Identification and characterization of chicken interleukin-16 cDNA.

Abstract:

:Interleukin-16 is an inflammatory cytokine synthesized as a precursor protein (pro-IL-16). Based on sequence data from an EST cDNA library prepared from intestinal intraepithelial lymphocytes of Eimeria-infected chickens, we identified a cDNA that contained a full-length open reading frame of pro-IL-16. The encoded protein, predicted to consist of 607 amino acids, showed 86% sequence identity to duck pro-IL-16 and 49-52% identity to various mammalian homologues. By Northern blot analysis, IL-16 transcripts were identified in chicken lymphoid tissues but none of the non-lymphoid tissues examined. A recombinant protein containing the 149 C-terminal amino acids of pro-IL-16, expressed in COS-7 cells, showed chemoattractant activity for splenic lymphocytes.

journal_name

Dev Comp Immunol

authors

Min W,Lillehoj HS

doi

10.1016/s0145-305x(03)00133-2

keywords:

subject

Has Abstract

pub_date

2004-02-01 00:00:00

pages

153-62

issue

2

eissn

0145-305X

issn

1879-0089

pii

S0145305X03001332

journal_volume

28

pub_type

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