Abstract:
:Shiga toxin-producing Escherichia coli (STEC) strains are responsible for causing hemolytic-uremic syndrome (HUS), and systemic administration of Shiga toxin (Stx)-specific human monoclonal antibodies (HuMAbs) is considered a promising approach for prevention or treatment of the disease in children. The goal of the present study was to investigate the ability of Stx2-specific HuMAbs to protect against infections with STEC strains that produce Stx2 variants. Dose-response studies on five HuMAbs, using the mouse toxicity model, revealed that only the three directed against the A subunit were protective against Stx2 variants, and 5C12 was the most effective among the three tested. Two HuMAbs directed against the B subunit, while highly effective against Stx2, were ineffective against Stx2 variants. In a streptomycin-treated mouse model, parenteral administration of 5C12 significantly protected mice up to 48 h after oral bacterial challenge. We conclude that 5C12, reactive against the Stx2 A subunit, is an excellent candidate for immunotherapy against HUS and that antibodies directed against the A subunit of Stx2 have broad-spectrum activity that includes Stx2 variants, compared with those directed against the B subunit.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Sheoran AS,Chapman S,Singh P,Donohue-Rolfe A,Tzipori Sdoi
10.1128/iai.71.6.3125-3130.2003keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
3125-30issue
6eissn
0019-9567issn
1098-5522journal_volume
71pub_type
杂志文章abstract::We are currently studying the soluble egg antigens of Schistosoma japonicum in an attempt to determine which antigens are potent immunogens. Previously, we demonstrated by Ouchterlony immunodiffusion and inhibition of the circumoval precipitin test that the glycoprotein fraction of soluble egg antigens contains the an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.397-406.1981
更新日期:1981-11-01 00:00:00
abstract::Periodontitis is a bacterium-induced chronic inflammation that destroys tissues that attach teeth to jaw bone. Pathologically excessive matrix metalloproteinase 8 (MMP-8) is among the key players in periodontal destruction by initiating type I collagen degradation. We studied MMP-8 in Porphyromonas gingivalis-induced ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00873-08
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01389-15
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3755-3761.2001
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4485-4488.1993
更新日期:1993-10-01 00:00:00
abstract::Specific proteolytic destruction of the human chemotaxin, C5a, is a property of group A and B streptococcal pathogens. Here we show that virulent group G streptococci from human sources also express C5a peptidase activity. The enzyme responsible for this activity is approximately the same size as and is antigenically ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2305-2310.1991
更新日期:1991-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5267-5282.1992
更新日期:1992-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.56-61.1984
更新日期:1984-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4358-4360.2000
更新日期:2000-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02537-14
更新日期:2014-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3172-3179.2000
更新日期:2000-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.706-715.1982
更新日期:1982-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01212-10
更新日期:2011-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2353-2355.1996
更新日期:1996-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4540-4542.1995
更新日期:1995-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.612-619.2002
更新日期:2002-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.60-63.1982
更新日期:1982-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01458-06
更新日期:2007-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2552-2561.2006
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.949-957.2004
更新日期:2004-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.2119-2121.1994
更新日期:1994-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.11.6129-6139.2002
更新日期:2002-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.749-752.1987
更新日期:1987-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.704-710.1975
更新日期:1975-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2541-2543.1992
更新日期:1992-06-01 00:00:00
abstract::Despite effective antimicrobial chemotherapy, control of Chlamydia trachomatis urogenital infection will likely require a vaccine. We have assessed the protective effect of an outer membrane protein-based vaccine by using a murine model of chlamydial genital infection. Female mice were first vaccinated with Chlamydia ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00622-10
更新日期:2010-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1648-1652.2002
更新日期:2002-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.6.976-981.1972
更新日期:1972-06-01 00:00:00
abstract::Analysis of the specificity of bactericidal antibodies in normal, convalescent, and postvaccination human sera is important in understanding human immunity to meningococcal infections and can aid in the design of an effective group B vaccine. A collection of human sera, including group C and group B convalescent-phase...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05125-11
更新日期:2011-10-01 00:00:00
abstract::Isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with acrylamide copolymerized with gelatin (substrate-SDS-PAGE) were combined to evaluate the proteinases of both long-term-grown and fresh isolates of Trichomonas vaginalis. This two-dimensional substrate-SDS-PAGE resolved a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.157-162.1990
更新日期:1990-01-01 00:00:00