Abstract:
:The details of the global and local structure and function of titin, a giant filamentous intrasarcomeric protein are largely undiscovered. Here we discuss a combination of bulk-solution and novel single-molecule techniques that may lend unique insights into titin's molecular dynamic, structural and mechanical characteristics.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Kellermayer MS,Grama Ldoi
10.1023/a:1023414624092keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
499-511issue
5-6eissn
0142-4319issn
1573-2657journal_volume
23pub_type
杂志文章,评审abstract::This study investigated the effects of the protein kinase A (PKA) inhibitor, H-89, in mechanically-skinned muscle fibres and intact muscle fibres, in order to determine whether PKA phosphorylation is essential for normal excitation-contraction (E-C) coupling. In skinned EDL fibres of the rat, force responses to depola...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1012289526618
更新日期:2001-01-01 00:00:00
abstract::We have investigated high-affinity ryanodine-binding sites in membrane preparations from representative fast-twitch and slow-twitch muscles of the rabbit and rat, as well as from human mixed muscle. Our results, obtained in high-ionic strength binding buffer, demonstrate extensive similarities in binding affinity for ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00130421
更新日期:1994-04-01 00:00:00
abstract::Creatine kinase (CK) isoenzyme compositions of different types of single muscle fibres dissected from soleus (SOL) and extensor digitorum longus (EDL) muscles from rats were examined. CK isoenzymes were separated into cytoplasmic (CK-MM, CK-MB, CK-BB) (muscle, brain and hybrid types, respectively) and mitochondrial (m...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01781172
更新日期:1991-02-01 00:00:00
abstract::The pattern of spontaneous skeletal muscle degeneration and clinical recovery hindlimb muscles of the mdx mutant mouse was examined for functional and metabolic confirmation of apparent structural regeneration. The contractile properties, histochemical staining and myosin light chain and parvalbumin contents of extens...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01738755
更新日期:1988-12-01 00:00:00
abstract::In mammalian ventricles, two myosin heavy chain (MHC) isoforms have been identified. Small animals express α-MHC, whereas large animals express β-MHC, which contribute to a large difference in the heart rate. Sprague-Dawley rats possessing ~99% α-MHC were treated with propylthiouracil to result in 100% β-MHC. Papillar...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-016-9456-2
更新日期:2016-12-01 00:00:00
abstract::The β-adrenergic receptor stimulation improves endurance in fast twitch muscles and these effects are sensitive to extracellular Ca2+ influx. Present study is aimed to determine the effects of adrenaline, with different concentrations of extracellular Ca2+[Formula: see text], on the contractility and endurance of slow...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-019-09551-9
更新日期:2019-12-01 00:00:00
abstract::In frog semitendinosus muscle the descending limb of the length-tension curve is shifted rightward relative to that of tibialis anterior. Both the plateau right corner and the zero-force intercept are equally shifted. To investigate the reason for this shift, we compared filament lengths in the two muscles. Single fib...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00115451
更新日期:1993-04-01 00:00:00
abstract::Skeletal muscle hypertrophy is a result of increased load, such as functional and stretch-overload. Activation of satellite cells and proliferation, differentiation and fusion are required for hypertrophy of overloaded skeletal muscles. On the contrary, a dramatic loss of skeletal muscle mass determines atrophy settin...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-010-9204-y
更新日期:2009-12-01 00:00:00
abstract::To date, there has been no report of rat TnC purification, despite the rat being an animal commonly used in physiological studies of mammalian muscle. In this study we isolated the fast and slow Troponin C isoforms from rat extensor digitorum longus (23 microg TnC/g wet weight) and soleus (17.6 microg TnC/g wet weight...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/b:jure.0000009896.78385.16
更新日期:2003-01-01 00:00:00
abstract::The loss of muscle force and power during fatigue from intense contractile activity is associated with, and likely caused by, elevated levels of phosphate ([Formula: see text]) and hydrogen ions (decreased pH). To understand how these deficits in muscle performance occur at the molecular level, we used direct measurem...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-018-9499-7
更新日期:2018-08-01 00:00:00
abstract::The sarcomere length (SL) dependence of the calcium sensitivity of force was measured in skinned single twitch and slow-tonic muscle fibres from frog and toad. Twitch and slow-tonic fibres were characterized by location, appearance, physiological response to calcium and by protein band patterns from sodium-dodecyl-sul...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00132181
更新日期:1993-02-01 00:00:00
abstract::The ability for muscle to repeatedly generate force is limited by fatigue. The cellular mechanisms behind muscle fatigue are complex and potentially include breakdown at many points along the excitation-contraction pathway. In this paper we construct a mathematical model of the skeletal muscle excitation-contraction p...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-007-9125-6
更新日期:2007-01-01 00:00:00
abstract::Numerous biological processes, including muscular contraction, depend upon the mechanical properties of actin filaments. One such property is resistance to bending (flexural rigidity, EI). To estimate EI, we attached the ends of fluorescently labelled actin filaments to two microsphere 'handles' captured in independen...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1018672631256
更新日期:1997-02-01 00:00:00
abstract::The myosin II motor from Dictyostelium discoideum has been engineered to contain single tryptophan residues at strategic locations to probe movements of switch 1 and switch 2. The tryptophan residue at W501 probes movement of the relay helix and indirectly reports on switch 2 movement. This probe suggests that there i...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-005-9004-y
更新日期:2005-01-01 00:00:00
abstract::The distribution of Ca2+ and Mg2+ among the 'regulatory' cation binding sites of troponin (T-sites) and the strong, Ca2+-Mg2+ binding sites of troponin and parvalbumins (P-sites) in the sarcoplasm of a muscle was calculated. At rest, 60% of the T-sites were metal free, while 92% of the P-sites were loaded with Mg2+. I...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00712090
更新日期:1982-12-01 00:00:00
abstract::Invertebrate mini-titins are members of a class of myosin-binding proteins belonging to the immunoglobulin superfamily that may have structural and/or regulatory properties. We have isolated mini-titins from three molluscan sources: the striated and smooth adductor muscles of the scallop, and the smooth catch muscles ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00141557
更新日期:1993-12-01 00:00:00
abstract::It is generally thought that the rapid relaxation of fast muscles is facilitated by the Ca(2+) binding protein parvalbumin (Parv). Indeed superfast swimbladder (SWB) muscle of toadfish contains the largest concentration of this protein ever observed (up to 1.5 mM). At 15 degrees C toadfish perform a 100 Hz call, 400 m...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-009-9175-z
更新日期:2009-01-01 00:00:00
abstract::The effect of urocortin (Uro), a recently discovered neuropeptide with selectivity towards corticotropin-releasing hormone type 2 receptor, was tested on whole cell currents expressed by guinea-pig gastric antrum smooth muscle cells. Uro (1 pmol/l-1 nmol/l) caused a concentration-dependent increase of Ca2+-sensitive K...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005653218639
更新日期:2000-01-01 00:00:00
abstract::Skeletal muscle tissue is mechanosensitive, as it is able to sense mechanical impacts and to translate these into biochemical signals making the tissue adapt. Among its mechanosensitive nature, skeletal muscle tissue is the largest metabolic organ of the human body. Disturbances in skeletal muscle mechanosensing and m...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-017-9489-1
更新日期:2017-08-01 00:00:00
abstract::This paper studies human gallbladder (GB) smooth muscle contractions. A two-state cross-bridge model was used to estimate the apparent attachment and detachment rate constants, as well as increased Ca2+ concentration from the peak active stress during the isometric contraction. The active stress was computed from a me...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-011-9260-y
更新日期:2011-11-01 00:00:00
abstract::Under muscle disuse conditions decrease of expression of MyHC of slow type, and sometimes of type IIa, as well as upregulation of expression of IIb and IId/x isoforms were observed. Through dephosphorylation and entry of NFAT molecules to the nucleus calcineurin/NFATc1 signaling pathway promotes upregulation of the sl...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-015-9428-y
更新日期:2016-04-01 00:00:00
abstract::Hodges and his colleagues identified a 12 amino acid fragment of troponin I (TnI-ip) that inhibits Ca(2+)-activated force and reduces the effectiveness Ca(2+) as an activator. To understand the role of troponin C (TnC) in the extended cooperative interactions of thin filament activation, we compared the effect of TnI-...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-013-9336-y
更新日期:2013-05-01 00:00:00
abstract::The hypothesis that calmodulin (CaM) may act as a positive modulator of junctional SR Ca2+-release channel/ ryanodine receptor (RyRl) rests largerly on the demonstrated capacity of CaM to interact structurally and functionally with RyRl at pCa > 8 (Tripathy et al., 1995). The goal of the present [3H]-ryanodine binding...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005635330773
更新日期:2000-01-01 00:00:00
abstract::In this study alterations are characterized which occur, in myocardial force development morphological appearance and protein composition, during the development of cardiac hypertrophy and heart failure in monocrotaline (MCT) treated rats. The transition from cardiac hypertrophy to heart failure was studied by compari...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1019988815436
更新日期:2002-01-01 00:00:00
abstract::Melatonin (N-acetyl-5-methoxy-tryptamine) is an effective antioxidant and free radical scavenger, that has important biological effects in multiple cell types and species. Melatonin research in muscle has recently gained attention, mainly focused on its role in cells or tissue repair and regeneration after injury, due...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-020-09578-3
更新日期:2020-09-01 00:00:00
abstract::Long-(SL > 6 microns) and short-sarcomere (SL < 4 microns) fibres were isolated from the claw muscle of the yabby (Cherax destructor) during limb regeneration and at different stages of the moult cycle. Long-sarcomere fibres were more susceptible to the changes resulting from the moult-induced atrophy compared with th...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00121136
更新日期:1995-06-01 00:00:00
abstract::We investigated the expression of neuronal nitric oxide synthase (nNOS) and dystrophin in the regenerating skeletal muscles of rats after cardiotoxin-induced myonecrosis by immunohistochemical studies and western blot analysis. In normal muscles, nNOS was moderately immunostained on type 2B fibers, but was faintly imm...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1020268700730
更新日期:2002-01-01 00:00:00
abstract::The term 'microtubule dynamics' is often used to describe assembly/disassembly characteristics of this important cytoskeletal polymer. The ability to image microtubules in live Dictyostelium cells has revealed additional dynamic components, acting on the individual assembled tubules. At least two separate forces are i...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1023/a:1024446821701
更新日期:2002-01-01 00:00:00
abstract::To study the development of muscle-specific features during myogenesis, we analysed the ultrastructure and voltage-dependent currents of frog embryonic skeletal myocytes maintained in culture for 10 days. The cells were maintained under culture conditions that prevented cell division, fusion and cell contacts with neu...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005506216009
更新日期:1999-08-01 00:00:00
abstract::The remodelling of connective tissue components is a fundamental requirement for a number of pivotal processes in cell biology. These may include myoblast migration and fusion during development and regeneration. In other systems, similar biological processes are facilitated by secretion of the matrix metalloproteinas...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005670507906
更新日期:2000-04-01 00:00:00