Expression of myosin heavy and light chains and phosphorylation of the phosphorylatable myosin light chain in the heart ventricle of the European hamster during hibernation and in summer.

abstract：:The width (D) and the 1,0 lattice spacing (d1,0) at various ionic compositions of mechanically skinned single fibres (from semitendinosus muscle of Rana catesbeiana) were measured at various concentrations of polyvinyl pyrrolidone (PVP K-30, Mn = 40 000) from 0 to 6% at 20 degrees C. In a standard relaxing solution (4...

journal_title：Journal of muscle research and cell motility

authors： Umazume Y,Onodera S,Higuchi H

更新日期：1986-06-01 00:00:00

abstract：:Myosin binding protein C (MYBPC) is a crucial component of the sarcomere and an important regulator of muscle function. While mutations in different myosin binding protein C (MYBPC) genes are well known causes of various human diseases, such as hypertrophic (HCM) and dilated (DCM) forms of cardiomyopathy as well as sk...

journal_title：Journal of muscle research and cell motility

authors： Knöll R

更新日期：2012-05-01 00:00:00

abstract：:Skeletal muscle repair requires the migration of myoblasts (activated satellite cells) both to the injury site and then within the wound to facilitate cellular alignment in preparation for differentiation, fusion and eventual healing. Along this journey, the cells encounter a range of soluble and extracellular matrix ...

journal_title：Journal of muscle research and cell motility

authors： Goetsch KP,Myburgh KH,Niesler CU

更新日期：2013-12-01 00:00:00

abstract：:The β-adrenergic receptor stimulation improves endurance in fast twitch muscles and these effects are sensitive to extracellular Ca2+ influx. Present study is aimed to determine the effects of adrenaline, with different concentrations of extracellular Ca2+[Formula: see text], on the contractility and endurance of slow...

journal_title：Journal of muscle research and cell motility

authors： Rizvi MH,Abdul Azeem M,Savanur A

更新日期：2019-12-01 00:00:00

abstract：:temporaria (0.9-2.5degrees C) were stimulated to produce a 1 s isometric tetanus at regular intervals until constant mechanical responses were attained. Various degrees of force depression ("fatigue") were produced by decreasing the contraction interval from 30 or 15 min (control to 120, 60, 30 and 15s, respectively. ...

journal_title：Journal of muscle research and cell motility

authors： Edman KA,Mattiazzi AR

更新日期：1981-09-01 00:00:00

abstract：:In this study the spontaneous activities of prescapular lymph nodes, which were isolated from calves, goats and sheep and mesenteric lymph nodes of guinea-pigs were recorded and analysed in the frequency domain. Stretch-evoked contractions of the mesenteric lymph nodes were also recorded and their frequency characteri...

journal_title：Journal of muscle research and cell motility

authors： Tümer A,Oztürk-Demir N,Basar-Eroğlu C,Noyan A

更新日期：1983-02-01 00:00:00

abstract：:Heat rate and tension were measured during the steady state of isovelocity shortening in frog sartorius muscles at two speeds (7.5 and 2.0 mm s-1) through two ranges of the length-tension curve: 1.11 to 1.01 L0 and 1.01 to 0.91 L0. Both heat rate and mechanical power were higher at short length than at long length. Th...

journal_title：Journal of muscle research and cell motility

authors： Gilbert SH

更新日期：1986-04-01 00:00:00

abstract：:Two single mutations, R694N and E45Q, were introduced in the beta isoform of human cardiac myosin to remove permanent salt bridges E45:R694 and E98:R694 in the SH1-SH2 helix of the myosin head. Beta isoform-specific bridges E45:R694 and E98:R694 were discovered in the molecular dynamics simulations of the alpha and be...

journal_title：Journal of muscle research and cell motility

authors： Gargey A,Iragavarapu SB,Grdzelishvili AV,Nesmelov YE

更新日期：2020-09-14 00:00:00

abstract：:Calsequestrin type-1 (CASQ1), the main sarcoplasmic reticulum (SR) Ca(2+) binding protein, plays a dual role in skeletal fibers: a) it provides a large pool of rapidly-releasable Ca(2+) during excitation-contraction (EC) coupling; and b) it modulates the activity of ryanodine receptors (RYRs), the SR Ca(2+) release ch...

journal_title：Journal of muscle research and cell motility

authors： Protasi F,Paolini C,Canato M,Reggiani C,Quarta M

更新日期：2011-12-01 00:00:00

abstract：:Several mutations in the beta-myosin heavy chain gene cause hypertrophic cardiomyopathy. This study investigates (1) the in vitro velocities of translocation of fluorescently-labelled actin by beta-myosin purified from soleus muscle of 30 hypertrophic cardiomyopathy patients with seven distinct beta-myosin heavy chain...

journal_title：Journal of muscle research and cell motility

authors： Cuda G,Fananapazir L,Epstein ND,Sellers JR

更新日期：1997-06-01 00:00:00

abstract：:High speed isovelocity shortening using a servo-controlled lever was performed on isolated whole frog sartorius muscles at long lengths to ensure substantial passive tension. The tension records of unstimulated control experiments were subtracted from the tension records of fully-tetanized experiments on the same musc...

journal_title：Journal of muscle research and cell motility

authors： Seo JS,Krause PC,McMahon TA

更新日期：1994-02-01 00:00:00

abstract：:Skeletal muscle has a remarkable ability to respond to different physical stresses. Loading muscle through exercise, either anaerobic or aerobic, can lead to increases in muscle size and function while, conversely, the absence of muscle loading stimulates rapid decreases in size and function. A principal mediator of t...

journal_title：Journal of muscle research and cell motility

authors： Graham ZA,Gallagher PM,Cardozo CP

更新日期：2015-10-01 00:00:00

abstract：:Clenbuterol is a beta2-adrenoceptor agonist primarily used for treating bronchospasm and alleviating the symptoms of chronic obstructive pulmonary disease (COPD) in the horse. In other species (rats, mice, sheep, and cattle), chronic high doses of clenbuterol (typically in the milligram per kilogram body weight range)...

journal_title：Journal of muscle research and cell motility

authors： Plant DR,Kearns CF,McKeever KH,Lynch GS

更新日期：2003-01-01 00:00:00

abstract：:Full muscle relaxation happens when [Ca2+] falls below the threshold for force activation. Several experimental models, from whole muscle organs and intact muscle down to skinned fibers, have been used to explore the cascade of kinetic events leading to mechanical relaxation. The use of single myofibrils together with...

journal_title：Journal of muscle research and cell motility

authors： Vitale G,Ferrantini C,Piroddi N,Scellini B,Pioner JM,Colombini B,Tesi C,Poggesi C

更新日期：2019-11-19 00:00:00

abstract：:In frog semitendinosus muscle the descending limb of the length-tension curve is shifted rightward relative to that of tibialis anterior. Both the plateau right corner and the zero-force intercept are equally shifted. To investigate the reason for this shift, we compared filament lengths in the two muscles. Single fib...

journal_title：Journal of muscle research and cell motility

authors： Trombitás K,Frey L,Pollack GH

更新日期：1993-04-01 00:00:00

abstract：:The myosin light chains of slow muscles from different species have been examined two-dimensional gel electrophoresis. While the myosin light chain 2S of mammalian soleus muscles (rabbit, rat and guinea-pig) could not be distinguished from that of avian anterior latissimus dorsi (chicken and turkey), the 1S light chai...

journal_title：Journal of muscle research and cell motility

authors： Carraro U,dalla Libera L,Catani C

更新日期：1981-09-01 00:00:00

abstract：:White muscle fibres from dogfish were used to compare the energetic costs of shortening by fully active muscle and by relaxing muscle. The muscle preparation was tetanized for 0.6 s and shortened either during stimulation or during relaxation. The distance shortened was 1 mm (about 15% L0, the muscle length optimum fo...

journal_title：Journal of muscle research and cell motility

authors： Lou F,Curtin NA,Woledge RC

更新日期：1998-10-01 00:00:00

abstract：:The developmental shift in contractile protein isoform expression in the rodent heart likely affects actin-myosin cross-bridge interactions. We compared the Ca2+ sensitivity for force generation and cross-bridge cycling kinetics in neonatal (postnatal days 0-3) and adult (day 84) rats. The force-pCa relationship was d...

journal_title：Journal of muscle research and cell motility

authors： Prakash YS,Cody MJ,Housmans PR,Hannon JD,Sieck GC

更新日期：1999-10-01 00:00:00

abstract：:The ATP-ADP exchange reaction and its related partial reactions of fragmented sarcoplasmic reticulum from bullfrog skeletal muscle (frog FSR) were investigated and compared with those of rabbit FSR in order to understand the characteristics of calcium-activated ATPase (Ca2+-ATPase) of frog FSR. MgATP and magnesium-fre...

journal_title：Journal of muscle research and cell motility

authors： Ogawa Y,Kurebayashi N

更新日期：1982-03-01 00:00:00

abstract：:Much has been written about the effects of aging on reproductive function, especially female fertility. Much less is known about how aging may affect the contractility of the smooth muscle within the uterus, the myometrium. The myometrium is active through a woman's entire life, not just during pregnancy. Here we will...

journal_title：Journal of muscle research and cell motility

authors： Arrowsmith S,Robinson H,Noble K,Wray S

更新日期：2012-08-01 00:00:00

abstract：:The efficacy of the 'EGTA-treatment' for producing a model of selectively 'skinned' cardiac muscle has been questioned. This paper deals with ultrastructural evidence designed to test whether small ions can gain access to the myofibrillar space of the heart after 'EGTA-treatment'. Lanthanum has been employed because o...

journal_title：Journal of muscle research and cell motility

authors： Miller DJ,Elder HY,Smith GL

更新日期：1985-10-01 00:00:00

abstract：:The sarcomere of striated muscle is a very efficient machine transforming chemical energy into movement. However, a wrong distribution of the generated forces may lead to self-destruction of the engine itself. A well-known example for this is eccentric contraction (elongation of the sarcomere in the activated state), ...

journal_title：Journal of muscle research and cell motility

authors： Agarkova I,Ehler E,Lange S,Schoenauer R,Perriard JC

更新日期：2003-01-01 00:00:00

abstract：:A method for quick-freezing muscles while observing their mechanical properties until the moment of freezing is described. This method was used to freeze the anterior byssus retractor muscle (ABRM) of Mytilus edulis. Intact muscle in the presence of sucrose as a cryoprotectant was freeze-substituted in acetone, fixed ...

journal_title：Journal of muscle research and cell motility

authors： Bennett PM,Elliott A

更新日期：1989-08-01 00:00:00

abstract：:Isolated fibres from various muscles were skinned mechanically in oil. From a Ca2+-loaded micropipette, local applications of Ca2+ were made. These produced a limited contraction which relaxed spontaneously. The time-course of sarcomere shortening and re-lengthening was recorded by microcinephotography. Application of...

journal_title：Journal of muscle research and cell motility

authors： Gillis JM

更新日期：1997-08-01 00:00:00

abstract：:Results were obtained from contracting frog muscles by collecting high quality time-resolved, two-dimensional, X-ray diffraction patterns at the British Synchrotron Radiation Source (SERC, Daresbury, Laboratory). The structural transitions associated with isometric tension generation were recorded under conditions in ...

journal_title：Journal of muscle research and cell motility

authors： Bordas J,Diakun GP,Diaz FG,Harries JE,Lewis RA,Lowy J,Mant GR,Martin-Fernandez ML,Towns-Andrews E

更新日期：1993-06-01 00:00:00

abstract：:Ryanodine receptors (RyRs) are huge homotetrameric Ca2+ release channels localized to the sarcoplasmic reticulum. RyRs are responsible for the release of Ca2+ from the SR during excitation-contraction coupling in striated muscle cells. Recent revolutionary advancements in cryo-electron microscopy have provided a numbe...

journal_title：Journal of muscle research and cell motility

authors： Ogawa H,Kurebayashi N,Yamazawa T,Murayama T

更新日期：2020-02-10 00:00:00

abstract：:Gaps in our understanding of muscle mechanics demonstrate that the current model is incomplete. Increasingly, it appears that a role for titin in active muscle contraction might help to fill these gaps. While such a role for titin is increasingly accepted, the underlying molecular mechanisms remain unclear. The goals ...

journal_title：Journal of muscle research and cell motility

authors： Nishikawa K,Dutta S,DuVall M,Nelson B,Gage MJ,Monroy JA

更新日期：2020-03-01 00:00:00

abstract：:We examined the temperature dependence of muscle and non-muscle myosin (heavy meromyosin, HMM) with in vitro motility and actin-activated ATPase assays. Our results indicate that myosin V (MV) has a temperature dependence that is similar in both ATPase and motility assays. We demonstrate that skeletal muscle myosin (S...

journal_title：Journal of muscle research and cell motility

authors： Yengo CM,Takagi Y,Sellers JR

更新日期：2012-12-01 00:00:00

abstract：:Secophalloidin (SPH) is known to cause in cardiac myofibrils force without Ca(2+) (half-maximal effect approximately 2 mM) followed by irreversible loss of Ca(2+)-activated force. At maximal Ca(2+) activation, SPH increases force (half-maximal effect < 0.1 mM). We found that SPH at low concentration (0.5 mM) did not c...

journal_title：Journal of muscle research and cell motility

authors： Bukatina AE,Sieck GC,Campbell KB,Belohlavek M

更新日期：2009-01-01 00:00:00

abstract：:Trabeculae from the right ventricle of rat were chemically 'skinned' with saponin (50 micrograms ml-1 for 30 min). Caffeine (10 mM) induced a transient contracture as a consequence of Ca(2+)-release from the sarcoplasmic reticulum and subsequent activation of the myofilaments. The amplitude of the caffeine contracture...

journal_title：Journal of muscle research and cell motility

authors： Steele DS,Miller DJ

更新日期：1992-04-01 00:00:00