. melanogaster. We studied the lifes...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2018.11.005
更新日期：2019-03-01 00:00:00
abstract：:Young and old C57BL/6 male mice were given a diet containing a high dose of vitamin E (VE treatment) and its effect on the immune system was examined before and after the exposure to restraint stress. The VE treatment per se gave rise to a slight increase of splenic T cells in percentage and a significant enhancement ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(99)00055-8
更新日期：1999-11-01 00:00:00
abstract：BACKGROUND/OBJECTIVES:Geriatric patients are highly susceptible to infections. While reduced lymphocyte count has been associated with age, other studies found no change in WBC counts with age. Increased circulating white blood cell (WBC) count has been associated with cardiovascular (CV) diseases and frailty but there...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2014.11.016
更新日期：2015-01-01 00:00:00
abstract：:It is well established that during early life the thymus gland and the neuroendocrine system influence each other's maturation. Furthermore, there is a growing body of evidence indicating that the immune and neuroendocrine systems also function as a bidirectional network during adult life. In order to assess possible ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(90)90046-5
更新日期：1990-01-01 00:00:00
abstract：:Cardiovascular function during sleep was studied in 14 (6 males, 8 females) healthy, active, older adults (57-70) years). Noninvasive measures of stroke volume (SV), heart rate (HR), cardiac output (CO), pre-ejection period (PEP), and left ventricular ejection time (LVET) were taken using impedance cardiography and co...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(87)90031-3
更新日期：1987-01-01 00:00:00
abstract：:Cellular aging plays a role in longevity and senescence, and has been implicated in medical and psychiatric conditions, including heart disease, cancer, major depression and posttraumatic stress disorder. Telomere shortening and mitochondrial dysfunction are thought to be central to the cellular aging process. The pre...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2015.04.002
更新日期：2015-06-01 00:00:00
abstract：AIM:The present study investigated whether hypertension, blood pressure, and antihypertensive therapy were associated with frailty status in community-dwelling older adults. In addition, we tested whether such associations were consistent across different frailty instruments. MATERIAL AND METHODS:Two-hundred older adu...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2021.111245
更新日期：2021-01-19 00:00:00
abstract：:Women who seek medical care around the time of menopause frequently report changes in one or more aspects of psychological functioning. The etiology of these symptoms is likely multifactorial and, undoubtedly, individual and sociocultural factors are important determinants. The known psychotropic properties of the sex...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/0531-5565(94)90021-3
更新日期：1994-05-01 00:00:00
abstract：:The intrinsic contractile properties of papillary muscles isolated from female Wistar rats during post-natal development were examined. In addition, the myocardial responsiveness to certain inotropic interventions was studied. In muscles from 60-day-old animals, the velocity of shortening was markedly greater than mus...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(82)90025-0
更新日期：1982-01-01 00:00:00
abstract：OBJECTIVE:Senescent patients exhibit an elevated perioperative risk for cardiac dysfunction, hemodynamic depression and subsequent cardiac death compared to young patients. Despite the fact that a growing proportion of cardiac surgery patients are octogenarians, cardioplegic regimes remain comparable across patients of...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2015.04.012
更新日期：2015-07-01 00:00:00
abstract：:The balance between the activities of matrix metalloproteinases (MMPs) and the tissue inhibitors of metalloproteinases (TIMPs) is an important control point in tissue remodeling. Previous studies have demonstrated elevated expression of the MMPs collagenase and stromelysin-1 by aged human diploid fibroblasts compared ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(95)02010-1
更新日期：1996-01-01 00:00:00
abstract：:It has been often demonstrated that during senescence some neurons undergo atrophic changes while others add new processes and terminals. Because microtubules form a substantial component of the dendritic and axonal cytoskeleton, we have studied the amount of tubulin and acetylated alpha-tubulin in three young (6 mont...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(92)90034-w
更新日期：1992-01-01 00:00:00
abstract：OBJECTIVE:This study aimed to evaluate the effect of vitamin D3 megadose supplementation and influence of BsmI polymorphism in the VDR gene on the inflammatory profile and oxidative stress in elderly women with vitamin D deficiency. METHODS:A double blind, randomized, placebo-controlled trial was conducted with 40 eld...
journal_title：Experimental gerontology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.exger.2015.03.011
更新日期：2015-06-01 00:00:00
