Abstract:
OBJECTIVE:This study was designed to systematically determine whether and to what extent the frequency of intermittent loading modulates the biosynthesis and release of proteoglycans (PGs), and to assess chondrocyte viability within mature bovine articular cartilage explants exposed to different loading patterns. METHODS:Cultured full-thickness cartilage explants from the weight-bearing area of healthy bovine fetlock joints were exposed to intermittently applied, uniaxial cyclic loads by introducing a sinusoidal waveform of 0.1, 0.5 or 1.0Hz, frequency and a peak stress of 0.5MPa for a period of 6 days. The cyclic loads were applied for 5, 10 or 20s followed by a period of unloading lasting 10, 100 or 1000s. The incorporation of radiolabeled sulfate into glycosaminoglycans (GAGs) during the final 18h, the content of GAGs and DNA, the deformation of loaded explants as well as the viability of chondrocytes within the different zones of explants were determined. RESULTS:PG synthesis and loss of endogenous PGs were non-linearly and independently regulated by the frequency of the chosen intermittent load, whereas the release of newly synthesized PGs remained unaffected. The viability of chondrocytes within the superficial zone decreased drastically under intermittent loading in a manner independent of the frequency applied. CONCLUSIONS:Our results confirm the hypothesis that the frequency of intermittent loading is an important mechanical factor controlling the metabolic activities of chondrocytes. They also implicate that an initially healthy cartilage explant can be mechanically manipulated to generate an in vitro model of degenerative, osteoarthritic-like cartilage.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Sauerland K,Raiss RX,Steinmeyer Jdoi
10.1016/s1063-4584(03)00007-4keywords:
subject
Has Abstractpub_date
2003-05-01 00:00:00pages
343-50issue
5eissn
1063-4584issn
1522-9653pii
S1063458403000074journal_volume
11pub_type
杂志文章abstract:INTRODUCTION:Matrix metalloproteinases (MMPs) and 'aggrecanase' a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTSs) are well established to play key roles in osteoarthritis (OA) through degradation of extracellular matrix (ECM) type II collagen and aggrecan, and are thus potential targets for devel...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2017.02.791
更新日期:2017-07-01 00:00:00
abstract::Recent analyses of Col2a1-Cre; ROSA26R reporter mice showed that synovial fibroblasts in 7-day mice were LacZ positive, due to a history of Col2a1-Cre expression conferred by their origin in the interzone of the developing joint. We have examined LacZ staining in adult Col2a1-Cre(+/0); ROSA26R(LacZ) mice, with and wit...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2012.11.013
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:To assess reliability and discriminative validity of cartilage compositional magnetic resonance imaging (MRI) in knee osteoarthritis (OA). DESIGN:The study was carried out per PRISMA recommendations. We searched MEDLINE and EMBASE (1974 - present) for eligible studies. We performed qualitative synthesis of r...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,meta分析
doi:10.1016/j.joca.2017.11.018
更新日期:2018-09-01 00:00:00
abstract:PURPOSE:Biomarkers provide useful diagnostic information by detecting cartilage degradation in osteoarthritis (OA), reflecting disease-relevant biological activity and predicting the course of disease progression. They also serve as surrogate endpoints in the drug discovery process. The aim of this narrative review was...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2012.07.009
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:Increased pro-inflammatory cytokines and reactive oxygen and nitrogen species (RONS) occur in osteoarthritis (OA). Oxygen tension can alter the levels of RONS induced by interleukin-1 (IL-1). RONS such as nitric oxide (NO) can alter energy metabolism. The aim of this study was to determine if oxygen tension a...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2010.06.004
更新日期:2010-09-01 00:00:00
abstract:OBJECTIVE:To employ a magnetic resonance (MR) imaging technique for quantitative assessment of thin cartilage layers, and to validate the cartilage volume and thickness measurements. METHODS:We investigated 10 normal elbow joints (age 20 to 69 years) with a 3D gradient echo sequence with selective water excitation (TR...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1053/joca.1999.0278
更新日期:2000-03-01 00:00:00
abstract:INTRODUCTION:Osteoarthritis (OA) is a common disease worldwide leading to significant morbidity. The underlying disease process is multifactorial however there is increasing focus on molecular mechanisms. MicroRNAs are small non-coding segments of RNA that have important regulatory functions at a cellular level. These ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2015.10.018
更新日期:2016-04-01 00:00:00
abstract:OBJECTIVE:The relative differences in effectiveness of subchondral stimulation, osteochondral grafts, and autologous chondrocyte implantation (ACI) are still unclear. It is the objective of this study to systematically review the literature on ACI compared to other treatments by clinical outcome and the quality of the ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2010.03.005
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:Reliable evaluation of joint space width and subchondral sclerosis of osteoarthritic joints is difficult. The present study describes a new digital method to analyse standard radiographs of the ankle. DESIGN:Standardized radiographs were taken of the ankle of 12 patients with severe osteoarthritis (OA) under...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1053/joca.2000.0384
更新日期:2001-04-01 00:00:00
abstract:OBJECTIVE:Osteoarthritic cartilage destruction can be regulated by the balance between proteases and anti-proteases. Here, we sought to identify novel cellular protease inhibitors associated with osteoarthritis (OA) pathogenesis. METHODS:Candidate molecules were screened from microarray data of chondrocytes treated wi...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2021.01.003
更新日期:2021-01-21 00:00:00
abstract:OBJECTIVE:To investigate the mechanical properties and morphological characteristics of articular cartilage on the tibial plateau of human knees, including the region covered by the meniscus. DESIGN:Using a 1-mm diameter flat-ended cylindrical probe to apply a constant load (0.6 MPa) at specific sites on the tibial pl...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2006.01.015
更新日期:2006-06-01 00:00:00
abstract:OBJECTIVE:This study used a rat model of post-traumatic knee osteoarthritis (OA) created by anterior cruciate ligament transection with partial medial meniscectomy (ACLT + pMMx). In this model, mild to moderate structural changes that are typical of knee OA have been observed within 2 and 8 weeks post-surgery. We aimed...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.03.001
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVES:Using a mouse surgical model of post-traumatic osteoarthritis (OA), we sought to determine if muscle function is altered following acute joint injury and whether this relates to OA progression. DESIGN:Male C57BL/6 mice underwent surgical transection of the medio-meniscal tibial ligament destabilisation of t...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.12.008
更新日期:2016-06-01 00:00:00
abstract::Variations among joints in the initiation and progression of degeneration may be explained, in part, by metabolic, biochemical and biomechanical differences. Compared to the cartilage in the knee joint, ankle cartilage has a higher content of proteoglycans and water, as well as an increased rate of proteoglycan turnov...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2004.11.006
更新日期:2005-02-01 00:00:00
abstract::Although the multifactorial nature of osteoarthritis (OA) is well recognized, genetic factors have been found to be strong determinants of the disease. Evidence of a genetic influence of OA comes from a number of sources, including epidemiological studies of family history and family clustering, twin studies, and expl...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2003.09.005
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVE:To validate an automatic scheme for the segmentation and quantitative analysis of the medial meniscus (MM) and lateral meniscus (LM) in magnetic resonance (MR) images of the knee. METHOD:We analysed sagittal water-excited double-echo steady-state MR images of the knee from a subset of the Osteoarthritis Init...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2014.06.029
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:Articular cartilage matrix synthesis and degradation are dynamic processes that must be balanced for proper maintenance of the tissue. In osteoarthritis (OA), this balance is skewed toward degradation and ultimate loss of matrix. The transcriptional and/or activity levels of hundreds of genes are dysregula...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2004.05.001
更新日期:2004-08-01 00:00:00
abstract:OBJECTIVE:To explore effects of weight loss and maintenance on serum cartilage biomarkers denaturation neoepitope for Collagen2 (Coll2-1) and Fibulin3 fragment (Fib3-2), as well as correlations between Coll2-1 and Fib3-2 and symptomatic improvement, in a knee osteoarthritis (KOA) population. DESIGN:192 obese KOA patie...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joca.2017.06.009
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to determine the effects of intra-articular injections of high molecular weight (2000 kDa) sodium hyaluronate (HA) on the progression of articular cartilage degeneration in a rabbit partial medial meniscectomy model of osteoarthritis. DESIGN:Six experimental groups included norm...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2003.11.007
更新日期:2004-03-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to assess the reproducibility of meniscus T1rho measurements, and to study T1rho relaxation time in the lateral meniscus (LM) and its relationship with adjacent cartilage T1rho in knees with acute anterior cruciate ligament (ACL) injuries at 3T magnetic resonance imaging. METHOD:Qua...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2008.05.016
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVE:Macromolecules of the articular cartilage extracellular matrix released into synovial fluid, blood, or urine can serve as potentially useful biomarkers of the severity of osteoarthritis (OA). Biomechanical factors play an important role in OA pathogenesis, yet their influence on biomarker production is not we...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2005.07.003
更新日期:2005-12-01 00:00:00
abstract:OBJECTIVE:To evaluate anterior cruciate ligament transection (ACLT) and destabilization of the medial meniscus (DMM) surgical instability models of osteoarthritis (OA) in the 129/SvEv mouse knee joint. DESIGN:Micro-surgical techniques were used to perform ACLT or DMM under direct visualization. Histological scoring wa...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2007.03.006
更新日期:2007-09-01 00:00:00
abstract:OBJECTIVE:To investigate the in vivo role of the IRE1/XBP1 unfolded protein response (UPR) signaling pathway in cartilage. DESIGN:Xbp1(flox/flox).Col2a1-Cre mice (Xbp1(CartΔEx2)), in which XBP1 activity is ablated specifically from cartilage, were analyzed histomorphometrically by Alizarin red/Alcian blue skeletal pre...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.01.001
更新日期:2015-04-01 00:00:00
abstract:OBJECTIVE:Slipped capital femoral epiphysis (SCFE) in adolescence is associated with increased risk of future osteoarthritis (OA). The purpose of this study was to study clinical and radiographic risk factors for early cartilage degeneration after SCFE. DESIGN:22 patients (44 hips) (mean age 24 years, range 18-27) tre...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2018.01.013
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVE:Partial meniscectomy is the golden standard for treating a bucket-handle tear in the meniscus of the knee, but it inevitably leads to articular cartilage degeneration. Surgical creation of an access channel between the lesion and the vascularized synovial lining is intended to induce ingrowth of repair tissue...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1053/joca.2002.0870
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVE:To study the effect of 17β-estradiol (E2) and the superficial zone (SFZ) on cell death and proteoglycan degradation in articular cartilage after a single injurious compression in vitro. METHOD:Cartilage explants from the femoropatellar groove of 2 year old cows with or without the SFZ were cultured serum-fre...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2013.07.007
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVE:To determine the within- and between-patient variability in the concentrations of synovial fluid, serum and urine markers of joint tissue metabolism in a cohort of patients with knee pain and cartilage changes consistent with early-stage knee osteoarthritis. DESIGN:Samples of synovial fluid, serum, and urine...
journal_title:Osteoarthritis and cartilage
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1053/joca.1998.0134
更新日期:1998-09-01 00:00:00
abstract:OBJECTIVE:Abnormal joint instability contributes to cartilage damage and osteophyte formation. We investigated whether controlling joint instability inhibited chronic synovial membrane inflammation and delayed osteophyte formation and examined the role of transforming growth factor-beta (TGF-β) signaling in the associa...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2019.03.008
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVE:Extracellular inorganic pyrophosphate (ePPi) plays a key role in the regulation of normal and pathologic mineralization. The purpose of this work was to evaluate the role of P1 and P2 purine receptors in modulating ePPi production by articular chondrocytes. METHODS:Porcine cartilage explants and chondrocyte ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2010.08.004
更新日期:2010-11-01 00:00:00
abstract::Our objective was to investigate the factors that control chondrocyte division, namely, (1) steric hindrance caused by the surrounding matrix, (2) growth factors and (3) cell shape. Bovine articular cartilage explants and isolated chondrocytes were cultured in medium containing [3H]-TdR to measure DNA synthesis. The e...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/s1063-4584(05)80029-9
更新日期:1993-04-01 00:00:00